TMN1_ARATH
ID TMN1_ARATH Reviewed; 589 AA.
AC Q940G0; O04091; Q0WMB1;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Transmembrane 9 superfamily member 1 {ECO:0000305};
DE AltName: Full=Endomembrane protein 12 {ECO:0000303|PubMed:22570441};
DE AltName: Full=Transmembrane nine protein 1 {ECO:0000303|PubMed:20681974};
DE Short=AtTMN1 {ECO:0000303|PubMed:20681974};
DE Flags: Precursor;
GN Name=TMN1 {ECO:0000303|PubMed:20681974};
GN Synonyms=EMP12 {ECO:0000303|PubMed:22570441};
GN OrderedLocusNames=At1g10950 {ECO:0000312|Araport:AT1G10950};
GN ORFNames=T19D16.13 {ECO:0000312|EMBL:AAB65482.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAK96857.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 454-589.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=20681974; DOI=10.1111/j.1399-3054.2010.01404.x;
RA Hegelund J.N., Jahn T.P., Baekgaard L., Palmgren M.G., Schjoerring J.K.;
RT "Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal
RT contents without changing vacuolar morphology.";
RL Physiol. Plantarum 140:355-367(2010).
RN [6]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, MOTIF, AND MUTAGENESIS OF
RP 578-PHE-VAL-579; TYR-583; LYS-587 AND ASP-589.
RX PubMed=22570441; DOI=10.1105/tpc.112.096057;
RA Gao C., Yu C.K., Qu S., San M.W., Li K.Y., Lo S.W., Jiang L.;
RT "The Golgi-localized Arabidopsis endomembrane protein12 contains both
RT endoplasmic reticulum export and Golgi retention signals at its C
RT terminus.";
RL Plant Cell 24:2086-2104(2012).
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P32802};
CC Multi-pass membrane protein {ECO:0000255}. Golgi apparatus membrane
CC {ECO:0000269|PubMed:22570441}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:20681974}.
CC -!- DOMAIN: The C-terminal KXD/E motif functions as a Golgi retention
CC signal, certainly through the binding to the COP1 coatomer.
CC {ECO:0000269|PubMed:22570441}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB65482.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U95973; AAB65482.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28668.1; -; Genomic_DNA.
DR EMBL; AF446365; AAL48237.1; -; mRNA.
DR EMBL; AY054666; AAK96857.1; -; mRNA.
DR EMBL; AY063907; AAL36263.1; -; mRNA.
DR EMBL; AY081536; AAM10098.1; -; mRNA.
DR EMBL; BT008600; AAP40425.1; -; mRNA.
DR EMBL; AK229917; BAF01745.1; -; mRNA.
DR PIR; D86243; D86243.
DR RefSeq; NP_563881.1; NM_100970.4.
DR AlphaFoldDB; Q940G0; -.
DR BioGRID; 22878; 1.
DR STRING; 3702.AT1G10950.1; -.
DR TCDB; 8.A.68.1.7; the endomembrane protein-70 (emp70) family.
DR iPTMnet; Q940G0; -.
DR PaxDb; Q940G0; -.
DR PRIDE; Q940G0; -.
DR ProteomicsDB; 234440; -.
DR EnsemblPlants; AT1G10950.1; AT1G10950.1; AT1G10950.
DR GeneID; 837638; -.
DR Gramene; AT1G10950.1; AT1G10950.1; AT1G10950.
DR KEGG; ath:AT1G10950; -.
DR Araport; AT1G10950; -.
DR TAIR; locus:2197364; AT1G10950.
DR eggNOG; KOG1277; Eukaryota.
DR HOGENOM; CLU_010714_0_2_1; -.
DR InParanoid; Q940G0; -.
DR OMA; APCRVNA; -.
DR OrthoDB; 641127at2759; -.
DR PhylomeDB; Q940G0; -.
DR PRO; PR:Q940G0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q940G0; baseline and differential.
DR Genevisible; Q940G0; AT.
DR GO; GO:0005801; C:cis-Golgi network; IDA:TAIR.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000137; C:Golgi cis cisterna; HDA:TAIR.
DR GO; GO:0005797; C:Golgi medial cisterna; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR InterPro; IPR004240; EMP70.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
PE 1: Evidence at protein level;
KW Endosome; Golgi apparatus; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..589
FT /note="Transmembrane 9 superfamily member 1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431258"
FT TOPO_DOM 25..222
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 223..243
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 294..314
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 315..321
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 322..342
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..364
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 365..385
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 386..395
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 396..416
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 449..469
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 470..481
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 482..502
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 503..518
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 519..539
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 540..550
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 551..571
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 572..589
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOTIF 578..583
FT /note="Endoplasmic reticulum export signal"
FT /evidence="ECO:0000269|PubMed:22570441"
FT MOTIF 587..589
FT /note="Golgi retention signal"
FT /evidence="ECO:0000269|PubMed:22570441"
FT MUTAGEN 578..579
FT /note="FV->AA: Abolishes export from endoplasmic reticulum;
FT when associated with Ala-583."
FT /evidence="ECO:0000269|PubMed:22570441"
FT MUTAGEN 583
FT /note="Y->A: Abolishes export from endoplasmic reticulum;
FT when associated with Ala-578-579-Ala."
FT /evidence="ECO:0000269|PubMed:22570441"
FT MUTAGEN 587..589
FT /note="KCD->ACA: Abolishes Golgi retention."
FT /evidence="ECO:0000269|PubMed:22570441"
FT MUTAGEN 587
FT /note="K->A: Abolishes Golgi retention."
FT /evidence="ECO:0000269|PubMed:22570441"
FT MUTAGEN 589
FT /note="D->A: Abolishes Golgi retention."
FT /evidence="ECO:0000269|PubMed:22570441"
SQ SEQUENCE 589 AA; 66893 MW; 21E915622423BECD CRC64;
MPSSSSAAVL VFLLLVSLLT PTFASDSDHK YQAEEQVTLW VNKVGPYNNP QETYNYYSLP
FCRPSGNNVH KWGGLGEVLG GNELIDSEIA IKFMKNVERS VICPLELDEA KVKHFKDAIE
SSYWFEFFMD DLPLWGFVGE LHPDKNSENG KHVLYTHKNI VVKYNKDQII HVNLTQDNPR
PLEAGKKMDL TYSVQWIPTN VTFARRFDVY LDYPFFEHQI HWFSIFNSFM MVIFLTGLVS
MILMRTLRND YAKYAREDDD LESLERDVSE ESGWKLVHGD VFRPASSLVL LSAVVGTGAQ
LALLVLLVIL MAIVGTLYVG RGAIVTTFIV CYALTSFVSG YVSGGMYSRS GGKHWIKCMV
LTASLFPFLC FGIGFLLNTI AIFYGSLAAI PFGTMVVVFV IWGFISFPLA LLGTVVGRNW
SGAPNNPCRV KTIPRPIPEK KWYLTPSVVS LMGGLLPFGS IFIEMYFVFT SFWNYKVYYV
YGFMLLVFVI LVIVTVCVTI VGTYFLLNAE NYHWQWTSFF SAASTAVYVY LYSIYYYYVK
TKMSGFFQTS FYFGYTMMFC LGLGILCGAV GYLGSNLFVR RIYRNIKCD
