TNR2_ECOLX
ID TNR2_ECOLX Reviewed; 186 AA.
AC P04130;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1986, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Transposon Tn21 resolvase;
GN Name=tnpR;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2992807; DOI=10.1016/0092-8674(85)90120-5;
RA Hyde D.R., Tu C.-P.T.;
RT "tnpM: a novel regulatory gene that enhances Tn21 transposition and
RT suppresses cointegrate resolution.";
RL Cell 42:629-638(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6312271; DOI=10.1007/bf00334812;
RA Diver W.P., Grinsted J., Fritzinger D.C., Brown N.L., Altenbuchner J.,
RA Rogowsky P., Schmitt R.;
RT "DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and
RT Tn1721.";
RL Mol. Gen. Genet. 191:189-193(1983).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 170-186.
RX PubMed=3029727; DOI=10.1093/nar/15.4.1799;
RA Ward E., Grinsted J.;
RT "The nucleotide sequence of the tnpA gene of Tn21.";
RL Nucleic Acids Res. 15:1799-1806(1987).
CC -!- FUNCTION: Resolvase catalyzes the resolution (a site-specific
CC recombination) of the cointegrated replicon to yield the final
CC transposition products.
CC -!- MISCELLANEOUS: Tn21 is a transposon encoding resistance to sulfonamide,
CC streptomycin, and mercuric ion.
CC -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC {ECO:0000305}.
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DR EMBL; X01298; CAA25626.1; -; Genomic_DNA.
DR EMBL; M10791; AAA27421.1; -; Genomic_DNA.
DR EMBL; X04891; CAA28578.1; -; Genomic_DNA.
DR PIR; A03543; RPEC21.
DR RefSeq; WP_000147567.1; NZ_WVVM01000039.1.
DR RefSeq; YP_001096360.1; NC_009132.1.
DR RefSeq; YP_001096416.1; NC_009133.1.
DR RefSeq; YP_001816594.1; NC_010558.1.
DR RefSeq; YP_006903512.1; NC_019043.1.
DR RefSeq; YP_006953996.1; NC_019091.1.
DR RefSeq; YP_008864012.1; NC_022992.1.
DR RefSeq; YP_008864679.1; NC_022996.1.
DR RefSeq; YP_008995269.1; NC_023277.2.
DR RefSeq; YP_008997427.1; NC_023289.2.
DR RefSeq; YP_009060119.1; NC_024956.1.
DR RefSeq; YP_009061091.1; NC_024975.1.
DR RefSeq; YP_009071096.1; NC_025179.1.
DR AlphaFoldDB; P04130; -.
DR SMR; P04130; -.
DR OMA; HLMADST; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1390; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006118; Recombinase_CS.
DR InterPro; IPR006119; Resolv_N.
DR InterPro; IPR036162; Resolvase-like_N_sf.
DR InterPro; IPR006120; Resolvase_HTH_dom.
DR Pfam; PF02796; HTH_7; 1.
DR Pfam; PF00239; Resolvase; 1.
DR SMART; SM00857; Resolvase; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF53041; SSF53041; 1.
DR PROSITE; PS00397; RECOMBINASES_1; 1.
DR PROSITE; PS00398; RECOMBINASES_2; 1.
DR PROSITE; PS51736; RECOMBINASES_3; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; DNA-binding; Transposable element.
FT CHAIN 1..186
FT /note="Transposon Tn21 resolvase"
FT /id="PRO_0000196372"
FT DOMAIN 4..137
FT /note="Resolvase/invertase-type recombinase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT DNA_BIND 164..183
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT ACT_SITE 12
FT /note="O-(5'-phospho-DNA)-serine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ SEQUENCE 186 AA; 21329 MW; 93928B164D3D08CF CRC64;
MTGQRIGYIR VSTFDQNPER QLEGVKVDRA FSDKASGKDV KRPQLEALIS FARTGDTVVV
HSMDRLARNL DDLRRIVQTL TQRGVHIEFV KEHLSFTGED SPMANLMLSV MGAFAEFERA
LIRERQREGI ALAKQRGAYR GRKKSLSSER IAELRQRVEA GEQKTKLARE FGISRETLYQ
YLRTDQ
