TMX4_MOUSE
ID TMX4_MOUSE Reviewed; 335 AA.
AC Q8C0L0; Q3UHC6; Q6ZPW7; Q80X49;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Thioredoxin-related transmembrane protein 4;
DE AltName: Full=Thioredoxin domain-containing protein 13;
DE Flags: Precursor;
GN Name=Tmx4; Synonyms=D2Bwg1356e, Kiaa1162, Txndc13;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=31142202; DOI=10.1080/19491034.2019.1618175;
RA Cheng L.C., Baboo S., Lindsay C., Brusman L., Martinez-Bartolome S.,
RA Tapia O., Zhang X., Yates J.R. III, Gerace L.;
RT "Identification of new transmembrane proteins concentrated at the nuclear
RT envelope using organellar proteomics of mesenchymal cells.";
RL Nucleus 10:126-143(2019).
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000269|PubMed:31142202}; Single-pass type I membrane protein
CC {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH50918.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC98111.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK030696; BAC27085.1; -; mRNA.
DR EMBL; AK129301; BAC98111.1; ALT_INIT; mRNA.
DR EMBL; AK147465; BAE27931.1; -; mRNA.
DR EMBL; BC050918; AAH50918.1; ALT_INIT; mRNA.
DR CCDS; CCDS16784.1; -.
DR RefSeq; NP_083424.1; NM_029148.1.
DR AlphaFoldDB; Q8C0L0; -.
DR SMR; Q8C0L0; -.
DR BioGRID; 206848; 5.
DR STRING; 10090.ENSMUSP00000045154; -.
DR iPTMnet; Q8C0L0; -.
DR PhosphoSitePlus; Q8C0L0; -.
DR SwissPalm; Q8C0L0; -.
DR EPD; Q8C0L0; -.
DR MaxQB; Q8C0L0; -.
DR PaxDb; Q8C0L0; -.
DR PeptideAtlas; Q8C0L0; -.
DR PRIDE; Q8C0L0; -.
DR ProteomicsDB; 259444; -.
DR Antibodypedia; 610; 105 antibodies from 25 providers.
DR Ensembl; ENSMUST00000038228; ENSMUSP00000045154; ENSMUSG00000034723.
DR GeneID; 52837; -.
DR KEGG; mmu:52837; -.
DR UCSC; uc008mnt.2; mouse.
DR CTD; 56255; -.
DR MGI; MGI:106558; Tmx4.
DR VEuPathDB; HostDB:ENSMUSG00000034723; -.
DR eggNOG; KOG0913; Eukaryota.
DR GeneTree; ENSGT00940000160301; -.
DR HOGENOM; CLU_069292_1_0_1; -.
DR InParanoid; Q8C0L0; -.
DR OMA; YIVEKKW; -.
DR OrthoDB; 1481386at2759; -.
DR PhylomeDB; Q8C0L0; -.
DR TreeFam; TF106376; -.
DR BioGRID-ORCS; 52837; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Tmx4; mouse.
DR PRO; PR:Q8C0L0; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8C0L0; protein.
DR Bgee; ENSMUSG00000034723; Expressed in medial preoptic region and 217 other tissues.
DR ExpressionAtlas; Q8C0L0; baseline and differential.
DR Genevisible; Q8C0L0; MM.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005637; C:nuclear inner membrane; IDA:UniProtKB.
DR GO; GO:0015036; F:disulfide oxidoreductase activity; IBA:GO_Central.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR017937; Thioredoxin_CS.
DR InterPro; IPR013766; Thioredoxin_domain.
DR Pfam; PF00085; Thioredoxin; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS00194; THIOREDOXIN_1; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Electron transport; Membrane; Nucleus; Phosphoprotein;
KW Redox-active center; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..335
FT /note="Thioredoxin-related transmembrane protein 4"
FT /id="PRO_0000034192"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 26..133
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT REGION 222..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..282
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..316
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 247
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H1E5"
FT MOD_RES 255
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H1E5"
FT DISULFID 60..63
FT /note="Redox-active"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT CONFLICT 22
FT /note="G -> S (in Ref. 1; BAC27085)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 37131 MW; 526E4E6F894737D2 CRC64;
MTGGFCVPVL LAAWLAAAAA EGLEQAALPA EESRVQPMTA SNWTLVMEGE WMLKFYAPWC
PSCQQTDSEW ETFAKNGETL QISVGKVDVI QEPGLSGRFF VTTLPAFFHA KDGIFRRYRG
PGIYEDLQNY ILEKKWQSVE PLTGWKSPAS LTMSGMAGLF SISGKIWHLH NYFTVTLGIP
AWCSYVFFVI ATLVFGLFMG LILVVISECF CVPLPRASSE RCEQEQSTGE AQGAEQLQDA
EEEKDDSNEE ENKDSLVDDE EEKEDIGDED EGEEDEEEDN LAGIMAEERS DTNERAVVKE
GSVSPKEDGA HPADTQDVVE DALRQRKSQN ANKGS
