TMN2_YEAST
ID TMN2_YEAST Reviewed; 672 AA.
AC Q04562; D6VS93;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Transmembrane 9 superfamily member 2;
DE Flags: Precursor;
GN Name=TMN2; OrderedLocusNames=YDR107C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [4]
RP FUNCTION.
RX PubMed=18178563; DOI=10.1074/jbc.m704484200;
RA Froquet R., Cherix N., Birke R., Benghezal M., Cameroni E., Letourneur F.,
RA Moesch H.-U., De Virgilio C., Cosson P.;
RT "Control of cellular physiology by TM9 proteins in yeast and
RT Dictyostelium.";
RL J. Biol. Chem. 283:6764-6772(2008).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=20681974; DOI=10.1111/j.1399-3054.2010.01404.x;
RA Hegelund J.N., Jahn T.P., Baekgaard L., Palmgren M.G., Schjoerring J.K.;
RT "Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal
RT contents without changing vacuolar morphology.";
RL Physiol. Plantarum 140:355-367(2010).
CC -!- FUNCTION: With EMP70 and TMN3, plays a critical role in the late stages
CC of a nutrient-controlled pathway notably regulating FLO11 gene
CC expression. Acts downstream of RAS2 and TOR. Essential for cell
CC adhesion and filamentous growth. May play a role as effector of
CC cellular copper homeostasis. {ECO:0000269|PubMed:18178563,
CC ECO:0000269|PubMed:20681974}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z48758; CAA88661.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11953.1; -; Genomic_DNA.
DR PIR; S52673; S52673.
DR RefSeq; NP_010392.1; NM_001180415.1.
DR AlphaFoldDB; Q04562; -.
DR BioGRID; 32165; 55.
DR DIP; DIP-4247N; -.
DR IntAct; Q04562; 3.
DR MINT; Q04562; -.
DR STRING; 4932.YDR107C; -.
DR iPTMnet; Q04562; -.
DR MaxQB; Q04562; -.
DR PaxDb; Q04562; -.
DR PRIDE; Q04562; -.
DR EnsemblFungi; YDR107C_mRNA; YDR107C; YDR107C.
DR GeneID; 851685; -.
DR KEGG; sce:YDR107C; -.
DR SGD; S000002514; TMN2.
DR VEuPathDB; FungiDB:YDR107C; -.
DR eggNOG; KOG1278; Eukaryota.
DR GeneTree; ENSGT00940000172441; -.
DR HOGENOM; CLU_010714_4_1_1; -.
DR InParanoid; Q04562; -.
DR OMA; CYPKNGT; -.
DR BioCyc; YEAST:G3O-29709-MON; -.
DR PRO; PR:Q04562; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04562; protein.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0006878; P:cellular copper ion homeostasis; IDA:UniProtKB.
DR GO; GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR GO; GO:0007124; P:pseudohyphal growth; IGI:SGD.
DR GO; GO:0007034; P:vacuolar transport; IGI:SGD.
DR InterPro; IPR004240; EMP70.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
PE 1: Evidence at protein level;
KW Ion transport; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..672
FT /note="Transmembrane 9 superfamily member 2"
FT /id="PRO_0000244444"
FT TOPO_DOM 19..307
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..383
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 405..410
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 432..447
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..479
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 501..532
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 554..565
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..601
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 623..628
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 629..649
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 650..672
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 672 AA; 76347 MW; 172855FA4B29BA13 CRC64;
MKRGVWLLIY CYATLTKGFS LPGLSPTTYH SGDEIPLLVN RLTPSIYFQH QDEEGNDVSG
DKEHFLYSYD YYNKRFHFCR PEHVEKQPES LGSVIFGDRI YNSPFQLNML EEKECVALCK
STIPGKDAKF INTLIKSGFF QNWLVDGLPA ARKAYDSRTK TNYYGTGFEL GFTDVKQTVD
GKAVPSTMEE LTSEASNEDV ILDARLPKNV KPNLVKTVEL PYFVNHFDIE VEFHDRGNDN
YRVVGVIVNP VSIERSSPGA CSTTGKPLIL DEDKDNEVYF TYSVKFVASD TVWATRWDKY
LHIYDPQIQW FSLINFSVIV ILLSSVVMHS LLRALKSDLA RYNELNLDNE FHEDSGWKLG
HGDVFRTPSK SMLLSILVGS GMQLFLMVMC SIFFAAVGLV SPVSRGSLPT VMFVLYALFG
FVGSYASMGV YKFFRGPYWK ANMILTPILL PGAIFLLIVI MNFFLLFAHS SGVIPARSLF
FIILLWFLVS VPLSFAGSIV AHKQCNWDEH PTKTNQIARQ IPYQPWYLRT AQATLIAGIF
SFGSIAVELY FIYSSLWFNK IFYMFGFLLF SFLLLTLTTS LVTILITYYS LCLENWLWQW
RSFIIGGLGC SIYTFIHSIL FTKFKLGGVI TVVLYLGYSL IISALCCVVT GAIGFFSSMF
FIRKIYSAIK VE
