ID   TNFB_BOVIN              Reviewed;         204 AA.
AC   Q06600; A4FUB6;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Lymphotoxin-alpha;
DE            Short=LT-alpha;
DE   AltName: Full=TNF-beta;
DE   AltName: Full=Tumor necrosis factor ligand superfamily member 1;
DE   Flags: Precursor;
GN   Name=LTA; Synonyms=TNFB, TNFSF1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8260599; DOI=10.1016/1043-4666(93)90065-d;
RA   Cludts I., Cleuter Y., Kettmann R., Burny A., Droogmans L.;
RT   "Cloning and characterization of the tandemly arranged bovine lymphotoxin
RT   and tumour necrosis factor-alpha genes.";
RL   Cytokine 5:336-341(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Uterus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cytokine that in its homotrimeric form binds to
CC       TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In
CC       its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is
CC       produced by lymphocytes and is cytotoxic for a wide range of tumor
CC       cells in vitro and in vivo. {ECO:0000250|UniProtKB:P01374}.
CC   -!- SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA
CC       subunits or (less prevalent) two LTA and one LTB subunits. Interacts
CC       with TNFRSF14. {ECO:0000250|UniProtKB:P01374}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Membrane {ECO:0000250}.
CC       Note=The homotrimer is secreted. The heterotrimer is membrane-
CC       associated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR   EMBL; Z14137; CAA78510.1; -; Genomic_DNA.
DR   EMBL; BC114670; AAI14671.1; -; mRNA.
DR   PIR; I46046; S24641.
DR   RefSeq; NP_001013419.1; NM_001013401.2.
DR   RefSeq; XP_005223643.1; XM_005223586.3.
DR   AlphaFoldDB; Q06600; -.
DR   SMR; Q06600; -.
DR   STRING; 9913.ENSBTAP00000000018; -.
DR   PaxDb; Q06600; -.
DR   Ensembl; ENSBTAT00000000018; ENSBTAP00000000018; ENSBTAG00000000016.
DR   GeneID; 280845; -.
DR   KEGG; bta:280845; -.
DR   CTD; 4049; -.
DR   VEuPathDB; HostDB:ENSBTAG00000000016; -.
DR   VGNC; VGNC:31066; LTA.
DR   eggNOG; ENOG502S4K8; Eukaryota.
DR   GeneTree; ENSGT01050000244878; -.
DR   HOGENOM; CLU_070352_4_0_1; -.
DR   InParanoid; Q06600; -.
DR   OMA; MEGECKV; -.
DR   OrthoDB; 1124938at2759; -.
DR   TreeFam; TF332169; -.
DR   Reactome; R-BTA-5668541; TNFR2 non-canonical NF-kB pathway.
DR   Reactome; R-BTA-5676594; TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.
DR   Proteomes; UP000009136; Chromosome 23.
DR   Bgee; ENSBTAG00000000016; Expressed in pharyngeal tonsil and 61 other tissues.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:Ensembl.
DR   GO; GO:0006959; P:humoral immune response; IEA:Ensembl.
DR   GO; GO:0048535; P:lymph node development; IEA:Ensembl.
DR   GO; GO:0002876; P:positive regulation of chronic inflammatory response to antigenic stimulus; IEA:Ensembl.
DR   GO; GO:0002925; P:positive regulation of humoral immune response mediated by circulating immunoglobulin; IEA:Ensembl.
DR   GO; GO:0032729; P:positive regulation of interferon-gamma production; IEA:Ensembl.
DR   CDD; cd00184; TNF; 1.
DR   Gene3D; 2.60.120.40; -; 1.
DR   InterPro; IPR006053; TNF.
DR   InterPro; IPR002960; TNF_beta.
DR   InterPro; IPR021184; TNF_CS.
DR   InterPro; IPR006052; TNF_dom.
DR   InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR   Pfam; PF00229; TNF; 1.
DR   PRINTS; PR01234; TNECROSISFCT.
DR   PRINTS; PR01236; TNFBETA.
DR   SMART; SM00207; TNF; 1.
DR   SUPFAM; SSF49842; SSF49842; 1.
DR   PROSITE; PS00251; TNF_1; 1.
DR   PROSITE; PS50049; TNF_2; 1.
PE   2: Evidence at transcript level;
KW   Cytokine; Glycoprotein; Membrane; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000250"
FT   CHAIN           34..204
FT                   /note="Lymphotoxin-alpha"
FT                   /id="PRO_0000034461"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   204 AA;  22099 MW;  E2A9F0A2CEDB5425 CRC64;
     MTPPGRLYLL RVCSTPPLLL LGLLLALPLE AQGLRGIGLT PSAAQPAHQQ LPTPFTRGTL
     KPAAHLVGDP STQDSLRWRA NTDRAFLRHG FSLSNNSLLV PTSGLYFVYS QVVFSGRGCF
     PRATPTPLYL AHEVQLFSPQ YPFHVPLLSA QKSVCPGPQG PWVRSVYQGA VFLLTRGDQL
     STHTDGISHL LLSPSSVFFG AFAL