ID   TNFB_MARMO              Reviewed;         205 AA.
AC   Q9JM09; Q9JM12;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Lymphotoxin-alpha;
DE            Short=LT-alpha;
DE   AltName: Full=TNF-beta;
DE   AltName: Full=Tumor necrosis factor ligand superfamily member 1;
DE   Flags: Precursor;
GN   Name=LTA; Synonyms=TNFB, TNFSF1;
OS   Marmota monax (Woodchuck).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Sciuromorpha; Sciuridae;
OC   Xerinae; Marmotini; Marmota.
OX   NCBI_TaxID=9995;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   TISSUE=Blood;
RX   PubMed=10721723; DOI=10.1016/s0378-1119(99)00494-1;
RA   Li D.H., Havell E.A., Brown C.L., Cullen J.M.;
RT   "Woodchuck lymphotoxin-alpha, -beta and tumor necrosis factor genes:
RT   structure, characterization and biological activity.";
RL   Gene 242:295-305(2000).
CC   -!- FUNCTION: Cytokine that in its homotrimeric form binds to
CC       TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In
CC       its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is
CC       produced by lymphocytes and is cytotoxic for a wide range of tumor
CC       cells in vitro and in vivo. {ECO:0000250|UniProtKB:P01374}.
CC   -!- SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA
CC       subunits or (less prevalent) two LTA and one LTB subunits. Interacts
CC       with TNFRSF14. {ECO:0000250|UniProtKB:P01374}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Membrane {ECO:0000250}.
CC       Note=The homotrimer is secreted. The heterotrimer is membrane-
CC       associated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR   EMBL; AF096268; AAF34868.1; -; Genomic_DNA.
DR   EMBL; AF095586; AAF34864.1; -; mRNA.
DR   AlphaFoldDB; Q9JM09; -.
DR   SMR; Q9JM09; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   CDD; cd00184; TNF; 1.
DR   Gene3D; 2.60.120.40; -; 1.
DR   InterPro; IPR006053; TNF.
DR   InterPro; IPR002960; TNF_beta.
DR   InterPro; IPR021184; TNF_CS.
DR   InterPro; IPR006052; TNF_dom.
DR   InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR   Pfam; PF00229; TNF; 1.
DR   PRINTS; PR01234; TNECROSISFCT.
DR   PRINTS; PR01236; TNFBETA.
DR   SMART; SM00207; TNF; 1.
DR   SUPFAM; SSF49842; SSF49842; 1.
DR   PROSITE; PS00251; TNF_1; 1.
DR   PROSITE; PS50049; TNF_2; 1.
PE   2: Evidence at transcript level;
KW   Cytokine; Glycoprotein; Membrane; Secreted; Signal.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000250"
FT   CHAIN           35..205
FT                   /note="Lymphotoxin-alpha"
FT                   /id="PRO_0000034466"
FT   CARBOHYD        96
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        46
FT                   /note="R -> W (in Ref. 1; AAF34864)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   205 AA;  22229 MW;  EE1F8D8E4C04C8A7 CRC64;
     MTPPGRLYLP RVRGTRLLFL LLGLLLALPP RAKGLPGVGL LPSAARAAQQ HPQKHFAHGT
     LKPAAHLVGD PSMQNSLRWR ANTDRAFLRH GFSLSNNSLL VPTSGLYFVY SQVVFSGEGC
     SSKAVSTPLY LAHEVQLFSS QYPFHVPLLS AQKSVCPGPQ GPWVRSVYQG AVFLLTRGDQ
     LSTHTDGISH LLFSPSSVFF GAFAL