ID   TNR3_ECOLX              Reviewed;         185 AA.
AC   P0ADI2; P03011;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Transposon Tn3 resolvase;
GN   Name=tnpR;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=391406; DOI=10.1016/0092-8674(79)90228-9;
RA   Heffron F., McCarthy B.J., Ohtsubo H., Ohtsubo E.;
RT   "DNA sequence analysis of the transposon Tn3: three genes and three sites
RT   involved in transposition of Tn3.";
RL   Cell 18:1153-1163(1979).
RN   [2]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
CC   -!- FUNCTION: Resolvase catalyzes the resolution (a site-specific
CC       recombination) of the cointegrated replicon to yield the final
CC       transposition products.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; V00613; CAA23885.1; -; Genomic_DNA.
DR   PIR; A03541; RPECT.
DR   RefSeq; NP_943294.1; NC_005248.1.
DR   RefSeq; WP_001217881.1; NZ_WWEV01000054.1.
DR   RefSeq; YP_001965402.1; NC_010862.1.
DR   RefSeq; YP_006939985.1; NC_018994.1.
DR   RefSeq; YP_006952420.1; NC_019062.1.
DR   RefSeq; YP_006953597.1; NC_019079.1.
DR   RefSeq; YP_009022937.1; NC_023907.1.
DR   RefSeq; YP_009060581.1; NC_024967.1.
DR   RefSeq; YP_190221.1; NC_006671.1.
DR   PDB; 5CY1; X-ray; 3.40 A; A/B=1-185.
DR   PDB; 5CY2; X-ray; 4.00 A; A/B/E/F=1-185.
DR   PDBsum; 5CY1; -.
DR   PDBsum; 5CY2; -.
DR   AlphaFoldDB; P0ADI2; -.
DR   SMR; P0ADI2; -.
DR   PRIDE; P0ADI2; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA integration; DNA recombination; DNA-binding;
KW   Transposable element.
FT   CHAIN           1..185
FT                   /note="Transposon Tn3 resolvase"
FT                   /id="PRO_0000196368"
FT   DOMAIN          2..137
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        161..180
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        10
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   STRAND          3..8
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           14..25
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           30..32
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   STRAND          33..36
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           46..54
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   STRAND          60..64
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           66..68
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   STRAND          70..72
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           73..85
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   STRAND          89..92
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   TURN            93..96
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   STRAND          97..100
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   TURN            101..103
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           104..128
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           148..157
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           161..168
FT                   /evidence="ECO:0007829|PDB:5CY1"
FT   HELIX           172..185
FT                   /evidence="ECO:0007829|PDB:5CY1"
SQ   SEQUENCE   185 AA;  20618 MW;  A160E3DBFAF59C00 CRC64;
     MRIFGYARVS TSQQSLDIQI RALKDAGVKA NRIFTDKASG SSTDREGLDL LRMKVEEGDV
     ILVKKLDRLG RDTADMIQLI KEFDAQGVAV RFIDDGISTD GDMGQMVVTI LSAVAQAERR
     RILERTNEGR QEAKLKGIKF GRRRTVDRNV VLTLHQKGTG ATEIAHQLSI ARSTVYKILE
     DERAS