ID   TNRI_BACTU              Reviewed;         284 AA.
AC   P10020;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=TnP I resolvase;
GN   Name=tnpI;
OS   Bacillus thuringiensis.
OG   Plasmid pGI2.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H1.1;
RX   PubMed=3211758; DOI=10.1093/nar/16.24.11827;
RA   Mahillon J., Seurinck J.;
RT   "Complete nucleotide sequence of pGI2, a Bacillus thuringiensis plasmid
RT   containing Tn4430.";
RL   Nucleic Acids Res. 16:11827-11827(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H1.1; TRANSPOSON=Tn4430;
RX   PubMed=2842151; DOI=10.1002/j.1460-2075.1988.tb02971.x;
RA   Mahillon J., Lereclus D.;
RT   "Structural and functional analysis of Tn4430: identification of an
RT   integrase-like protein involved in the co-integrate-resolution process.";
RL   EMBO J. 7:1515-1526(1988).
CC   -!- FUNCTION: Resolvase catalyzes the resolution (a site-specific
CC       recombination) of the cointegrated replicon to yield the final
CC       transposition products.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X13481; CAA31832.1; -; Genomic_DNA.
DR   EMBL; X07651; CAA30491.1; -; Genomic_DNA.
DR   PIR; S00554; RSBSTI.
DR   RefSeq; WP_000382147.1; NZ_WJDT01000102.1.
DR   RefSeq; YP_001485222.1; NC_009841.1.
DR   AlphaFoldDB; P10020; -.
DR   SMR; P10020; -.
DR   PRIDE; P10020; -.
DR   GeneID; 67470162; -.
DR   OrthoDB; 745068at2; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF13495; Phage_int_SAM_4; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Transposable element.
FT   CHAIN           1..284
FT                   /note="TnP I resolvase"
FT                   /id="PRO_0000197549"
FT   DOMAIN          1..84
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          107..282
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        145
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        234
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        237
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        260
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        269
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   284 AA;  32820 MW;  7FAD9AA27B0FA745 CRC64;
     MDVAKQFSSY LKQENKTENT VQGYTSGIRQ YIKWFEGSYD RKLTKLYRQN ILEYISYLKN
     VKMLNAKSIN HKISSLAKFN EFLIQKGSQQ DQVILKTDMI KVQTVYASPT QIVELDVKKF
     LQSVLEDNNK RNYAIATLLA YTGVRISEAL SIKMNDFNLQ TGECIIRSGK GGKQRIVLLN
     SKVLSAIKDY LIDRKTYSTA HESPYLFISK KREKLDRTVV NRIFKSYSNV ITPHQLRHFF
     CTNAIEKGFS IHEVANQAGH SNIHTTLLYT NPNQLQLKNK MELL