BTF3_CAEEL
ID BTF3_CAEEL Reviewed; 161 AA.
AC Q18885;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Transcription factor BTF3 homolog {ECO:0000305};
DE AltName: Full=Inhibitor of cell death 1 {ECO:0000312|WormBase:C56C10.8};
DE AltName: Full=Nascent polypeptide-associated complex subunit beta {ECO:0000305};
DE Short=NAC-beta {ECO:0000305};
GN Name=icd-1 {ECO:0000312|WormBase:C56C10.8};
GN ORFNames=C56C10.8 {ECO:0000312|WormBase:C56C10.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12944970; DOI=10.1038/nature01920;
RA Bloss T.A., Witze E.S., Rothman J.H.;
RT "Suppression of CED-3-independent apoptosis by mitochondrial betaNAC in
RT Caenorhabditis elegans.";
RL Nature 424:1066-1071(2003).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22957041; DOI=10.1371/journal.pone.0044038;
RA Arsenovic P.T., Maldonado A.T., Colleluori V.D., Bloss T.A.;
RT "Depletion of the C. elegans NAC engages the unfolded protein response,
RT resulting in increased chaperone expression and apoptosis.";
RL PLoS ONE 7:e44038-e44038(2012).
CC -!- FUNCTION: May be a general transcription factor that can form a stable
CC complex with RNA polymerase II (By similarity). May prevent
CC inappropriate targeting of non-secretory polypeptides to the
CC endoplasmic reticulum (ER) (By similarity). In response to heat stress,
CC plays a role in modulating the unfolded protein response by negatively
CC regulating the accumulation of proteins such as the chaperone protein
CC hsp-4 in the endoplasmic reticulum (PubMed:22957041). Required to
CC suppress apoptosis in neuronal and intestinal cells to promote
CC development (PubMed:12944970). {ECO:0000250|UniProtKB:P20290,
CC ECO:0000269|PubMed:12944970, ECO:0000269|PubMed:22957041}.
CC -!- SUBUNIT: May be part of the nascent polypeptide-associated complex
CC (NAC), which is a heterodimer of icd-2 and icd-1 (via NAC-A/B domains).
CC {ECO:0000250|UniProtKB:P20290}.
CC -!- INTERACTION:
CC Q18885; Q86S66: icd-2; NbExp=6; IntAct=EBI-326530, EBI-326549;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Cytoplasm
CC {ECO:0000250|UniProtKB:P20290}. Mitochondrion
CC {ECO:0000269|PubMed:12944970}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:12944970}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown mostly results in
CC embryonic lethality, but embryos that do hatch produce larvae with a
CC large number of apoptotic cell corpses, which are first present at the
CC comma stage of development (PubMed:12944970). Surviving animals are
CC uncoordinated (PubMed:12944970). RNAi-mediated knockdown results in
CC increased neuronal and intestinal cell death and a reduced number of
CC male tail rays in larvae (PubMed:12944970). RNAi-mediated knockdown in
CC larvae results in increased survival and mobility in response to a
CC constant temperature of 36 degrees Celsius, when compared to wild-type
CC (PubMed:22957041). RNAi-mediated knockdown in adults results in embryos
CC with severe morphological defects and high numbers of enlarged bi-
CC refringent lipofuscin glo-1-positive granules in their gut cells, and
CC increased cell death and degeneration (PubMed:22957041). These embryos
CC also have increased expression of the endoplasmic reticulum-specific
CC chaperone protein hsp-4 in regions of the embryo that contain gut and
CC epithelial cells (PubMed:22957041). {ECO:0000269|PubMed:12944970,
CC ECO:0000269|PubMed:22957041}.
CC -!- SIMILARITY: Belongs to the NAC-beta family. {ECO:0000305}.
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DR EMBL; BX284602; CCD68179.1; -; Genomic_DNA.
DR PIR; T15847; T15847.
DR RefSeq; NP_495336.1; NM_062935.6.
DR AlphaFoldDB; Q18885; -.
DR SMR; Q18885; -.
DR BioGRID; 39428; 117.
DR ComplexPortal; CPX-5744; Nascent polypeptide-associated complex.
DR DIP; DIP-27302N; -.
DR IntAct; Q18885; 3.
DR STRING; 6239.C56C10.8.1; -.
DR iPTMnet; Q18885; -.
DR EPD; Q18885; -.
DR PaxDb; Q18885; -.
DR PeptideAtlas; Q18885; -.
DR EnsemblMetazoa; C56C10.8.1; C56C10.8.1; WBGene00002045.
DR GeneID; 174090; -.
DR KEGG; cel:CELE_C56C10.8; -.
DR UCSC; C56C10.8.1; c. elegans.
DR CTD; 174090; -.
DR WormBase; C56C10.8; CE02573; WBGene00002045; icd-1.
DR eggNOG; KOG2240; Eukaryota.
DR GeneTree; ENSGT00940000153288; -.
DR HOGENOM; CLU_098726_3_0_1; -.
DR InParanoid; Q18885; -.
DR OMA; KVGDCKH; -.
DR OrthoDB; 1435264at2759; -.
DR PhylomeDB; Q18885; -.
DR SignaLink; Q18885; -.
DR PRO; PR:Q18885; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00002045; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:WormBase.
DR GO; GO:0005854; C:nascent polypeptide-associated complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0042788; C:polysomal ribosome; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:WormBase.
DR GO; GO:1905551; P:negative regulation of protein localization to endoplasmic reticulum; IC:ComplexPortal.
DR GO; GO:1900034; P:regulation of cellular response to heat; IC:ComplexPortal.
DR Gene3D; 2.20.70.30; -; 1.
DR InterPro; IPR039370; BTF3.
DR InterPro; IPR038187; NAC_A/B_dom_sf.
DR InterPro; IPR002715; Nas_poly-pep-assoc_cplx_dom.
DR PANTHER; PTHR10351; PTHR10351; 1.
DR Pfam; PF01849; NAC; 1.
DR SMART; SM01407; NAC; 1.
DR PROSITE; PS51151; NAC_AB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Mitochondrion; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..161
FT /note="Transcription factor BTF3 homolog"
FT /id="PRO_0000213550"
FT DOMAIN 40..105
FT /note="NAC-A/B"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00507"
FT REGION 16..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 128..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 161 AA; 17503 MW; 12E24FF70D12BE94 CRC64;
MDSKAIAERI KKLQAQQEHV RIGGKGTPRR KKKVIHKTAA ADDKKLQSNL KKLSVTNIPG
IEEVNMIKDD GTVIHFNNPK VQTSVPANTF SVTGSADNKQ ITEMLPGILN QLGPESLTHL
KKLANNVTKL GPDGKGEDED VPELVGDFDA ASKNETKADE Q
