TMN6_ARATH
ID TMN6_ARATH Reviewed; 637 AA.
AC Q9C720; Q84WT7;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Transmembrane 9 superfamily member 6 {ECO:0000305};
DE AltName: Full=Endomembrane protein 4 {ECO:0000303|PubMed:22570441};
DE AltName: Full=Transmembrane nine protein 6 {ECO:0000303|PubMed:20681974};
DE Short=AtTMN6 {ECO:0000303|PubMed:20681974};
DE Flags: Precursor;
GN Name=TMN6 {ECO:0000303|PubMed:20681974};
GN Synonyms=EMP4 {ECO:0000303|PubMed:22570441};
GN OrderedLocusNames=At1g55130 {ECO:0000312|Araport:AT1G55130};
GN ORFNames=T7N22.7 {ECO:0000312|EMBL:AAG50838.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 313-637.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=20681974; DOI=10.1111/j.1399-3054.2010.01404.x;
RA Hegelund J.N., Jahn T.P., Baekgaard L., Palmgren M.G., Schjoerring J.K.;
RT "Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal
RT contents without changing vacuolar morphology.";
RL Physiol. Plantarum 140:355-367(2010).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22570441; DOI=10.1105/tpc.112.096057;
RA Gao C., Yu C.K., Qu S., San M.W., Li K.Y., Lo S.W., Jiang L.;
RT "The Golgi-localized Arabidopsis endomembrane protein12 contains both
RT endoplasmic reticulum export and Golgi retention signals at its C
RT terminus.";
RL Plant Cell 24:2086-2104(2012).
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:P32802};
CC Multi-pass membrane protein {ECO:0000255}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q940G0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous but weakly expressed in flowers and
CC stems. {ECO:0000269|PubMed:20681974}.
CC -!- DOMAIN: The C-terminal KXD/E motif functions as a Golgi retention
CC signal, certainly through the binding to the COP1 coatomer.
CC {ECO:0000250|UniProtKB:Q940G0}.
CC -!- SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.
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DR EMBL; AC073944; AAG50838.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33189.1; -; Genomic_DNA.
DR EMBL; BT002777; AAO22605.1; -; mRNA.
DR PIR; H96592; H96592.
DR RefSeq; NP_175909.1; NM_104386.3.
DR AlphaFoldDB; Q9C720; -.
DR BioGRID; 27181; 2.
DR STRING; 3702.AT1G55130.1; -.
DR PaxDb; Q9C720; -.
DR PRIDE; Q9C720; -.
DR ProteomicsDB; 234433; -.
DR EnsemblPlants; AT1G55130.1; AT1G55130.1; AT1G55130.
DR GeneID; 841956; -.
DR Gramene; AT1G55130.1; AT1G55130.1; AT1G55130.
DR KEGG; ath:AT1G55130; -.
DR Araport; AT1G55130; -.
DR TAIR; locus:2205682; AT1G55130.
DR eggNOG; KOG1278; Eukaryota.
DR HOGENOM; CLU_010714_4_1_1; -.
DR InParanoid; Q9C720; -.
DR OMA; LEDQPCR; -.
DR OrthoDB; 641127at2759; -.
DR PhylomeDB; Q9C720; -.
DR PRO; PR:Q9C720; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C720; baseline and differential.
DR Genevisible; Q9C720; AT.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0072657; P:protein localization to membrane; IBA:GO_Central.
DR InterPro; IPR004240; EMP70.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10766; PTHR10766; 1.
DR Pfam; PF02990; EMP70; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Endosome; Golgi apparatus; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..637
FT /note="Transmembrane 9 superfamily member 6"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431263"
FT TOPO_DOM 24..274
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 275..295
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..344
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 345..365
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 366..370
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 371..391
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..411
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 412..432
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..444
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 445..465
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 466..495
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 496..516
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 517..527
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 528..548
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 549..565
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 566..586
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..593
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 594..614
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 615..637
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOTIF 626..631
FT /note="Endoplasmic reticulum export signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
FT MOTIF 635..637
FT /note="Golgi retention signal"
FT /evidence="ECO:0000250|UniProtKB:Q940G0"
SQ SEQUENCE 637 AA; 73623 MW; AFCA06D2295CAD04 CRC64;
MAIRIRISGT LLLSFLFFST LHAFYLPGVA PRDFQKGDPL YVKVNKLSST KTQLPYDFYY
LNYCKPPKIL NTGENLGEVL RGDRIENSVY TFEMLEDQPC RVGCRVRVDA ESAKNFREKI
DYEYRANMIL DNLPVAVLRQ RKDGIQSTTY EHGYRVGFKG SYEGSKEKKY FIHNHLSFRV
MYHRDQESES SRIVGFEVTP NSVLHEYKEW DENNPQLTTC NKDTKNLIQS NTVPQEVEEG
KEIVFTYDVA FKESVIKWAS RWDTYLLMND DQIHWFSIIN SLMIVLFLSG MVAMIMMRTL
YKDISNYNQL ETQDEAQEET GWKLVHGDVF RTPMNSGLLC VYVGTGVQIF GMTLVTMIFA
LLGFLSPSNR GGLTTAMVLL WVFMGIFAGY SSSRLHKMFK GNEWKRITLK TAFMFPGILF
AIFFVLNTLI WGERSSGAIP FSTMFALVCL WFGISVPLVF IGSYLGHKKP AIEDPVKTNK
IPRQVPEQPW YMKPGFSILI GGILPFGAVF IELFFILTSI WLNQFYYIFG FLFIVFLILI
VTCAEITIVL CYFQLCSEDY NWCWRAYLTS GSSSLYLFLY SVFYFFTKLE ISKLVSGVLY
FGYMIIISYS FFVLTGSIGF YACLWFVRKI YSSVKID
