ID   TNR6_ENTFL              Reviewed;         405 AA.
AC   P22886;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Transposase from transposon Tn916;
DE   AltName: Full=Integrase;
GN   Name=Int-Tn;
OS   Enterococcus faecalis (Streptococcus faecalis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DS16; TRANSPOSON=Tn916;
RA   Clewell D.B., Flannagan S.E., Zitzow L.A., Su Y.A., He P., Senghas E.,
RA   Weaver K.E.;
RL   Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DS16; TRANSPOSON=Tn916;
RX   PubMed=7899523; DOI=10.1006/plas.1994.1077;
RA   Flannagan S.E., Zitzow L.A., Su Y.A., Clewell D.B.;
RT   "Nucleotide sequence of the 18-kb conjugative transposon Tn916 from
RT   Enterococcus faecalis.";
RL   Plasmid 32:350-354(1994).
RN   [3]
RP   STRUCTURE BY NMR OF 3-73.
RX   PubMed=9665166; DOI=10.1038/799;
RA   Connolly K.M., Wojciak J.M., Clubb R.T.;
RT   "Site-specific DNA binding using a variation of the double stranded RNA
RT   binding motif.";
RL   Nat. Struct. Biol. 5:546-550(1998).
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M37184; AAA19427.1; -; Genomic_DNA.
DR   EMBL; U09422; AAB60030.1; -; Genomic_DNA.
DR   RefSeq; WP_010785527.1; NZ_NSJS01000002.1.
DR   PDB; 1B69; NMR; -; A=3-71.
DR   PDB; 1BB8; NMR; -; A=3-73.
DR   PDB; 1TN9; NMR; -; A=3-71.
DR   PDB; 2BB8; NMR; -; A=3-73.
DR   PDBsum; 1B69; -.
DR   PDBsum; 1BB8; -.
DR   PDBsum; 1TN9; -.
DR   PDBsum; 2BB8; -.
DR   AlphaFoldDB; P22886; -.
DR   BMRB; P22886; -.
DR   SMR; P22886; -.
DR   STRING; 1201292.DR75_1042; -.
DR   eggNOG; COG0582; Bacteria.
DR   EvolutionaryTrace; P22886; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008907; F:integrase activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR016177; DNA-bd_dom_sf.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004191; Integrase_Tn916-type_DNA-bd_N.
DR   Pfam; PF02920; Integrase_DNA; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF54171; SSF54171; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA integration; DNA recombination; DNA-binding;
KW   Transposable element; Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..405
FT                   /note="Transposase from transposon Tn916"
FT                   /id="PRO_0000197559"
FT   DOMAIN          79..163
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          186..392
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        225
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        264
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        343
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        346
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        369
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        379
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   STRAND          8..11
FT                   /evidence="ECO:0007829|PDB:1B69"
FT   STRAND          17..19
FT                   /evidence="ECO:0007829|PDB:1B69"
FT   STRAND          21..23
FT                   /evidence="ECO:0007829|PDB:1B69"
FT   STRAND          25..30
FT                   /evidence="ECO:0007829|PDB:1B69"
FT   STRAND          34..42
FT                   /evidence="ECO:0007829|PDB:1B69"
FT   HELIX           60..68
FT                   /evidence="ECO:0007829|PDB:1B69"
SQ   SEQUENCE   405 AA;  47050 MW;  33E7AF825154E619 CRC64;
     MSEKRRDNRG RILKTGESQR KDGRYLYKYI DSFGEPQFVY SWKLVATDRV PAGKRDCISL
     REKIAELQKD IHDGIDVVGK KMTLCQLYAK QNAQRPKVRK NTETGRKYLM DILKKDKLGV
     RSIDSIKPSD AKEWAIRMSE NGYAYQTINN YKRSLKASFY IAIQDDCVRK NPFDFQLKAV
     LDDDTVPKTV LTEEQEEKLL AFAKADKTYS KNYDEILILL KTGLRISEFG GLTLPDLDFE
     NRLVNIDHQL LRDTEIGYYI ETPKTKSGER QVPMVEEAYQ AFKRVLANRK NDKRVEIDGY
     SDFLFLNRKN YPKVASDYNG MMKGLVKKYN KYNEDKLPHI TPHSLRHTFC TNYANAGMNP
     KALQYIMGHA NIAMTLNYYA HATFDSAMAE MKRLNKEKQQ ERLVA