BTG2_MOUSE
ID BTG2_MOUSE Reviewed; 158 AA.
AC Q04211;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Protein BTG2;
DE AltName: Full=BTG family member 2;
DE AltName: Full=NGF-inducible protein TIS21;
GN Name=Btg2; Synonyms=Tis21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX PubMed=1713584; DOI=10.1016/s0021-9258(18)98716-8;
RA Fletcher B.S., Lim R.W., Varnum B.C., Kujubu D.A., Koski R.A.,
RA Herschman H.R.;
RT "Structure and expression of TIS21, a primary response gene induced by
RT growth factors and tumor promoters.";
RL J. Biol. Chem. 266:14511-14518(1991).
CC -!- FUNCTION: Anti-proliferative protein; the function is mediated by
CC association with deadenylase subunits of the CCR4-NOT complex.
CC Activates mRNA deadenylation in a CNOT6 and CNOT7-dependent manner. In
CC vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in
CC cell cycle regulation. Could be involved in the growth arrest and
CC differentiation of the neuronal precursors. Modulates transcription
CC regulation mediated by ESR1. Involved in mitochondrial depolarization
CC and neurite outgrowth (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with PRKCABP. Interacts with CNOT7 and CNOT8;
CC indicative for an association with the CCR4-NOT complex. Interacts with
CC PIN1, inducing mitochondrial depolarization (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q04211; Q60809: Cnot7; NbExp=2; IntAct=EBI-7847081, EBI-2104739;
CC Q04211; Q9D8X5: Cnot8; NbExp=3; IntAct=EBI-7847081, EBI-16204625;
CC -!- INDUCTION: By nerve growth factor and tumors promoters.
CC {ECO:0000269|PubMed:1713584}.
CC -!- PTM: Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at
CC Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial
CC depolarization. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BTG family. {ECO:0000305}.
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DR EMBL; M64292; AAA40449.1; -; Genomic_DNA.
DR CCDS; CCDS15302.1; -.
DR PIR; A40761; A40761.
DR RefSeq; NP_031596.1; NM_007570.2.
DR PDB; 3DJN; X-ray; 2.20 A; B=8-125.
DR PDBsum; 3DJN; -.
DR AlphaFoldDB; Q04211; -.
DR SMR; Q04211; -.
DR BioGRID; 198398; 3.
DR DIP; DIP-41601N; -.
DR IntAct; Q04211; 3.
DR MINT; Q04211; -.
DR STRING; 10090.ENSMUSP00000020692; -.
DR iPTMnet; Q04211; -.
DR PhosphoSitePlus; Q04211; -.
DR PaxDb; Q04211; -.
DR PRIDE; Q04211; -.
DR ProteomicsDB; 265386; -.
DR Antibodypedia; 1189; 330 antibodies from 29 providers.
DR DNASU; 12227; -.
DR Ensembl; ENSMUST00000020692; ENSMUSP00000020692; ENSMUSG00000020423.
DR GeneID; 12227; -.
DR KEGG; mmu:12227; -.
DR UCSC; uc007cre.1; mouse.
DR CTD; 7832; -.
DR MGI; MGI:108384; Btg2.
DR VEuPathDB; HostDB:ENSMUSG00000020423; -.
DR eggNOG; KOG4006; Eukaryota.
DR GeneTree; ENSGT00950000182952; -.
DR HOGENOM; CLU_079660_4_0_1; -.
DR InParanoid; Q04211; -.
DR OMA; SYGMLTC; -.
DR OrthoDB; 1439942at2759; -.
DR PhylomeDB; Q04211; -.
DR TreeFam; TF105272; -.
DR Reactome; R-MMU-6804115; TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
DR BioGRID-ORCS; 12227; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Btg2; mouse.
DR EvolutionaryTrace; Q04211; -.
DR PRO; PR:Q04211; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q04211; protein.
DR Bgee; ENSMUSG00000020423; Expressed in granulocyte and 260 other tissues.
DR ExpressionAtlas; Q04211; baseline and differential.
DR Genevisible; Q04211; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; ISO:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0008306; P:associative learning; IMP:MGI.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:MGI.
DR GO; GO:0021954; P:central nervous system neuron development; IMP:MGI.
DR GO; GO:0021542; P:dentate gyrus development; IMP:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0045930; P:negative regulation of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:2000178; P:negative regulation of neural precursor cell proliferation; IMP:MGI.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0017148; P:negative regulation of translation; ISS:UniProtKB.
DR GO; GO:0061351; P:neural precursor cell proliferation; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; ISO:MGI.
DR GO; GO:0031175; P:neuron projection development; ISO:MGI.
DR GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISO:MGI.
DR GO; GO:0006479; P:protein methylation; IDA:MGI.
DR GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl.
DR GO; GO:0009612; P:response to mechanical stimulus; IEA:Ensembl.
DR GO; GO:0014070; P:response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0043434; P:response to peptide hormone; IEA:Ensembl.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
DR Gene3D; 3.90.640.90; -; 1.
DR InterPro; IPR002087; Anti_prolifrtn.
DR InterPro; IPR033332; BTG.
DR InterPro; IPR036054; BTG-like_sf.
DR InterPro; IPR033328; BTG2.
DR PANTHER; PTHR22978; PTHR22978; 1.
DR PANTHER; PTHR22978:SF29; PTHR22978:SF29; 1.
DR Pfam; PF07742; BTG; 1.
DR PRINTS; PR00310; ANTIPRLFBTG1.
DR SMART; SM00099; btg1; 1.
DR SUPFAM; SSF160696; SSF160696; 1.
DR PROSITE; PS00960; BTG_1; 1.
DR PROSITE; PS01203; BTG_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..158
FT /note="Protein BTG2"
FT /id="PRO_0000143805"
FT MOD_RES 147
FT /note="Phosphoserine; by MAPK1 and MAPK3"
FT /evidence="ECO:0000250|UniProtKB:P78543"
FT MOD_RES 149
FT /note="Phosphoserine; by MAPK14"
FT /evidence="ECO:0000250|UniProtKB:P78543"
FT HELIX 10..28
FT /evidence="ECO:0007829|PDB:3DJN"
FT HELIX 32..50
FT /evidence="ECO:0007829|PDB:3DJN"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:3DJN"
FT HELIX 63..66
FT /evidence="ECO:0007829|PDB:3DJN"
FT STRAND 68..73
FT /evidence="ECO:0007829|PDB:3DJN"
FT HELIX 76..84
FT /evidence="ECO:0007829|PDB:3DJN"
FT HELIX 89..95
FT /evidence="ECO:0007829|PDB:3DJN"
FT STRAND 98..105
FT /evidence="ECO:0007829|PDB:3DJN"
FT STRAND 108..114
FT /evidence="ECO:0007829|PDB:3DJN"
FT STRAND 120..124
FT /evidence="ECO:0007829|PDB:3DJN"
SQ SEQUENCE 158 AA; 17682 MW; 5B91563BAE4CB74C CRC64;
MSHGKRTDML PEIAAAVGFL SSLLRTRGCV SEQRLKVFSR ALQDALTDHY KHHWFPEKPS
KGSGYRCIRI NHKMDPIISK VASQIGLSQP QLHRLLPSEL TLWVDPYEVS YRIGEDGSIC
VLYEEAPVAA SYGLLTCKNQ MMLGRSSPSK NYVMAVSS
