TNNC1_BLAGE
ID TNNC1_BLAGE Reviewed; 151 AA.
AC Q1A7B3;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=Troponin C, isoallergen Bla g 6.0101 {ECO:0000303|PubMed:16751002};
DE Short=NACP;
DE AltName: Allergen=Bla g 6.0101 {ECO:0000303|PubMed:16751002};
OS Blattella germanica (German cockroach) (Blatta germanica).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blaberoidea; Ectobiidae;
OC Blattellinae; Blattella.
OX NCBI_TaxID=6973 {ECO:0000312|EMBL:ABB89296.1};
RN [1] {ECO:0000312|EMBL:ABB89296.1}
RP NUCLEOTIDE SEQUENCE [MRNA], 3D-STRUCTURE MODELING, AND ALLERGEN.
RX PubMed=16751002; DOI=10.1016/j.jaci.2006.02.017;
RA Hindley J., Wunschmann S., Satinover S.M., Woodfolk J.A., Chew F.T.,
RA Chapman M.D., Pomes A.;
RT "Bla g 6: a troponin C allergen from Blattella germanica with IgE binding
RT calcium dependence.";
RL J. Allergy Clin. Immunol. 117:1389-1395(2006).
CC -!- FUNCTION: Troponin is the central regulatory protein of striated muscle
CC contraction. It consists of three components: Troponin-I (Tn-I) which
CC is the inhibitor of actomyosin ATPase, Troponin-T (Tn-T) which contains
CC the binding site for tropomyosin and Troponin-C (Tn-C). The binding of
CC calcium to Tn-C abolishes the inhibitory action of Tn on actin
CC filaments. {ECO:0000305}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to cockroach. Binds to IgE in 86% of the 7 sera
CC tested that also bind Bla g 6.0201 isoallergen. Calcium depletion by 10
CC mM ethylene glycol bis(2-aminoethyl)tetraacetic acid (EGTA) does not
CC significantly affect IgE-binding, but addition of 10 mM CaCl(2) after
CC calcium depletion increases IgE-binding by approximately 2-fold.
CC {ECO:0000269|PubMed:16751002}.
CC -!- SIMILARITY: Belongs to the troponin C family. {ECO:0000305}.
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DR EMBL; DQ279092; ABB89296.1; -; mRNA.
DR AlphaFoldDB; Q1A7B3; -.
DR SMR; Q1A7B3; -.
DR Allergome; 145; Bla g 6.
DR Allergome; 2865; Bla g 6.0101.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW Allergen; Calcium; Metal-binding; Muscle protein; Repeat.
FT CHAIN 1..151
FT /note="Troponin C, isoallergen Bla g 6.0101"
FT /id="PRO_0000447466"
FT DOMAIN 7..42
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 43..78
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 83..118
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 119..151
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 56
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 58
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 60
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 62
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 67
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 132
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 134
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 136
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 138
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 143
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 151 AA; 17216 MW; BDC87463A72935AF CRC64;
MDELPPEQIQ LLKKAFDAFD REKKGCISTE MVGTILEMLG HRLDDDMLQE IIAEVDADGS
GELEFEEFVS LASRFLVEED AEAMQQELRE AFRLYDKEGN GYITTNVLRE ILKELDDKIT
AEDLDMMIEE IDSDGSGTVD FDEFMEVMTG E
