TMN3_ARATH
ID TMN3_ARATH Reviewed; 592 AA.
AC Q9ZPS7; B9DHM0; Q56X26;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Transmembrane 9 superfamily member 3 {ECO:0000305};
DE AltName: Full=Endomembrane protein 9 {ECO:0000303|PubMed:22570441};
DE AltName: Full=Transmembrane nine protein 3 {ECO:0000303|PubMed:20681974};
DE Short=AtTMN3 {ECO:0000303|PubMed:20681974};
DE Flags: Precursor;
GN Name=TMN3 {ECO:0000303|PubMed:20681974};
GN Synonyms=EMP9 {ECO:0000303|PubMed:22570441};
GN OrderedLocusNames=At2g01970 {ECO:0000312|Araport:AT2G01970};
GN ORFNames=F14H20.4 {ECO:0000312|EMBL:AAD20090.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 282-592.
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20681974; DOI=10.1111/j.1399-3054.2010.01404.x;
RA Hegelund J.N., Jahn T.P., Baekgaard L., Palmgren M.G., Schjoerring J.K.;
RT "Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal
RT contents without changing vacuolar morphology.";
RL Physiol. Plantarum 140:355-367(2010).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22570441; DOI=10.1105/tpc.112.096057;
RA Gao C., Yu C.K., Qu S., San M.W., Li K.Y., Lo S.W., Jiang L.;
RT "The Golgi-localized Arabidopsis endomembrane protein12 contains both
RT endoplasmic reticulum export and Golgi retention signals at its C
RT terminus.";
RL Plant Cell 24:2086-2104(2012).
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P32802};
CC Multi-pass membrane protein {ECO:0000255}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q940G0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: The C-terminal KXD/E motif functions as a Golgi retention
CC signal, certainly through the binding to the COP1 coatomer.
CC {ECO:0000250|UniProtKB:Q940G0}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
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DR EMBL; AC006532; AAD20090.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05528.1; -; Genomic_DNA.
DR EMBL; AY058869; AAL24256.1; -; mRNA.
DR EMBL; AK221852; BAD94118.1; -; mRNA.
DR EMBL; AK226487; BAE98629.1; -; mRNA.
DR EMBL; AK317573; BAH20237.1; -; mRNA.
DR PIR; D84431; D84431.
DR RefSeq; NP_178306.1; NM_126258.3.
DR AlphaFoldDB; Q9ZPS7; -.
DR BioGRID; 131; 54.
DR IntAct; Q9ZPS7; 54.
DR STRING; 3702.AT2G01970.1; -.
DR PaxDb; Q9ZPS7; -.
DR PRIDE; Q9ZPS7; -.
DR ProteomicsDB; 234451; -.
DR EnsemblPlants; AT2G01970.1; AT2G01970.1; AT2G01970.
DR GeneID; 814728; -.
DR Gramene; AT2G01970.1; AT2G01970.1; AT2G01970.
DR KEGG; ath:AT2G01970; -.
DR Araport; AT2G01970; -.
DR TAIR; locus:2041160; AT2G01970.
DR eggNOG; KOG1277; Eukaryota.
DR HOGENOM; CLU_010714_0_2_1; -.
DR InParanoid; Q9ZPS7; -.
DR OMA; AKWKETD; -.
DR OrthoDB; 641127at2759; -.
DR PhylomeDB; Q9ZPS7; -.
DR PRO; PR:Q9ZPS7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZPS7; baseline and differential.
DR Genevisible; Q9ZPS7; AT.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR InterPro; IPR004240; EMP70.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Endosome; Golgi apparatus; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..592
FT /note="Transmembrane 9 superfamily member 3"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431260"
FT TOPO_DOM 20..229
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 230..250
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 303..323
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..325
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 326..346
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..365
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 366..386
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 387..397
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 398..418
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..452
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 453..473
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 474..485
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 486..506
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..521
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 522..542
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 543..553
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 554..574
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 575..592
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOTIF 581..586
FT /note="Endoplasmic reticulum export signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
FT MOTIF 590..592
FT /note="Golgi retention signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
SQ SEQUENCE 592 AA; 68049 MW; 710C78B82A7A067C CRC64;
MRLPTTLLLF IGALIFSGAG TVRSDASDHR YKDGDSVPLY ANKVGPFHNP SETYRYFDLP
FCIPEGVKDK KEALGEVLNG DRLVSAPYKL NFRDEKDSEV YCKKKLSREE VEHFRRAVEK
DYYFQMYYDD LPIWGFIGKV DKESKSDPSE FKYFLYKHIQ FEILYNKDRV IEINARMDPH
SLVDLTEDKE VDAEFMYTVK WKETETSFEK RMDKYAMSSS LPHHLEIHWF SIINSCVTVL
LLTGFLATIL MRVLKNDFMK YAQDEEAADD QEETGWKYIH GDVFRFPKNK SLFAASLGSG
TQLFTLTIFI FMLSLVGVFY PYNRGALFTA LVVIYALTSG IAGYTASSFY CQLEGKNWVR
NLLLTGGLFC GPLFLTFCFL NTVAIAYSAT AALPFGTIIV IVLIWTLVTS PLLVLGGIAG
KNSKAEFQAP VRTTKYPREI PPLPWYRSAV PQMAMAGFLP FSAIYIELYY IFASVWGHRI
YTIYSILFIV FIILLIVTAF ITVALTYFQL AAEDHEWWWR SFLCGGSTGL FIYAYCLYYY
YARSDMSGFM QTSFFFGYMA CICYGFFLML GTVGFRAALL FVRHIYRSIK CE
