TNR1_ECOLI
ID TNR1_ECOLI Reviewed; 183 AA.
AC P03012;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=Transposon gamma-delta resolvase;
DE AltName: Full=Transposon Tn1000 resolvase;
GN Name=tnpR; OrderedLocusNames=ECOK12F009;
OS Escherichia coli (strain K12).
OG Plasmid F.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6292730; DOI=10.1038/300381a0;
RA Reed R.R., Shibuya G.I., Steitz J.A.;
RT "Nucleotide sequence of gamma delta resolvase gene and demonstration that
RT its gene product acts as a repressor of transcription.";
RL Nature 300:381-383(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8080658; DOI=10.1266/jjg.69.269;
RA Maekawa T., Ohtsubo E.;
RT "Identification of the region that determines the specificity of binding of
RT the transposases encoded by Tn3 and gamma delta to the terminal inverted
RT repeat sequences.";
RL Jpn. J. Genet. 69:269-285(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7612932; DOI=10.3109/10425179509029361;
RA Broom J.E., Hill D.F., Hughes G., Jones W.A., McNaughton J.C.,
RA Stockwell P.A., Petersen G.B.;
RT "Sequence of a transposon identified as Tn1000 (gamma delta).";
RL DNA Seq. 5:185-189(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / CR63; PLASMID=F;
RA Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT "Complete nucleotide sequence of the F plasmid: its implications for
RT organization and diversification of plasmid genomes.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP ACTIVE SITE SER-10.
RX PubMed=6099239; DOI=10.1101/sqb.1984.049.01.028;
RA Reed R.R., Moser C.D.;
RT "Resolvase-mediated recombination intermediates contain a serine residue
RT covalently linked to DNA.";
RL Cold Spring Harb. Symp. Quant. Biol. 49:245-249(1984).
RN [6]
RP MUTAGENESIS.
RX PubMed=6088082; DOI=10.1016/0092-8674(84)90501-4;
RA Newman B.J., Grindley N.D.F.;
RT "Mutants of the gamma delta resolvase: a genetic analysis of the
RT recombination function.";
RL Cell 38:463-469(1984).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-140.
RX PubMed=2175678; DOI=10.1016/0092-8674(90)90427-g;
RA Sanderson M.R., Freemont P.S., Rice P.A., Goldman A., Hatfull G.F.,
RA Grindley N.D.F., Steitz T.A.;
RT "The crystal structure of the catalytic domain of the site-specific
RT recombination enzyme gamma delta resolvase at 2.7-A resolution.";
RL Cell 63:1323-1329(1990).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX PubMed=7628011; DOI=10.1016/0092-8674(95)90307-0;
RA Yang W., Steitz T.A.;
RT "Crystal structure of the site-specific recombinase gamma delta resolvase
RT complexed with a 34 bp cleavage site.";
RL Cell 82:193-207(1995).
RN [9]
RP STRUCTURE BY NMR OF 141-183.
RX PubMed=7987224; DOI=10.1002/pro.5560030815;
RA Liu T., Derose E.F., Mullen G.P.;
RT "Determination of the structure of the DNA binding domain of gamma delta
RT resolvase in solution.";
RL Protein Sci. 3:1286-1295(1994).
CC -!- FUNCTION: This protein catalyzes the site-specific recombination of the
CC transposon and also regulates its frequency of transposition.
CC -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC {ECO:0000305}.
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DR EMBL; J01844; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; X60200; CAA42759.1; -; Genomic_DNA.
DR EMBL; D16449; BAA03915.1; -; Genomic_DNA.
DR EMBL; AP001918; BAA97879.1; -; Genomic_DNA.
DR PIR; A03542; RPECTG.
DR RefSeq; NP_061388.1; NC_002483.1.
DR RefSeq; WP_001235704.1; NZ_CP047127.1.
DR PDB; 1GDR; X-ray; 3.50 A; A=1-140.
DR PDB; 1GDT; X-ray; 3.00 A; A/B=1-183.
DR PDB; 1GHT; NMR; -; A=1-105.
DR PDB; 1HX7; NMR; -; A=1-105.
DR PDB; 1RES; NMR; -; A=141-183.
DR PDB; 1RET; NMR; -; A=141-183.
DR PDB; 1ZR2; X-ray; 3.90 A; A/B=1-183.
DR PDB; 1ZR4; X-ray; 3.40 A; A/B/D/E=1-183.
DR PDB; 2GM4; X-ray; 3.50 A; A/B=1-183.
DR PDB; 2GM5; X-ray; 2.10 A; A/B/C/D=2-134.
DR PDB; 2RSL; X-ray; 2.30 A; A/B/C=1-140.
DR PDBsum; 1GDR; -.
DR PDBsum; 1GDT; -.
DR PDBsum; 1GHT; -.
DR PDBsum; 1HX7; -.
DR PDBsum; 1RES; -.
DR PDBsum; 1RET; -.
DR PDBsum; 1ZR2; -.
DR PDBsum; 1ZR4; -.
DR PDBsum; 2GM4; -.
DR PDBsum; 2GM5; -.
DR PDBsum; 2RSL; -.
DR AlphaFoldDB; P03012; -.
DR BMRB; P03012; -.
DR SMR; P03012; -.
DR DIP; DIP-61217N; -.
DR PRIDE; P03012; -.
DR PATRIC; fig|83333.107.peg.594; -.
DR PhylomeDB; P03012; -.
DR EvolutionaryTrace; P03012; -.
DR PRO; PR:P03012; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1390; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006118; Recombinase_CS.
DR InterPro; IPR006119; Resolv_N.
DR InterPro; IPR036162; Resolvase-like_N_sf.
DR InterPro; IPR006120; Resolvase_HTH_dom.
DR Pfam; PF02796; HTH_7; 1.
DR Pfam; PF00239; Resolvase; 1.
DR SMART; SM00857; Resolvase; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF53041; SSF53041; 1.
DR PROSITE; PS00397; RECOMBINASES_1; 1.
DR PROSITE; PS00398; RECOMBINASES_2; 1.
DR PROSITE; PS51736; RECOMBINASES_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA integration; DNA recombination; DNA-binding; Plasmid;
KW Transposable element.
FT CHAIN 1..183
FT /note="Transposon gamma-delta resolvase"
FT /id="PRO_0000196367"
FT DOMAIN 2..137
FT /note="Resolvase/invertase-type recombinase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT DNA_BIND 161..180
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT ACT_SITE 10
FT /note="O-(5'-phospho-DNA)-serine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072,
FT ECO:0000269|PubMed:6099239"
FT STRAND 3..11
FT /evidence="ECO:0007829|PDB:2GM5"
FT HELIX 13..25
FT /evidence="ECO:0007829|PDB:2GM5"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:2GM5"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:2GM5"
FT STRAND 41..43
FT /evidence="ECO:0007829|PDB:1GDT"
FT HELIX 46..54
FT /evidence="ECO:0007829|PDB:2GM5"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:2GM5"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:2GM5"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:2GM5"
FT HELIX 73..85
FT /evidence="ECO:0007829|PDB:2GM5"
FT STRAND 89..92
FT /evidence="ECO:0007829|PDB:2GM5"
FT TURN 93..96
FT /evidence="ECO:0007829|PDB:2GM5"
FT HELIX 102..127
FT /evidence="ECO:0007829|PDB:2GM5"
FT TURN 135..137
FT /evidence="ECO:0007829|PDB:1ZR4"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:1RES"
FT HELIX 148..156
FT /evidence="ECO:0007829|PDB:1GDT"
FT HELIX 161..168
FT /evidence="ECO:0007829|PDB:1GDT"
FT HELIX 172..180
FT /evidence="ECO:0007829|PDB:1GDT"
SQ SEQUENCE 183 AA; 20362 MW; B2EBA6037F926FAB CRC64;
MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL LRMKVEEGDV
ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD GEMGKMVVTI LSAVAQAERQ
RILERTNEGR QEAMAKGVVF GRKRKIDRDA VLNMWQQGLG ASHISKTMNI ARSTVYKVIN
ESN
