ID   BTDA_PAPHA              Reviewed;           9 AA.
AC   P86722;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Theta defensin subunit A {ECO:0000250|UniProtKB:B6ULW4};
OS   Papio hamadryas (Hamadryas baboon).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Papio.
OX   NCBI_TaxID=9557;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, AND MASS SPECTROMETRY.
RC   TISSUE=Leukocyte {ECO:0000269|PubMed:20155756};
RX   PubMed=20155756; DOI=10.1002/rcm.4424;
RA   Stegemann C., Tsvetkova E.V., Aleshina G.M., Lehrer R.I., Kokryakov V.N.,
RA   Hoffmann R.;
RT   "De novo sequencing of two new cyclic theta-defensins from baboon (Papio
RT   hamadryas) leukocytes by matrix-assisted laser desorption/ionization mass
RT   spectrometry.";
RL   Rapid Commun. Mass Spectrom. 24:599-604(2010).
CC   -!- FUNCTION: PhTD-1 and PhTD-3 have antimicrobial activity. In low salt
CC       conditions PhTD-1 has antibacterial activity against the Gram-negative
CC       bacterium E.coli ML35p (MIC=1.5 uM), the Gram-positive bacteria
CC       L.monocytogenes EGD (MIC=1.6 uM) and methicillin-resistant S.aureus
CC       ATCC 33591 (MIC=2.5 uM), and the fungus C.albicans 820 (MIC=1.6 uM). At
CC       high physiological salt concentrations the antimicrobial activity of
CC       PhTD-1 decreases slightly: E.coli ML35p (MIC=2.0 uM), L.monocytogenes
CC       EGD (MIC=1.7 uM), S.aureus ATCC 33591 (MIC=3.6 uM), and C.albicans 820
CC       (MIC=6.5 uM). In low salt conditions PhTD-3 has antibacterial activity
CC       against E.coli ML35p (MIC=1.7 uM), L.monocytogenes EGD (MIC=1.5 uM),
CC       S.aureus ATCC 33591 (MIC=1.7 uM), and C.albicans 820 (MIC=1.4 uM). At
CC       high physiological salt concentrations the antimicrobial activity of
CC       PhTD-3 decreases slightly: E.coli ML35p (MIC=2.2 uM), L.monocytogenes
CC       EGD (MIC=1.9 uM), S.aureus ATCC 33591 (MIC=4.5 uM), and C.albicans 820
CC       (MIC=7.4 uM). {ECO:0000269|PubMed:20155756}.
CC   -!- SUBUNIT: PhTD-1 is a cyclic heterodimer composed of subunits A and B;
CC       disulfide-linked. PhTD-3 is a cyclic homodimer composed of two subunits
CC       A; disulfide-linked. {ECO:0000269|PubMed:20155756}.
CC   -!- PTM: Forms a cyclic peptide with subunit A (PhTD-3) or with subunit B
CC       (PhTD-1). An additional intersubunit disulfide bond is formed.
CC       {ECO:0000250|UniProtKB:P82270, ECO:0000269|PubMed:20155756}.
CC   -!- MASS SPECTROMETRY: Mass=2051.9; Method=MALDI; Note=PhTD-1, heterodimer,
CC       cyclized.; Evidence={ECO:0000269|PubMed:20155756};
CC   -!- MASS SPECTROMETRY: Mass=2044.9; Method=MALDI; Note=PhTD-3, homodimer,
CC       cyclized.; Evidence={ECO:0000269|PubMed:20155756};
CC   -!- SIMILARITY: Belongs to the alpha-defensin family. Theta subfamily.
CC       {ECO:0000255}.
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DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Fungicide.
FT   PEPTIDE         1..9
FT                   /note="Theta defensin subunit A"
FT                   /evidence="ECO:0000269|PubMed:20155756"
FT                   /id="PRO_0000399460"
FT   DISULFID        2
FT                   /note="Interchain (with C-2 in subunit A); in form PhTD-3"
FT                   /evidence="ECO:0000250|UniProtKB:P82270"
FT   DISULFID        2
FT                   /note="Interchain (with C-2 in subunit B); in form PhTD-1"
FT                   /evidence="ECO:0000250|UniProtKB:P82270"
FT   DISULFID        4..9
FT                   /evidence="ECO:0000250|UniProtKB:P82270"
FT   CROSSLNK        1
FT                   /note="Cyclopeptide (Arg-Cys) (interchain with C-9 in
FT                   subunit A); in form PhTD-3"
FT   CROSSLNK        1
FT                   /note="Cyclopeptide (Arg-Cys) (interchain with C-9 in
FT                   subunit B); in form PhTD-1"
FT   CROSSLNK        9
FT                   /note="Cyclopeptide (Cys-Arg) (interchain with R-1 in
FT                   subunit A); in form PhTD-3"
FT   CROSSLNK        9
FT                   /note="Cyclopeptide (Cys-Arg) (interchain with R-1 in
FT                   subunit B); in form PhTD-1"
SQ   SEQUENCE   9 AA;  1044 MW;  8623C86411AEA2CE CRC64;
     RCVCTRGFC