ID   TMPA_TREPA              Reviewed;         345 AA.
AC   P07643;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Treponemal membrane protein A;
DE   AltName: Full=Antigen TmpA;
DE   AltName: Full=Membrane protein A;
DE   Flags: Precursor;
GN   Name=tmpA; OrderedLocusNames=TP_0768;
OS   Treponema pallidum (strain Nichols).
OC   Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX   NCBI_TaxID=243276;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Nichols;
RX   PubMed=3922944; DOI=10.1128/jb.162.3.1227-1237.1985;
RA   Hansen E.B., Pedersen P.E., Schouls L.M., Severin E., van Embden J.D.A.;
RT   "Genetic characterization and partial sequence determination of a Treponema
RT   pallidum operon expressing two immunogenic membrane proteins in Escherichia
RT   coli.";
RL   J. Bacteriol. 162:1227-1237(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA   McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA   Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA   Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL   Science 281:375-388(1998).
RN   [3]
RP   DIACYLGLYCEROL AT CYS-22, PALMITOYLATION AT CYS-22, AND MUTAGENESIS OF
RP   CYS-22.
RC   STRAIN=Nichols;
RX   PubMed=1894360; DOI=10.1128/iai.59.10.3536-3546.1991;
RA   Schouls L.M., van der Heide H.G.J., van Embden J.D.A.;
RT   "Characterization of the 35-kilodalton Treponema pallidum subsp. pallidum
RT   recombinant lipoprotein TmpC and antibody response to lipidated and
RT   nonlipidated T. pallidum antigens.";
RL   Infect. Immun. 59:3536-3546(1991).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor.
CC   -!- SIMILARITY: To T.phagedenis TmpA. {ECO:0000305}.
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DR   EMBL; M10931; AAA27481.1; -; Genomic_DNA.
DR   EMBL; AE000520; AAC65736.1; -; Genomic_DNA.
DR   PIR; A24587; A24587.
DR   RefSeq; WP_010882213.1; NC_021490.2.
DR   AlphaFoldDB; P07643; -.
DR   SMR; P07643; -.
DR   IntAct; P07643; 2.
DR   STRING; 243276.TPANIC_0768; -.
DR   EnsemblBacteria; AAC65736; AAC65736; TP_0768.
DR   KEGG; tpa:TP_0768; -.
DR   eggNOG; ENOG5031CFT; Bacteria.
DR   HOGENOM; CLU_871359_0_0_12; -.
DR   OMA; FWEASEA; -.
DR   OrthoDB; 1091615at2; -.
DR   Proteomes; UP000000811; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal.
FT   SIGNAL          1..21
FT   CHAIN           22..345
FT                   /note="Treponemal membrane protein A"
FT                   /id="PRO_0000018197"
FT   REGION          256..345
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        297..324
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           22
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000269|PubMed:1894360"
FT   LIPID           22
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000269|PubMed:1894360"
FT   MUTAGEN         22
FT                   /note="C->S: Loss of lipid incorporation and cleavage at
FT                   alternative processing site."
FT                   /evidence="ECO:0000269|PubMed:1894360"
SQ   SEQUENCE   345 AA;  37285 MW;  92CA2331761BEC94 CRC64;
     MNAHTLVYSG VALACAAMLG SCASGAKEEA EKKAAEQRAL LVESAHADRR LMEARIGAQE
     SGADTQHPEL FSQIQDVERQ STDAKIEGDL KKAAGVASEA ADKYEILRNR VEVADLQSKI
     QTHQLAQYDG DSANAAEESW KKALELYETD SAQCLQSTVE ALESYRKVAH EGFGRLLPDM
     KARAGAAKTD VGGLKVAVEL RPQLEEADSQ YQEAREAEEV NARAKAFSGY HRALEIYTEL
     GKVVRLKKTE AEKALQSAKT KQKASSDLAR SADKSAPLPE NAQGFSKEPI EVEPLPNDRL
     NTTQADESAP IPISDTSSPS RVQSRGVEDG GRSPKSSMNE EGASR