ID   TMP_BPT5                Reviewed;        1219 AA.
AC   Q6QGE7;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Tape measure protein pb2 {ECO:0000303|PubMed:18348984};
DE            Short=TMP-pb2 {ECO:0000305};
DE   AltName: Full=Pore-forming protein pb2 {ECO:0000305};
DE   AltName: Full=Tail protein pb2 {ECO:0000303|PubMed:24198424};
DE   Flags: Precursor;
GN   Name=D18-19 {ECO:0000312|EMBL:AAQ92756.2};
GN   ORFNames=T5.140 {ECO:0000312|EMBL:AAQ92756.2},
GN   T5p136 {ECO:0000312|EMBL:AAU05275.1};
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12066.1};
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SUBCELLULAR LOCATION,
RP   FUNCTION, AND PROTEOLYTIC CLEAVAGE.
RC   STRAIN=St0 deletion mutant;
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=18348984; DOI=10.1074/jbc.m800052200;
RA   Boulanger P., Jacquot P., Plancon L., Chami M., Engel A., Parquet C.,
RA   Herbeuval C., Letellier L.;
RT   "Phage T5 straight tail fiber is a multifunctional protein acting as a tape
RT   measure and carrying fusogenic and muralytic activities.";
RL   J. Biol. Chem. 283:13556-13564(2008).
RN   [5]
RP   REVIEW.
RX   PubMed=22991936; DOI=10.3109/1040841x.2012.723675;
RA   Rodriguez-Rubio L., Martinez B., Donovan D.M., Rodriguez A., Garcia P.;
RT   "Bacteriophage virion-associated peptidoglycan hydrolases: potential new
RT   enzybiotics.";
RL   Crit. Rev. Microbiol. 39:427-434(2013).
CC   -!- FUNCTION: Fills the tail tube with its long coiled-coil domain and has
CC       pore-forming properties for host membrane penetration. Probable
CC       exolysin involved in host peptidoglycan digestion necessary for viral
CC       DNA entry into the host cell. Also functions as a tape measure protein
CC       that serves as a base for tail tube protein polymerization and acts as
CC       a template for tail length determination. {ECO:0000269|PubMed:18348984,
CC       ECO:0000269|PubMed:24198424}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:24198424}.
CC       Note=Probably fills the tail tube with its coiled-coil domain and has
CC       its C-terminus within the tail tip. {ECO:0000269|PubMed:24198424}.
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DR   EMBL; AY543070; AAQ92756.2; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05275.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX12066.1; -; Genomic_DNA.
DR   RefSeq; YP_006968.1; NC_005859.1.
DR   SMR; Q6QGE7; -.
DR   PRIDE; Q6QGE7; -.
DR   GeneID; 2777629; -.
DR   KEGG; vg:2777629; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   Proteomes; UP000002503; Genome.
DR   GO; GO:0098015; C:virus tail; IDA:UniProtKB.
DR   GO; GO:0061783; F:peptidoglycan muralytic activity; IDA:CACAO.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0098932; P:disruption by virus of host cell wall peptidoglycan during virus entry; IEA:UniProtKB-KW.
DR   GO; GO:0044409; P:entry into host; IDA:UniProtKB.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0044694; P:pore-mediated entry of viral genome into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR   GO; GO:0098003; P:viral tail assembly; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antimicrobial; Bacteriolytic enzyme; Coiled coil;
KW   Degradation of host cell envelope components during virus entry;
KW   Degradation of host peptidoglycans during virus entry; Late protein;
KW   Pore-mediated penetration of viral genome into host cell;
KW   Reference proteome; Viral genome ejection through host cell envelope;
KW   Viral long flexible tail ejection system;
KW   Viral penetration into host cytoplasm; Viral release from host cell;
KW   Viral tail assembly; Virion; Virus entry into host cell.
FT   CHAIN           1..1148
FT                   /note="Tape measure protein pb2"
FT                   /id="PRO_0000432975"
FT   PROPEP          1149..1219
FT                   /evidence="ECO:0000269|PubMed:24198424"
FT                   /id="PRO_0000435547"
FT   COILED          441..519
FT                   /evidence="ECO:0000255"
FT   COILED          838..941
FT                   /evidence="ECO:0000255"
FT   COILED          1003..1032
FT                   /evidence="ECO:0000255"
FT   SITE            ?1127..?1128
FT                   /note="Cleavage; by the prohead protease"
FT                   /evidence="ECO:0000269|PubMed:24198424"
SQ   SEQUENCE   1219 AA;  131496 MW;  4A47C5F3DB7464D9 CRC64;
     MTDKLIRELL IDVKQKGATR TAKSIENVSD ALENAAAASE LTNEQLGKMP RTLYSIERAA
     DRAAKSLTKM QASRGMAGIT KSIDGIGDKL DYLAIQLIEV TDKLEIGFDG VSRSVKAMGN
     DVAAATEKVQ DRLYDTNRAL GGTSKGFNDT AGAAGRASRA LGNTSGSARG ATRDFAAMAK
     IGGRLPIMYA ALASNVFVLQ TAFESLKVGD QLNRLEQFGT IVGTMTGTPV QTLALSLQNA
     TNGAISFEEA MRQASSASAY GFDSEQLEQF GLVARRAAAV LGVDMTDALN RVIKGVSKQE
     IELLDELGVT IRLNDAYENY VKQLNATSTG IKYTVDSLTT YQKQQAYANE VIAESTRRFG
     YLDDALKATS WEQFAANANS ALRSLQQSAA TYLNPVMDTL NTFLYQTKSS QMRVSAMARS
     ASAKTTPAEN VTALIENAVG AREDLDTYLK ESEERVKKAQ ELKQQLDDLK AKQAATAPIA
     NALTAGGIGG DESNKLVVQL TNELARQNKE IEERTKTEKV LRQAVQDTGE ALLRNGKLAE
     QLGAKMKYAD TAVPGDKGVF EVDPNNLKAV SEIQKNFDFL KKSSSDTANN IRMAASSITN
     AKKASSDLNS VVKAVEDTSK VTGQSADTLV KNLNLGFSSL DQMKAAQKGL SEYVTAMDKS
     EQNALEVAKR KDEVYNQTKD KAKAEAAARE VLLRQQQEQL TAAKALLAIN PNDPEALKQV
     AKIETEILNT KAQGFENAKK TKDYTDKILG VDREIALLND RTMTSTQYRL AQLRLELQLE
     QEKTELYSKQ ADGQAKVEQS RRAQAQISRE IWEAEKQGTA SHVSALMDAL EVSQTQRNVT
     GQSQILTERL SILQQQLELS KGNTEEELKY RNEIYKTSAA LEQLKKQRES QMQQQVGSSV
     GATYTPTTGL IGEDKDFADM QNRMASYDQA ISKLSELNSE ATAVAQSMGN LTNAMIQFSQ
     GSLDTTSMIA SGMQTVASMI QYSTSQQVSA IDQAIAAEQK RDGKSEASKA KLKKLEAEKL
     KIQQDAAKKQ IIIQTAVAVM QAATAVPYPF SIPLMVAAGL AGALALAQAS SASGMSSIAD
     SGADTTQYLT LGERQKNVDV SMQASSGELS YLRGDKGIGN ANSFVPRAEG GMMYPGVSYQ
     MGEHGTEVVT PMVPMKATPN DQLSDGSKTT SGRPIILNIS TMDAASFRDF ASNNSTAFRD
     AVELALNENG TTLKSLGNS