ID   TMPS_METJA              Reviewed;         260 AA.
AC   Q58167;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Thymidylate synthase;
DE            Short=TS;
DE            Short=TSase;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=MJ0757;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   FUNCTION AS A THYMIDYLATE SYNTHASE.
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=10426953; DOI=10.1038/11525;
RA   Xu H., Aurora R., Rose G.D., White R.H.;
RT   "Identifying two ancient enzymes in Archaea using predicted secondary
RT   structure alignment.";
RL   Nat. Struct. Biol. 6:750-754(1999).
CC   -!- FUNCTION: Is able to catalyze the biosynthesis of dTMP using dUMP,
CC       tetrahydrofolate and formaldehyde in vitro, i.e. a reaction equivalent
CC       to that catalyzed by bacterial thymidylate synthases (EC 2.1.1.45).
CC       However, M.jannaschii like most methanogenic Archaea lacks folates,
CC       thus the physiological cosubstrate is unknown but is likely one of the
CC       non-methylated methanopterin biosynthetic intermediates.
CC       {ECO:0000269|PubMed:10426953}.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
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DR   EMBL; L77117; AAB98749.1; -; Genomic_DNA.
DR   PIR; E64394; E64394.
DR   AlphaFoldDB; Q58167; -.
DR   STRING; 243232.MJ_0757; -.
DR   PRIDE; Q58167; -.
DR   DNASU; 1451634; -.
DR   EnsemblBacteria; AAB98749; AAB98749; MJ_0757.
DR   KEGG; mja:MJ_0757; -.
DR   eggNOG; arCOG02468; Archaea.
DR   HOGENOM; CLU_077076_0_0_2; -.
DR   OMA; HHSYPCT; -.
DR   PhylomeDB; Q58167; -.
DR   BioCyc; MetaCyc:MON-14571; -.
DR   UniPathway; UPA00575; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0004799; F:thymidylate synthase activity; IDA:UniProtKB.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR003745; DUF166.
DR   Pfam; PF02593; DUF166; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase; Nucleotide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..260
FT                   /note="Thymidylate synthase"
FT                   /id="PRO_0000107016"
SQ   SEQUENCE   260 AA;  30434 MW;  F0BBD83A4143BF08 CRC64;
     MVLNINSFYK NCRGEFMRAV FIYHKNNQRM EKFYKNLLNE PDFCRICDDC YNCRGNWTFK
     NNVKNIVIEE VYEEFVDNPY DYLPELPEGD ICIAQLHEDL LYELPLLLKE KGYKALIVPS
     ETPHDLSLAL RRDLKRVCSN YNIEFENPKP FCSLEKKEGN EYINKFIDYF KIGKPELEIE
     VENGLIKDVK VKISAPCGET YYIAKRLKGK AIDDLKEEIA NAHHNYPCLA SMEMDKELGD
     TILHKAGYIA FEVVEKALKK