TNFL4_MOUSE
ID TNFL4_MOUSE Reviewed; 198 AA.
AC P43488;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Tumor necrosis factor ligand superfamily member 4;
DE AltName: Full=OX40 ligand;
DE Short=OX40L;
DE AltName: CD_antigen=CD252;
GN Name=Tnfsf4; Synonyms=Ox40l, Txgp1l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8076595; DOI=10.1002/j.1460-2075.1994.tb06715.x;
RA Baum P.R., Gayle R.B. III, Ramsdell F., Srinivasan S., Sorensen R.A.,
RA Watson M.L., Seldin M.F., Baker E., Sutherland G.R., Clifford K.N.,
RA Alderson M.R., Goodwin R.G., Fanslow W.C.;
RT "Molecular characterization of murine and human OX40/OX40 ligand systems:
RT identification of a human OX40 ligand as the HTLV-1-regulated protein
RT gp34.";
RL EMBO J. 13:3992-4001(1994).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 50-198, DISULFIDE BONDS, SUBUNIT,
RP AND GLYCOSYLATION AT ASN-91.
RX PubMed=16905106; DOI=10.1016/j.str.2006.06.015;
RA Compaan D.M., Hymowitz S.G.;
RT "The crystal structure of the costimulatory OX40-OX40L complex.";
RL Structure 14:1321-1330(2006).
CC -!- FUNCTION: Cytokine that binds to TNFRSF4. Co-stimulates T-cell
CC proliferation and cytokine production.
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:16905106}.
CC -!- INTERACTION:
CC P43488; P23510: TNFSF4; Xeno; NbExp=2; IntAct=EBI-519690, EBI-11724451;
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
CC -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR EMBL; U12763; AAA21871.1; -; mRNA.
DR CCDS; CCDS15416.1; -.
DR PIR; S48290; S48290.
DR RefSeq; NP_033478.1; NM_009452.2.
DR PDB; 2HEW; X-ray; 1.45 A; F=51-198.
DR PDB; 2HEY; X-ray; 2.00 A; F/G=51-198.
DR PDBsum; 2HEW; -.
DR PDBsum; 2HEY; -.
DR AlphaFoldDB; P43488; -.
DR SMR; P43488; -.
DR DIP; DIP-6238N; -.
DR IntAct; P43488; 2.
DR STRING; 10090.ENSMUSP00000028024; -.
DR GlyGen; P43488; 1 site.
DR iPTMnet; P43488; -.
DR PhosphoSitePlus; P43488; -.
DR PaxDb; P43488; -.
DR PRIDE; P43488; -.
DR ProteomicsDB; 258791; -.
DR Antibodypedia; 34389; 679 antibodies from 39 providers.
DR DNASU; 22164; -.
DR Ensembl; ENSMUST00000028024; ENSMUSP00000028024; ENSMUSG00000026700.
DR GeneID; 22164; -.
DR KEGG; mmu:22164; -.
DR UCSC; uc007dfn.2; mouse.
DR CTD; 7292; -.
DR MGI; MGI:104511; Tnfsf4.
DR VEuPathDB; HostDB:ENSMUSG00000026700; -.
DR eggNOG; ENOG502ST4X; Eukaryota.
DR GeneTree; ENSGT00390000015127; -.
DR HOGENOM; CLU_091735_0_0_1; -.
DR InParanoid; P43488; -.
DR OMA; NTSCEDF; -.
DR OrthoDB; 1590205at2759; -.
DR PhylomeDB; P43488; -.
DR TreeFam; TF336384; -.
DR Reactome; R-MMU-5669034; TNFs bind their physiological receptors.
DR BioGRID-ORCS; 22164; 0 hits in 112 CRISPR screens.
DR EvolutionaryTrace; P43488; -.
DR PRO; PR:P43488; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P43488; protein.
DR Bgee; ENSMUSG00000026700; Expressed in scapula and 27 other tissues.
DR ExpressionAtlas; P43488; baseline and differential.
DR Genevisible; P43488; MM.
DR GO; GO:0009986; C:cell surface; ISS:BHF-UCL.
DR GO; GO:0005615; C:extracellular space; ISS:BHF-UCL.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IPI:MGI.
DR GO; GO:0032813; F:tumor necrosis factor receptor superfamily binding; IPI:BHF-UCL.
DR GO; GO:0002526; P:acute inflammatory response; IMP:BHF-UCL.
DR GO; GO:0001568; P:blood vessel development; NAS:BHF-UCL.
DR GO; GO:0035783; P:CD4-positive, alpha-beta T cell costimulation; IDA:BHF-UCL.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; ISS:BHF-UCL.
DR GO; GO:0035714; P:cellular response to nitrogen dioxide; IMP:BHF-UCL.
DR GO; GO:0071380; P:cellular response to prostaglandin E stimulus; ISS:BHF-UCL.
DR GO; GO:0008203; P:cholesterol metabolic process; IMP:MGI.
DR GO; GO:0002215; P:defense response to nematode; IMP:BHF-UCL.
DR GO; GO:0006954; P:inflammatory response; IPI:MGI.
DR GO; GO:0045087; P:innate immune response; NAS:BHF-UCL.
DR GO; GO:0035709; P:memory T cell activation; IMP:BHF-UCL.
DR GO; GO:0070236; P:negative regulation of activation-induced cell death of T cells; IC:BHF-UCL.
DR GO; GO:0001818; P:negative regulation of cytokine production; IPI:MGI.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:BHF-UCL.
DR GO; GO:2001237; P:negative regulation of extrinsic apoptotic signaling pathway; IC:BHF-UCL.
DR GO; GO:0032689; P:negative regulation of interferon-gamma production; IDA:BHF-UCL.
DR GO; GO:0032700; P:negative regulation of interleukin-17 production; ISS:BHF-UCL.
DR GO; GO:0045590; P:negative regulation of regulatory T cell differentiation; IMP:BHF-UCL.
DR GO; GO:0070233; P:negative regulation of T cell apoptotic process; IC:BHF-UCL.
DR GO; GO:0045626; P:negative regulation of T-helper 1 cell differentiation; IDA:BHF-UCL.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:BHF-UCL.
DR GO; GO:0042104; P:positive regulation of activated T cell proliferation; ISO:MGI.
DR GO; GO:0046641; P:positive regulation of alpha-beta T cell proliferation; IMP:BHF-UCL.
DR GO; GO:0050871; P:positive regulation of B cell activation; IDA:BHF-UCL.
DR GO; GO:1900281; P:positive regulation of CD4-positive, alpha-beta T cell costimulation; IDA:BHF-UCL.
DR GO; GO:0043372; P:positive regulation of CD4-positive, alpha-beta T cell differentiation; IDA:BHF-UCL.
DR GO; GO:0032722; P:positive regulation of chemokine production; IMP:BHF-UCL.
DR GO; GO:0001819; P:positive regulation of cytokine production; IBA:GO_Central.
DR GO; GO:0002699; P:positive regulation of immune effector process; TAS:BHF-UCL.
DR GO; GO:0002891; P:positive regulation of immunoglobulin mediated immune response; IMP:BHF-UCL.
DR GO; GO:0002639; P:positive regulation of immunoglobulin production; IDA:BHF-UCL.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:BHF-UCL.
DR GO; GO:0032729; P:positive regulation of interferon-gamma production; IMP:BHF-UCL.
DR GO; GO:0032733; P:positive regulation of interleukin-10 production; IMP:BHF-UCL.
DR GO; GO:0032735; P:positive regulation of interleukin-12 production; IMP:BHF-UCL.
DR GO; GO:0032736; P:positive regulation of interleukin-13 production; IDA:BHF-UCL.
DR GO; GO:0032743; P:positive regulation of interleukin-2 production; ISO:MGI.
DR GO; GO:0032753; P:positive regulation of interleukin-4 production; IDA:BHF-UCL.
DR GO; GO:2000572; P:positive regulation of interleukin-4-dependent isotype switching to IgE isotypes; IMP:BHF-UCL.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; IMP:BHF-UCL.
DR GO; GO:2000568; P:positive regulation of memory T cell activation; IMP:BHF-UCL.
DR GO; GO:0043382; P:positive regulation of memory T cell differentiation; IDA:BHF-UCL.
DR GO; GO:2000525; P:positive regulation of T cell costimulation; IDA:BHF-UCL.
DR GO; GO:0002726; P:positive regulation of T cell cytokine production; IDA:BHF-UCL.
DR GO; GO:2000406; P:positive regulation of T cell migration; NAS:BHF-UCL.
DR GO; GO:0042102; P:positive regulation of T cell proliferation; IBA:GO_Central.
DR GO; GO:2000570; P:positive regulation of T-helper 2 cell activation; IMP:BHF-UCL.
DR GO; GO:0045630; P:positive regulation of T-helper 2 cell differentiation; IDA:BHF-UCL.
DR GO; GO:0002830; P:positive regulation of type 2 immune response; IDA:BHF-UCL.
DR GO; GO:0002819; P:regulation of adaptive immune response; IDA:BHF-UCL.
DR GO; GO:0050727; P:regulation of inflammatory response; IDA:BHF-UCL.
DR GO; GO:0035713; P:response to nitrogen dioxide; IDA:BHF-UCL.
DR GO; GO:0009615; P:response to virus; IDA:BHF-UCL.
DR GO; GO:0042098; P:T cell proliferation; ISO:MGI.
DR GO; GO:0035712; P:T-helper 2 cell activation; IMP:BHF-UCL.
DR CDD; cd00184; TNF; 1.
DR Gene3D; 2.60.120.40; -; 1.
DR InterPro; IPR021184; TNF_CS.
DR InterPro; IPR006052; TNF_dom.
DR InterPro; IPR042338; TNFSF4.
DR InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR PANTHER; PTHR17534; PTHR17534; 1.
DR SMART; SM00207; TNF; 1.
DR SUPFAM; SSF49842; SSF49842; 1.
DR PROSITE; PS00251; TNF_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytokine; Disulfide bond; Glycoprotein; Membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..198
FT /note="Tumor necrosis factor ligand superfamily member 4"
FT /id="PRO_0000185494"
FT TOPO_DOM 1..28
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..50
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:16905106"
FT DISULFID 70..163
FT /evidence="ECO:0000269|PubMed:16905106"
FT DISULFID 98..183
FT /evidence="ECO:0000269|PubMed:16905106"
FT STRAND 62..71
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 74..78
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 81..83
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 87..90
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 100..112
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 116..119
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 135..146
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 151..156
FT /evidence="ECO:0007829|PDB:2HEW"
FT HELIX 160..165
FT /evidence="ECO:0007829|PDB:2HEW"
FT STRAND 168..177
FT /evidence="ECO:0007829|PDB:2HEW"
SQ SEQUENCE 198 AA; 22255 MW; F4B23A7CC0590459 CRC64;
MEGEGVQPLD ENLENGSRPR FKWKKTLRLV VSGIKGAGML LCFIYVCLQL SSSPAKDPPI
QRLRGAVTRC EDGQLFISSY KNEYQTMEVQ NNSVVIKCDG LYIIYLKGSF FQEVKIDLHF
REDHNPISIP MLNDGRRIVF TVVASLAFKD KVYLTVNAPD TLCEHLQIND GELIVVQLTP
GYCAPEGSYH STVNQVPL
