ID   TNFB_NOTEU              Reviewed;         201 AA.
AC   Q9XT48; Q3T4H4;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Lymphotoxin-alpha;
DE            Short=LT-alpha;
DE   AltName: Full=TNF-beta;
DE   AltName: Full=Tumor necrosis factor ligand superfamily member 1;
DE   Flags: Precursor;
GN   Name=LTA; Synonyms=TNFB, TNFSF1;
OS   Notamacropus eugenii (Tammar wallaby) (Macropus eugenii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Metatheria; Diprotodontia; Macropodidae; Notamacropus.
OX   NCBI_TaxID=9315;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10826697; DOI=10.3109/10425170009015608;
RA   Harrison G.A., Deane E.M.;
RT   "cDNA cloning of lymphotoxin alpha (LT-alpha) from a marsupial, Macropus
RT   eugenii.";
RL   DNA Seq. 10:399-403(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16103651; DOI=10.1159/000086379;
RA   Cross J.G., Harrison G.A., Coggill P., Sims S., Beck S., Deakin J.E.,
RA   Graves J.A.;
RT   "Analysis of the genomic region containing the tammar wallaby (Macropus
RT   eugenii) orthologues of MHC class III genes.";
RL   Cytogenet. Genome Res. 111:110-117(2005).
CC   -!- FUNCTION: Cytokine that in its homotrimeric form binds to
CC       TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In
CC       its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is
CC       produced by lymphocytes and is cytotoxic for a wide range of tumor
CC       cells in vitro and in vivo. {ECO:0000250|UniProtKB:P01374}.
CC   -!- SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA
CC       subunits or (less prevalent) two LTA and one LTB subunits. Interacts
CC       with TNFRSF14. {ECO:0000250|UniProtKB:P01374}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Membrane {ECO:0000250}.
CC       Note=The homotrimer is secreted. The heterotrimer is membrane-
CC       associated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR   EMBL; AF119336; AAD41773.1; -; mRNA.
DR   EMBL; AY853666; AAZ99794.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9XT48; -.
DR   SMR; Q9XT48; -.
DR   Ensembl; ENSMEUT00000008309; ENSMEUP00000007573; ENSMEUG00000008296.
DR   HOGENOM; CLU_070352_4_0_1; -.
DR   OMA; RSACQNV; -.
DR   TreeFam; TF332169; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:Ensembl.
DR   GO; GO:0006959; P:humoral immune response; IEA:Ensembl.
DR   GO; GO:0048535; P:lymph node development; IEA:Ensembl.
DR   GO; GO:0002876; P:positive regulation of chronic inflammatory response to antigenic stimulus; IEA:Ensembl.
DR   GO; GO:0002925; P:positive regulation of humoral immune response mediated by circulating immunoglobulin; IEA:Ensembl.
DR   GO; GO:0032729; P:positive regulation of interferon-gamma production; IEA:Ensembl.
DR   CDD; cd00184; TNF; 1.
DR   Gene3D; 2.60.120.40; -; 1.
DR   InterPro; IPR006053; TNF.
DR   InterPro; IPR002960; TNF_beta.
DR   InterPro; IPR021184; TNF_CS.
DR   InterPro; IPR006052; TNF_dom.
DR   InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR   Pfam; PF00229; TNF; 1.
DR   PRINTS; PR01234; TNECROSISFCT.
DR   PRINTS; PR01236; TNFBETA.
DR   SMART; SM00207; TNF; 1.
DR   SUPFAM; SSF49842; SSF49842; 1.
DR   PROSITE; PS00251; TNF_1; 1.
DR   PROSITE; PS50049; TNF_2; 1.
PE   2: Evidence at transcript level;
KW   Cytokine; Glycoprotein; Membrane; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000250"
FT   CHAIN           28..201
FT                   /note="Lymphotoxin-alpha"
FT                   /id="PRO_0000034464"
FT   REGION          23..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        27..51
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        93
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   201 AA;  21536 MW;  8C4C371CB5091627 CRC64;
     MTSSGVLCLL GALSLQVLLL QPPGAQGAPN PDNSHSSSPA PPQTAQHLSQ KSLKRETLKP
     AAHLVGDPSV QDSIHWRANT DHAFLRHGFS LSNNSLLVPT SGLYFVYSQV VFSGASCSEI
     TPTLLYLSHE VLLFSSKYQV HVPLLSAQKS VCSGTQGPWM RSVYQGAVFL LTQGDRLSTY
     TDGVSHLLQS PSSVFFGAFA L