TMUB2_MOUSE
ID TMUB2_MOUSE Reviewed; 319 AA.
AC Q3V209; A2AWT1; Q8K0X0; Q91YQ0; Q9D8C5;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Transmembrane and ubiquitin-like domain-containing protein 2;
GN Name=Tmub2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Head, and Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Colon, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP STRUCTURE BY NMR OF 173-254.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of mouse hypothetical ubiquitin-like protein
RT BAB25500.";
RL Submitted (MAY-2004) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3V209-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3V209-2; Sequence=VSP_019705;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH16187.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB25500.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK008158; BAB25500.1; ALT_FRAME; mRNA.
DR EMBL; AK132118; BAE20990.1; -; mRNA.
DR EMBL; AL954730; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC016187; AAH16187.1; ALT_INIT; mRNA.
DR EMBL; BC029841; AAH29841.2; -; mRNA.
DR CCDS; CCDS25494.1; -. [Q3V209-1]
DR RefSeq; NP_001289434.1; NM_001302505.1. [Q3V209-1]
DR RefSeq; NP_001289435.1; NM_001302506.1. [Q3V209-2]
DR RefSeq; NP_082352.1; NM_028076.3. [Q3V209-1]
DR PDB; 1WIA; NMR; -; A=173-254.
DR PDBsum; 1WIA; -.
DR AlphaFoldDB; Q3V209; -.
DR SMR; Q3V209; -.
DR STRING; 10090.ENSMUSP00000116327; -.
DR iPTMnet; Q3V209; -.
DR PhosphoSitePlus; Q3V209; -.
DR EPD; Q3V209; -.
DR MaxQB; Q3V209; -.
DR PaxDb; Q3V209; -.
DR PRIDE; Q3V209; -.
DR ProteomicsDB; 259441; -. [Q3V209-1]
DR ProteomicsDB; 259442; -. [Q3V209-2]
DR Antibodypedia; 29728; 80 antibodies from 16 providers.
DR Ensembl; ENSMUST00000036376; ENSMUSP00000047600; ENSMUSG00000034757. [Q3V209-1]
DR Ensembl; ENSMUST00000156326; ENSMUSP00000116327; ENSMUSG00000034757. [Q3V209-1]
DR GeneID; 72053; -.
DR KEGG; mmu:72053; -.
DR UCSC; uc007lrb.2; mouse. [Q3V209-1]
DR CTD; 79089; -.
DR MGI; MGI:1919303; Tmub2.
DR VEuPathDB; HostDB:ENSMUSG00000034757; -.
DR eggNOG; ENOG502QU8U; Eukaryota.
DR GeneTree; ENSGT00390000014069; -.
DR HOGENOM; CLU_053940_0_0_1; -.
DR InParanoid; Q3V209; -.
DR OMA; QMKFIYQ; -.
DR OrthoDB; 1164744at2759; -.
DR TreeFam; TF329265; -.
DR BioGRID-ORCS; 72053; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Tmub2; mouse.
DR EvolutionaryTrace; Q3V209; -.
DR PRO; PR:Q3V209; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q3V209; protein.
DR Bgee; ENSMUSG00000034757; Expressed in granulocyte and 262 other tissues.
DR ExpressionAtlas; Q3V209; baseline and differential.
DR Genevisible; Q3V209; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR InterPro; IPR040352; TMUB1/2.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR14557; PTHR14557; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..319
FT /note="Transmembrane and ubiquitin-like domain-containing
FT protein 2"
FT /id="PRO_0000245313"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 173..246
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT REGION 88..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..115
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..20
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019705"
FT CONFLICT 65
FT /note="Q -> E (in Ref. 3; AAH16187)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="L -> H (in Ref. 3; AAH29841)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="W -> R (in Ref. 1; BAB25500)"
FT /evidence="ECO:0000305"
FT STRAND 173..179
FT /evidence="ECO:0007829|PDB:1WIA"
FT TURN 180..182
FT /evidence="ECO:0007829|PDB:1WIA"
FT STRAND 183..189
FT /evidence="ECO:0007829|PDB:1WIA"
FT STRAND 191..194
FT /evidence="ECO:0007829|PDB:1WIA"
FT HELIX 195..202
FT /evidence="ECO:0007829|PDB:1WIA"
FT STRAND 203..205
FT /evidence="ECO:0007829|PDB:1WIA"
FT TURN 206..209
FT /evidence="ECO:0007829|PDB:1WIA"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:1WIA"
FT TURN 226..230
FT /evidence="ECO:0007829|PDB:1WIA"
FT STRAND 235..240
FT /evidence="ECO:0007829|PDB:1WIA"
SQ SEQUENCE 319 AA; 33808 MW; 51CBF8B414E1CAD8 CRC64;
MISRLLQNNL MSVDPVSSQA MELSDVTLIE GVGNEVMVVA GVVALTLALV LAWLSTYVAD
SGNNQLLGTI VSAGDTSVLH LGHVDQLVNQ GTPEPTEHPH PSGGNDDKAE ETSDSGGDAT
GEPGARGEME PSLEHLLDIQ GLPKRQAGLG SSRPEAPLGL DDGSCLSPSP SLINVRLKFL
NDTEELAVAR PEDTVGTLKS KYFPGQESQM KLIYQGRLLQ DPARTLSSLN ITNNCVIHCH
RSPPGAAVSG PSASLTPTTE QSSLGVNVGS LMVPVFVVLL GVVWYFRINY RQFFTGPATI
SLVGVTVFFS ILVFGMYGR
