TMRB_BACSU
ID TMRB_BACSU Reviewed; 197 AA.
AC P12921; P94385;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 4.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Tunicamycin resistance protein;
GN Name=tmrB; OrderedLocusNames=BSU03140;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Harada S., Yoda K., Mori M., Tanimoto A., Furusato T., Yamane K.,
RA Takatsuki A., Yamasaki M., Tamura G.;
RT "The gene responsible for tunicamycin resistance, tmrB, in Bacillus
RT subtilis.";
RL Agric. Biol. Chem. 52:1785-1789(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 33.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP PROTEIN SEQUENCE OF 1-10, AND CHARACTERIZATION.
RX PubMed=1624425; DOI=10.1128/jb.174.13.4302-4307.1992;
RA Noda Y., Yoda K., Takatsuki A., Yamasaki M.;
RT "TmrB protein, responsible for tunicamycin resistance of Bacillus subtilis,
RT is a novel ATP-binding membrane protein.";
RL J. Bacteriol. 174:4302-4307(1992).
CC -!- FUNCTION: Involved in the resistance to tunicamycin. Binds ATP.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA02944.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA02945.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D13793; BAA02946.1; -; Genomic_DNA.
DR EMBL; D13793; BAA02944.1; ALT_INIT; Genomic_DNA.
DR EMBL; D13793; BAA02945.1; ALT_INIT; Genomic_DNA.
DR EMBL; D50453; BAA08948.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12108.2; -; Genomic_DNA.
DR PIR; E69724; YTBST.
DR RefSeq; NP_388196.2; NC_000964.3.
DR RefSeq; WP_003246258.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P12921; -.
DR SMR; P12921; -.
DR STRING; 224308.BSU03140; -.
DR PaxDb; P12921; -.
DR PRIDE; P12921; -.
DR EnsemblBacteria; CAB12108; CAB12108; BSU_03140.
DR GeneID; 938346; -.
DR KEGG; bsu:BSU03140; -.
DR PATRIC; fig|224308.179.peg.328; -.
DR eggNOG; COG1660; Bacteria.
DR InParanoid; P12921; -.
DR OMA; MLLWING; -.
DR PhylomeDB; P12921; -.
DR BioCyc; BSUB:BSU03140-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; ATP-binding; Cell membrane;
KW Direct protein sequencing; Membrane; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..197
FT /note="Tunicamycin resistance protein"
FT /id="PRO_0000072604"
FT BINDING 7..14
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 33
FT /note="E -> Q (in Ref. 1; BAA02946/BAA02944/BAA02945 and 2;
FT BAA08948)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 197 AA; 22730 MW; FBBB871924642EF2 CRC64;
MIIWINGAFG SGKTQTAFEL HRRLNPSYVY DPEKMGFALR SMVPQEIAKD DFQSYPLWRA
FNYSLLASLT DTYRGILIVP MTIVHPEYFN EIIGRLRQEG RIVHHFTLMA SKETLLKRLR
TRAEGKNSWA AKQIDRCVEG LSSPIFEDHI QTDNLSIQDV AENIAARAEL PLDPDTRGSL
RRFADRLMVK LNHIRIK
