ID   TNFA_SPAAU              Reviewed;         253 AA.
AC   Q8JFG3;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Tumor necrosis factor;
DE   AltName: Full=TNF-alpha;
GN   Name=tnf; Synonyms=tnfa;
OS   Sparus aurata (Gilthead sea bream).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Spariformes; Sparidae; Sparus.
OX   NCBI_TaxID=8175;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Kidney;
RX   PubMed=12073149; DOI=10.1007/s00251-002-0451-y;
RA   Garcia-Castillo J., Pelegrin P., Mulero V., Meseguer J.;
RT   "Molecular cloning and expression analysis of tumor necrosis factor alpha
RT   from a marine fish reveal its constitutive expression and ubiquitous
RT   nature.";
RL   Immunogenetics 54:200-207(2002).
CC   -!- FUNCTION: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
CC   -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR   EMBL; AJ413189; CAC88353.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8JFG3; -.
DR   SMR; Q8JFG3; -.
DR   Proteomes; UP000472265; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   CDD; cd00184; TNF; 1.
DR   Gene3D; 2.60.120.40; -; 1.
DR   InterPro; IPR006053; TNF.
DR   InterPro; IPR006052; TNF_dom.
DR   InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR   Pfam; PF00229; TNF; 1.
DR   PRINTS; PR01234; TNECROSISFCT.
DR   SMART; SM00207; TNF; 1.
DR   SUPFAM; SSF49842; SSF49842; 1.
DR   PROSITE; PS50049; TNF_2; 1.
PE   3: Inferred from homology;
KW   Cytokine; Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..253
FT                   /note="Tumor necrosis factor"
FT                   /id="PRO_0000185485"
FT   TOPO_DOM        1..34
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..57
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        58..253
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   253 AA;  27978 MW;  730E2A52900B743A CRC64;
     MGAYTTAPCD LEMGPEERTV VLIEKKSSTG WMWKVSVALL IAALCFAGVL LFAWYWNGKP
     EILIHSGQSE ALTKKDHAEK TDPHSTLKRI SSKAKAAIHL EGSYDEDEGL KDQVEWKNGQ
     GQAFAQGGFR LVDNKIVIPH TGLYFVYSQA SFRVSCSDGD EEGAGRHLTP LSHRISRYSE
     SMGSDVSLMS AVRSACQNTA QEDSYSDGRG WYNTIYLGAV FQLNRGDKLE TETNQLSELE
     TDEGKTFFGV FAL