BTG2_RAT
ID BTG2_RAT Reviewed; 158 AA.
AC P27049;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein BTG2;
DE AltName: Full=BTG family member 2;
DE AltName: Full=NGF-inducible anti-proliferative protein PC3;
GN Name=Btg2; Synonyms=Pc3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1849653; DOI=10.1073/pnas.88.8.3353;
RA Bradbury A., Possenti R., Shooter E.M., Tirone F.;
RT "Molecular cloning of PC3, a putatively secreted protein whose mRNA is
RT induced by nerve growth factor and depolarization.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:3353-3357(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP CHARACTERIZATION.
RX PubMed=7811636; DOI=10.1016/0925-4773(94)90085-x;
RA Iacopetti P., Barsacchi G., Tirone F., Maffei L., Cremisi F.;
RT "Developmental expression of PC3 gene is correlated with neuronal cell
RT birthday.";
RL Mech. Dev. 47:127-137(1994).
RN [4]
RP CHARACTERIZATION.
RX PubMed=8891336;
RA Montagnoli A., Guardavaccaro D., Starace G., Tirone F.;
RT "Overexpression of the nerve growth factor-inducible PC3 immediate early
RT gene is associated with growth inhibition.";
RL Cell Growth Differ. 7:1327-1336(1996).
RN [5]
RP INTERACTION WITH PRKCABP.
RX PubMed=11237868; DOI=10.1042/0264-6021:3540635;
RA Lin W.-J., Chang Y.-F., Wang W.-L., Huang C.-Y.F.;
RT "Mitogen-stimulated TIS21 protein interacts with a protein-kinase-Calpha-
RT binding protein rPICK1.";
RL Biochem. J. 354:635-643(2001).
CC -!- FUNCTION: Anti-proliferative protein; the function is mediated by
CC association with deadenylase subunits of the CCR4-NOT complex.
CC Activates mRNA deadenylation in a CNOT6 and CNOT7-dependent manner. In
CC vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in
CC cell cycle regulation. Could be involved in the growth arrest and
CC differentiation of the neuronal precursors. Modulates transcription
CC regulation mediated by ESR1. Involved in mitochondrial depolarization
CC and neurite outgrowth (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with PRKCABP. Interacts with CNOT7 and CNOT8;
CC indicative for an association with the CCR4-NOT complex. Interacts with
CC PIN1, inducing mitochondrial depolarization.
CC {ECO:0000269|PubMed:11237868}.
CC -!- INTERACTION:
CC P27049; Q9EP80: Pick1; NbExp=3; IntAct=EBI-78953, EBI-77728;
CC -!- TISSUE SPECIFICITY: In brain at embryonic day 13.5, placenta, amnion,
CC and spleen, which are proliferating and/or differentiating.
CC -!- INDUCTION: By nerve growth factor. At lower levels by epidermal growth
CC factor and membrane depolarization. At much lower levels by fibroblast
CC growth factor and interleukin 6.
CC -!- PTM: Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at
CC Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial
CC depolarization. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BTG family. {ECO:0000305}.
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DR EMBL; M60921; AAB49567.1; -; mRNA.
DR EMBL; BC072493; AAH72493.1; -; mRNA.
DR PIR; A40443; A40443.
DR RefSeq; NP_058955.1; NM_017259.1.
DR AlphaFoldDB; P27049; -.
DR SMR; P27049; -.
DR BioGRID; 248245; 3.
DR IntAct; P27049; 2.
DR STRING; 10116.ENSRNOP00000004408; -.
DR PaxDb; P27049; -.
DR Ensembl; ENSRNOT00000004408; ENSRNOP00000004408; ENSRNOG00000003300.
DR GeneID; 29619; -.
DR KEGG; rno:29619; -.
DR UCSC; RGD:2225; rat.
DR CTD; 7832; -.
DR RGD; 2225; Btg2.
DR eggNOG; KOG4006; Eukaryota.
DR GeneTree; ENSGT00950000182952; -.
DR HOGENOM; CLU_079660_4_0_1; -.
DR InParanoid; P27049; -.
DR OMA; SYGMLTC; -.
DR OrthoDB; 1439942at2759; -.
DR PhylomeDB; P27049; -.
DR TreeFam; TF105272; -.
DR Reactome; R-RNO-6804115; TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
DR PRO; PR:P27049; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000003300; Expressed in thymus and 20 other tissues.
DR Genevisible; P27049; RN.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; ISO:RGD.
DR GO; GO:0008306; P:associative learning; ISO:RGD.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:RGD.
DR GO; GO:0021954; P:central nervous system neuron development; ISO:RGD.
DR GO; GO:0021542; P:dentate gyrus development; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0045930; P:negative regulation of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:2000178; P:negative regulation of neural precursor cell proliferation; ISO:RGD.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IMP:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0017148; P:negative regulation of translation; ISS:UniProtKB.
DR GO; GO:0061351; P:neural precursor cell proliferation; IEA:Ensembl.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0031175; P:neuron projection development; ISO:RGD.
DR GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISO:RGD.
DR GO; GO:0006479; P:protein methylation; ISO:RGD.
DR GO; GO:0051602; P:response to electrical stimulus; IEP:RGD.
DR GO; GO:0009612; P:response to mechanical stimulus; IEP:RGD.
DR GO; GO:0014070; P:response to organic cyclic compound; IEP:RGD.
DR GO; GO:0010033; P:response to organic substance; IEP:RGD.
DR GO; GO:0010243; P:response to organonitrogen compound; IEP:RGD.
DR GO; GO:0043434; P:response to peptide hormone; IEP:RGD.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; ISO:RGD.
DR Gene3D; 3.90.640.90; -; 1.
DR InterPro; IPR002087; Anti_prolifrtn.
DR InterPro; IPR033332; BTG.
DR InterPro; IPR036054; BTG-like_sf.
DR InterPro; IPR033328; BTG2.
DR PANTHER; PTHR22978; PTHR22978; 1.
DR PANTHER; PTHR22978:SF29; PTHR22978:SF29; 1.
DR Pfam; PF07742; BTG; 1.
DR PRINTS; PR00310; ANTIPRLFBTG1.
DR SMART; SM00099; btg1; 1.
DR SUPFAM; SSF160696; SSF160696; 1.
DR PROSITE; PS00960; BTG_1; 1.
DR PROSITE; PS01203; BTG_2; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..158
FT /note="Protein BTG2"
FT /id="PRO_0000143806"
FT MOD_RES 147
FT /note="Phosphoserine; by MAPK1 and MAPK3"
FT /evidence="ECO:0000250|UniProtKB:P78543"
FT MOD_RES 149
FT /note="Phosphoserine; by MAPK14"
FT /evidence="ECO:0000250|UniProtKB:P78543"
SQ SEQUENCE 158 AA; 17728 MW; F9105D73C3CDF24A CRC64;
MSHGKRTDML PEIAAAVGFL TSLLRTRGCV SEQRLKVFSR ALQDALTDHY KHHWFPEKPS
KGSGYRCIRI NHKMDPIISK VASQIGLSQP QLHQLLPSEL TLWVDPYEVS YRIGEDGSIC
VLYEEAPVAT SYGLLTCKNQ MMLGRSSPSK NYVMTVSS
