ID   TNR3_KLEPN              Reviewed;         185 AA.
AC   P0ADI3; P03011;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Transposon Tn3 resolvase;
GN   Name=tnpR;
OS   Klebsiella pneumoniae.
OG   Plasmid pCFF04.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1331747; DOI=10.1007/bf00286188;
RA   Mabilat C., Lourencao-Vital J., Goussard S., Courvalin P.;
RT   "A new example of physical linkage between Tn1 and Tn21: the antibiotic
RT   multiple-resistance region of plasmid pCFF04 encoding extended-spectrum
RT   beta-lactamase TEM-3.";
RL   Mol. Gen. Genet. 235:113-121(1992).
CC   -!- FUNCTION: Resolvase catalyzes the resolution (a site-specific
CC       recombination) of the cointegrated replicon to yield the final
CC       transposition products.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X64523; CAA45829.1; -; Genomic_DNA.
DR   PIR; S30114; S30114.
DR   RefSeq; NP_608306.1; NC_003486.1.
DR   RefSeq; WP_001217881.1; NZ_WWPX01000044.1.
DR   RefSeq; YP_001928082.1; NC_010726.1.
DR   RefSeq; YP_002286820.1; NC_011382.1.
DR   RefSeq; YP_002286970.1; NC_011385.1.
DR   RefSeq; YP_003754014.1; NC_014312.1.
DR   RefSeq; YP_006958959.1; NC_019156.1.
DR   RefSeq; YP_006959189.1; NC_019159.1.
DR   RefSeq; YP_007195422.1; NC_019888.1.
DR   RefSeq; YP_007878587.1; NC_021079.1.
DR   RefSeq; YP_008166966.1; NC_021666.1.
DR   RefSeq; YP_008603394.1; NC_022520.1.
DR   RefSeq; YP_008998914.1; NC_023331.1.
DR   RefSeq; YP_009067784.1; NC_025131.1.
DR   RefSeq; YP_009067869.1; NC_025134.1.
DR   RefSeq; YP_009071731.1; NC_025185.1.
DR   AlphaFoldDB; P0ADI3; -.
DR   SMR; P0ADI3; -.
DR   OMA; PQYTQDD; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Transposable element.
FT   CHAIN           1..185
FT                   /note="Transposon Tn3 resolvase"
FT                   /id="PRO_0000196369"
FT   DOMAIN          2..137
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        161..180
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        10
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   185 AA;  20618 MW;  A160E3DBFAF59C00 CRC64;
     MRIFGYARVS TSQQSLDIQI RALKDAGVKA NRIFTDKASG SSTDREGLDL LRMKVEEGDV
     ILVKKLDRLG RDTADMIQLI KEFDAQGVAV RFIDDGISTD GDMGQMVVTI LSAVAQAERR
     RILERTNEGR QEAKLKGIKF GRRRTVDRNV VLTLHQKGTG ATEIAHQLSI ARSTVYKILE
     DERAS