ID   TNR6_ENTFC              Reviewed;         191 AA.
AC   Q06237;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Transposon Tn1546 resolvase;
OS   Enterococcus faecium (Streptococcus faecium).
OG   Plasmid pIP816.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1352;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BM4147;
RX   PubMed=8380148; DOI=10.1128/jb.175.1.117-127.1993;
RA   Arthur M., Molinas C., Depardieu F., Courvalin P.;
RT   "Characterization of Tn1546, a Tn3-related transposon conferring
RT   glycopeptide resistance by synthesis of depsipeptide peptidoglycan
RT   precursors in Enterococcus faecium BM4147.";
RL   J. Bacteriol. 175:117-127(1993).
CC   -!- FUNCTION: Resolvase catalyzes the resolution (a site-specific
CC       recombination) of the cointegrated replicon to yield the final
CC       transposition products.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; M97297; AAA65952.1; -; Genomic_DNA.
DR   PIR; B40628; B40628.
DR   RefSeq; WP_001226076.1; NZ_WSZC01000102.1.
DR   RefSeq; YP_001019039.1; NC_008821.1.
DR   RefSeq; YP_001974800.1; NC_010980.1.
DR   RefSeq; YP_002128395.1; NC_011140.1.
DR   RefSeq; YP_004172620.1; NC_014959.1.
DR   RefSeq; YP_976081.1; NC_008768.1.
DR   AlphaFoldDB; Q06237; -.
DR   SMR; Q06237; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Transposable element.
FT   CHAIN           1..191
FT                   /note="Transposon Tn1546 resolvase"
FT                   /id="PRO_0000196374"
FT   DOMAIN          2..138
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        168..187
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        10
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   191 AA;  22296 MW;  B027350864EFC17C CRC64;
     MRKIGYIRVS STNQNPSRQF QQLNEIGMDI IYEEKVSGAT KDREQLQKVL DDLQEDDIIY
     VTDLTRITRS TQDLFELIDN IRDKKASLKS LKDTWLDLSE DNPYSQFLIT VMAGVNQLER
     DLIRMRQREG IELAKKEGKF KGRLKKYHKN HAGMNYAVKL YKEGNMTVNQ ICEITNVSRA
     SLYRKLSEVN N