TMP_LAMBD
ID TMP_LAMBD Reviewed; 853 AA.
AC P03736;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 23-FEB-2022, entry version 87.
DE RecName: Full=Tape measure protein {ECO:0000255|HAMAP-Rule:MF_04138};
DE Short=TMP {ECO:0000255|HAMAP-Rule:MF_04138};
DE AltName: Full=Gene product H {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000303|PubMed:23911548};
DE Short=gpH {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000303|PubMed:23911548};
DE AltName: Full=Minor tail protein H {ECO:0000255|HAMAP-Rule:MF_04138};
DE Contains:
DE RecName: Full=Entry protein H* {ECO:0000255|HAMAP-Rule:MF_04138};
GN Name=H {ECO:0000255|HAMAP-Rule:MF_04138}; OrderedLocusNames=lambdap16;
OS Escherichia phage lambda (Bacteriophage lambda).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lambdavirus.
OX NCBI_TaxID=10710;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT "Nucleotide sequence of bacteriophage lambda DNA.";
RL J. Mol. Biol. 162:729-773(1982).
RN [2]
RP FUNCTION.
RX PubMed=1108413; DOI=10.1016/0042-6822(76)90207-5;
RA Scandella D., Arber W.;
RT "Phage lambda DNA injection into Escherichia coli pel- mutants is restored
RT by mutations in phage genes V or H.";
RL Virology 69:206-215(1976).
RN [3]
RP POST-TRANSLATIONAL MODIFICATION, AND FUNCTION.
RX PubMed=6220513; DOI=10.1016/0042-6822(83)90199-x;
RA Tsui L.C., Hendrix R.W.;
RT "Proteolytic processing of phage lambda tail protein gpH: timing of the
RT cleavage.";
RL Virology 125:257-264(1983).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=4476800; DOI=10.1016/0022-2836(74)90500-2;
RA Casjens S.R., Hendrix R.W.;
RT "Locations and amounts of major structural proteins in bacteriophage
RT lambda.";
RL J. Mol. Biol. 88:535-545(1974).
RN [5]
RP FUNCTION.
RX PubMed=6228546; DOI=10.1128/jb.157.1.165-170.1984;
RA Roessner C.A., Ihler G.M.;
RT "Proteinase sensitivity of bacteriophage lambda tail proteins gpJ and pH in
RT complexes with the lambda receptor.";
RL J. Bacteriol. 157:165-170(1984).
RN [6]
RP FUNCTION.
RX PubMed=2150582; DOI=10.1016/0065-227x(90)90004-d;
RA Katsura I.;
RT "Mechanism of length determination in bacteriophage lambda tails.";
RL Adv. Biophys. 26:1-18(1990).
RN [7]
RP INTERACTION WITH TAIL ASSEMBLY PROTEIN G, INTERACTION WITH TAIL ASSEMBLY
RP PROTEIN GT, AND FUNCTION.
RX PubMed=23911548; DOI=10.1016/j.jmb.2013.06.040;
RA Xu J., Hendrix R.W., Duda R.L.;
RT "Chaperone-protein interactions that mediate assembly of the bacteriophage
RT lambda tail to the correct length.";
RL J. Mol. Biol. 426:1004-1018(2014).
CC -!- FUNCTION: Serves as a ruler that controls the length of tail by
CC stopping the tail tube polymerization and is probably released from the
CC tail shaft during infection to facilitate DNA translocation into the
CC host cell (PubMed:2150582). Assembles into a multimeric linear form
CC possibly stabilized by the covering tail assembly proteins G and GT
CC (PubMed:23911548). Its C-terminus fixes the tail tip complex (J, I, L,
CC K), thereby forming the tail assembly initiator complex
CC (PubMed:23911548). Tail tube proteins polymerize around tape measure
CC protein, displacing the tail assembly protein G and GT
CC (PubMed:23911548). When the tail reaches the length specified by the
CC tape measure protein, it stops and becomes capped by the tail
CC terminator protein (Probable). Upon tail assembly, tape measure protein
CC is cleaved into a form called H*, that plays a role later during virion
CC entry in a new cell (Probable). Once assembled, the virion is released
CC and can infect new cell by binding to the entry receptor LambB
CC (Probable). After opening of a pore on the external membrane, the entry
CC protein H* protein is probably released in the periplasmic space for
CC successfull DNA injection (PubMed:23911548). {ECO:0000255|HAMAP-
CC Rule:MF_04138, ECO:0000269|PubMed:2150582, ECO:0000269|PubMed:23911548,
CC ECO:0000303|PubMed:23911548, ECO:0000305|PubMed:1108413,
CC ECO:0000305|PubMed:23911548, ECO:0000305|PubMed:6220513,
CC ECO:0000305|PubMed:6228546}.
CC -!- SUBUNIT: Interacts with the tail initiator complex presumably through
CC its C-terminus domain (Probable). Interacts with the tail assembly
CC protein G (PubMed:23911548). Interacts with the tail assembly protein
CC GT (PubMed:23911548). {ECO:0000255|HAMAP-Rule:MF_04138,
CC ECO:0000269|PubMed:23911548, ECO:0000305|PubMed:23911548}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04138,
CC ECO:0000269|PubMed:4476800}. Note=There are 6 copies of entry protein
CC H* per mature phage. {ECO:0000255|HAMAP-Rule:MF_04138,
CC ECO:0000269|PubMed:4476800}.
CC -!- PTM: Cleaved into mature protein H* by an unidentified protease.
CC {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000269|PubMed:6220513}.
CC -!- SIMILARITY: Belongs to the Lambdavirus tape measure protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04138}.
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DR EMBL; J02459; AAA96548.1; -; Genomic_DNA.
DR PIR; G43008; TLBPHL.
DR RefSeq; NP_040595.1; NC_001416.1.
DR SMR; P03736; -.
DR IntAct; P03736; 4.
DR GeneID; 2703511; -.
DR KEGG; vg:2703511; -.
DR Proteomes; UP000001711; Genome.
DR GO; GO:0098015; C:virus tail; IEA:UniProtKB-UniRule.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR GO; GO:0098003; P:viral tail assembly; IDA:UniProtKB.
DR HAMAP; MF_04138; TMP_LAMBDA; 1.
DR InterPro; IPR043680; GpH_LAMBDA.
DR InterPro; IPR006431; Phage_tape_meas_C.
DR InterPro; IPR009628; Phage_tape_measure_N.
DR Pfam; PF09718; Tape_meas_lam_C; 1.
DR Pfam; PF06791; TMP_2; 1.
DR TIGRFAMs; TIGR01541; tape_meas_lam_C; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Reference proteome;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral release from host cell;
KW Viral tail assembly; Viral tail protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..853
FT /note="Tape measure protein"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04138"
FT /id="PRO_0000077666"
FT CHAIN ?..853
FT /note="Entry protein H*"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04138"
FT /id="PRO_0000223267"
FT REGION 370..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 360..387
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04138"
FT COILED 548..568
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04138"
SQ SEQUENCE 853 AA; 92280 MW; BDC48EC298C2E04F CRC64;
MAEPVGDLVV DLSLDAARFD EQMARVRRHF SGTESDAKKT AAVVEQSLSR QALAAQKAGI
SVGQYKAAMR MLPAQFTDVA TQLAGGQSPW LILLQQGGQV KDSFGGMIPM FRGLAGAITL
PMVGATSLAV ATGALAYAWY QGNSTLSDFN KTLVLSGNQA GLTADRMLVL SRAGQAAGLT
FNQTSESLSA LVKAGVSGEA QIASISQSVA RFSSASGVEV DKVAEAFGKL TTDPTSGLTA
MARQFHNVSA EQIAYVAQLQ RSGDEAGALQ AANEAATKGF DDQTRRLKEN MGTLETWADR
TARAFKSMWD AVLDIGRPDT AQEMLIKAEA AYKKADDIWN LRKDDYFVND EARARYWDDR
EKARLALEAA RKKAEQQTQQ DKNAQQQSDT EASRLKYTEE AQKAYERLQT PLEKYTARQE
ELNKALKDGK ILQADYNTLM AAAKKDYEAT LKKPKQSSVK VSAGDRQEDS AHAALLTLQA
ELRTLEKHAG ANEKISQQRR DLWKAESQFA VLEEAAQRRQ LSAQEKSLLA HKDETLEYKR
QLAALGDKVT YQERLNALAQ QADKFAQQQR AKRAAIDAKS RGLTDRQAER EATEQRLKEQ
YGDNPLALNN VMSEQKKTWA AEDQLRGNWM AGLKSGWSEW EESATDSMSQ VKSAATQTFD
GIAQNMAAML TGSEQNWRSF TRSVLSMMTE ILLKQAMVGI VGSIGSAIGG AVGGGASASG
GTAIQAAAAK FHFATGGFTG TGGKYEPAGI VHRGEFVFTK EATSRIGVGN LYRLMRGYAT
GGYVGTPGSM ADSRSQASGT FEQNNHVVIN NDGTNGQIGP AALKAVYDMA RKGARDEIQT
QMRDGGLFSG GGR
