TMN5_ARATH
ID TMN5_ARATH Reviewed; 589 AA.
AC F4HW17; F4HW18; Q94AV9; Q9SCX1; Q9SJF5;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Transmembrane 9 superfamily member 5 {ECO:0000305};
DE AltName: Full=Endomembrane protein 11 {ECO:0000303|PubMed:22570441};
DE AltName: Full=Transmembrane nine protein 5 {ECO:0000303|PubMed:20681974};
DE Short=AtTMN5 {ECO:0000303|PubMed:20681974};
DE Flags: Precursor;
GN Name=TMN5 {ECO:0000303|PubMed:20681974};
GN Synonyms=DIDI 8C-5D {ECO:0000312|EMBL:CAB62549.1},
GN EMP11 {ECO:0000303|PubMed:22570441};
GN OrderedLocusNames=At1g08350 {ECO:0000312|Araport:AT1G08350};
GN ORFNames=T27G7.5 {ECO:0000312|EMBL:AAF22904.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 524-589 (ISOFORM 1/2), TISSUE SPECIFICITY,
RP AND INDUCTION.
RC TISSUE=Root;
RX PubMed=11277426; DOI=10.1094/mpmi.2001.14.3.288;
RA Vercauteren I., van der Schueren E., Van Montagu M., Gheysen G.;
RT "Arabidopsis thaliana genes expressed in the early compatible interaction
RT with root-knot nematodes.";
RL Mol. Plant Microbe Interact. 14:288-299(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20681974; DOI=10.1111/j.1399-3054.2010.01404.x;
RA Hegelund J.N., Jahn T.P., Baekgaard L., Palmgren M.G., Schjoerring J.K.;
RT "Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal
RT contents without changing vacuolar morphology.";
RL Physiol. Plantarum 140:355-367(2010).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22570441; DOI=10.1105/tpc.112.096057;
RA Gao C., Yu C.K., Qu S., San M.W., Li K.Y., Lo S.W., Jiang L.;
RT "The Golgi-localized Arabidopsis endomembrane protein12 contains both
RT endoplasmic reticulum export and Golgi retention signals at its C
RT terminus.";
RL Plant Cell 24:2086-2104(2012).
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P32802};
CC Multi-pass membrane protein {ECO:0000255}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q940G0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4HW17-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HW17-2; Sequence=VSP_057142;
CC -!- TISSUE SPECIFICITY: Expressed in the root cap and in giant cells.
CC {ECO:0000269|PubMed:11277426}.
CC -!- INDUCTION: Up-regulated upon nematode infection.
CC {ECO:0000269|PubMed:11277426}.
CC -!- DOMAIN: The C-terminal KXD/E motif functions as a Golgi retention
CC signal, certainly through the binding to the COP1 coatomer.
CC {ECO:0000250|UniProtKB:Q940G0}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF22904.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006932; AAF22904.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28279.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28280.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60891.1; -; Genomic_DNA.
DR EMBL; AY045680; AAK74038.1; -; mRNA.
DR EMBL; BT001044; AAN46798.1; -; mRNA.
DR EMBL; AJ249959; CAB62549.1; -; mRNA.
DR RefSeq; NP_001077489.1; NM_001084020.1. [F4HW17-1]
DR RefSeq; NP_001323140.1; NM_001331772.1.
DR RefSeq; NP_001323141.1; NM_001331771.1. [F4HW17-1]
DR RefSeq; NP_563812.1; NM_100708.2. [F4HW17-2]
DR AlphaFoldDB; F4HW17; -.
DR STRING; 3702.AT1G08350.2; -.
DR PaxDb; F4HW17; -.
DR PRIDE; F4HW17; -.
DR EnsemblPlants; AT1G08350.1; AT1G08350.1; AT1G08350. [F4HW17-2]
DR EnsemblPlants; AT1G08350.2; AT1G08350.2; AT1G08350. [F4HW17-1]
DR EnsemblPlants; AT1G08350.3; AT1G08350.3; AT1G08350. [F4HW17-1]
DR GeneID; 837355; -.
DR Gramene; AT1G08350.1; AT1G08350.1; AT1G08350. [F4HW17-2]
DR Gramene; AT1G08350.2; AT1G08350.2; AT1G08350. [F4HW17-1]
DR Gramene; AT1G08350.3; AT1G08350.3; AT1G08350. [F4HW17-1]
DR KEGG; ath:AT1G08350; -.
DR Araport; AT1G08350; -.
DR TAIR; locus:2201806; AT1G08350.
DR eggNOG; KOG1277; Eukaryota.
DR HOGENOM; CLU_010714_0_2_1; -.
DR InParanoid; F4HW17; -.
DR OMA; LYELKFR; -.
DR PRO; PR:F4HW17; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HW17; baseline and differential.
DR Genevisible; F4HW17; AT.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR GO; GO:0009624; P:response to nematode; IEP:UniProtKB.
DR InterPro; IPR004240; EMP70.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endosome; Golgi apparatus; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..589
FT /note="Transmembrane 9 superfamily member 5"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431262"
FT TOPO_DOM 25..227
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 228..248
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 292..312
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..321
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 322..342
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..361
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 362..382
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..394
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 395..415
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..450
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 451..471
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..482
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 483..503
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 504..518
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 519..539
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 540..550
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 551..571
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 572..589
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOTIF 578..583
FT /note="Endoplasmic reticulum export signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
FT MOTIF 587..589
FT /note="Golgi retention signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
FT VAR_SEQ 1..81
FT /note="Missing (in isoform 2)"
FT /id="VSP_057142"
FT CONFLICT 582
FT /note="I -> V (in Ref. 3; AAK74038/AAN46798)"
SQ SEQUENCE 589 AA; 67678 MW; 253DEEACFEDD7A0D CRC64;
MAQFLLTVLQ VLLALTFWIG IGSGSSNHYN AGDHVPLFVN KVGPLHNPSE TYQYYDLPFC
RRGPVIEKQE TLGEVLNGDR LMSSLYKLKF REDKTHFVLC RKRLTSSDIA RFRDIIAQDY
YFQMYYDDLP LWGFVGKVEG DYFGQGEKHT KYYIFSHLKF NVLYNADKVI EINSFSDPSY
MVDISENTEI DVQFTYSVSW NLTSERSETR MNKYSRASFH PISQKIHFFS FLNSITVVVL
LIGLISFLFM RHLKNELRSY SIGDEEERKE AGWKLVHSDV FRCPRNISWL CAILGTGTQL
LILIIALFAL AFTGFLYPYN RGMLLTSLVI MYTLTSIVAG YTSTSFHSQF EGNKQKRSVR
LAGILYPVPF FIILSVLNTV AITYGATAAL PFGTIVIIIL IFTLLNIPFL MLGGVLGNRF
GLLEFQPPSA VKRNPREIPP QNWYRRKLYQ VFLGGFVPFS AVVLEWHQLY ASLWGFKIYT
SPGIMLFTFI VLIFLSSSVG IILTYIQLSG EDHEWWWRSI LCGGFTAVFM YGYGVLFYLR
SDMTGFLQLS FYLGYTALLC YALFLVLGTI SFLASLMFIR HIYRSVKLE