ID   TNSB_ECOLX              Reviewed;         702 AA.
AC   P13989; Q9R5R8;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Transposon Tn7 transposition protein TnsB;
GN   Name=tnsB;
OS   Escherichia coli.
OG   Plasmid R721.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2156235; DOI=10.1093/nar/18.4.901;
RA   Flores C., Qadri M.I., Lichtenstein C.;
RT   "DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7
RT   transposition.";
RL   Nucleic Acids Res. 18:901-911(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=R721;
RA   Sampei G., Motomura K., Masuda S., Yamaguchi T., Ando K., Oishi T.,
RA   Furuya N., Komano T., Mizobuchi K.;
RT   "Organization and diversification of plasmid genomes: complete nucleotide
RT   sequence of the R721 genome.";
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86.
RX   PubMed=1655576; DOI=10.1016/0378-1119(91)90478-t;
RA   Orle K.A., Craig N.L.;
RT   "Identification of transposition proteins encoded by the bacterial
RT   transposon Tn7.";
RL   Gene 96:1-7(1990).
RN   [4]
RP   ERRATUM OF PUBMED:1655576.
RA   Orle K.A., Craig N.L.;
RL   Gene 104:125-131(1991).
RN   [5]
RP   PROTEIN SEQUENCE OF 1-12.
RX   PubMed=1657979; DOI=10.1016/s0021-9258(18)54698-6;
RA   Arciszewska L.K., McKown R.L., Craig N.L.;
RT   "Purification of TnsB, a transposition protein that binds to the ends of
RT   Tn7.";
RL   J. Biol. Chem. 266:21736-21744(1991).
RN   [6]
RP   FUNCTION, AND MUTAGENESIS OF ASP-273; ASP-278; ASP-283; ASP-301; SER-304;
RP   ASP-361; GLU-364; GLU-371; GLU-396 AND GLU-424.
RX   PubMed=8947057; DOI=10.1002/j.1460-2075.1996.tb01024.x;
RA   Sarnovsky R.J., May E.W., Craig N.L.;
RT   "The Tn7 transposase is a heteromeric complex in which DNA breakage and
RT   joining activities are distributed between different gene products.";
RL   EMBO J. 15:6348-6361(1996).
RN   [7]
RP   FUNCTION.
RX   PubMed=10704304; DOI=10.1006/jmbi.2000.3558;
RA   Biery M.C., Lopata M., Craig N.L.;
RT   "A minimal system for Tn7 transposition: the transposon-encoded proteins
RT   TnsA and TnsB can execute DNA breakage and joining reactions that generate
RT   circularized Tn7 species.";
RL   J. Mol. Biol. 297:25-37(2000).
CC   -!- FUNCTION: Sequence-specific, DNA-binding protein required for Tn7
CC       transposition. Recognizes sequences necessary for recombination at both
CC       left and right ends of Tn7 and, together with TnsA, forms the
CC       transposase. TnsB executes the 5'-DNA strand breakage and joining
CC       reactions. {ECO:0000269|PubMed:10704304, ECO:0000269|PubMed:8947057}.
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DR   EMBL; X17693; CAA35684.1; -; Genomic_DNA.
DR   EMBL; AP002527; BAB12612.1; -; Genomic_DNA.
DR   PIR; S12638; S12638.
DR   RefSeq; NP_065319.1; NC_002525.1.
DR   RefSeq; WP_000267723.1; NZ_WWEV01000116.1.
DR   AlphaFoldDB; P13989; -.
DR   BRENDA; 2.7.7.B22; 2026.
DR   GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004803; F:transposase activity; IDA:UniProtKB.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IDA:UniProtKB.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00665; rve; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA recombination; DNA-binding; Plasmid;
KW   Transposable element; Transposition.
FT   CHAIN           1..702
FT                   /note="Transposon Tn7 transposition protein TnsB"
FT                   /id="PRO_0000072612"
FT   DOMAIN          262..480
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   DNA_BIND        105..124
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          137..160
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          623..702
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        636..663
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         273
FT                   /note="D->N: Prevents DNA strand breakage and joining
FT                   reactions."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         278
FT                   /note="D->N: No effect on recombination."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         283
FT                   /note="D->N: No effect on recombination."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         301
FT                   /note="D->A: Prevents DNA strand breakage and joining
FT                   reactions."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         304
FT                   /note="S->A: No effect on recombination."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         361
FT                   /note="D->N: Prevents DNA strand breakage and joining
FT                   reactions."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         364
FT                   /note="E->A: DNA strand single-end joining."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         371
FT                   /note="E->A: No effect on recombination."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         396
FT                   /note="E->Q: Prevents DNA strand breakage and joining
FT                   reactions."
FT                   /evidence="ECO:0000269|PubMed:8947057"
FT   MUTAGEN         424
FT                   /note="E->A: No effect on recombination."
FT                   /evidence="ECO:0000269|PubMed:8947057"
SQ   SEQUENCE   702 AA;  80825 MW;  C71AE57F2DA1B0D8 CRC64;
     MWQINEVVLF DNDPYRILAI EDGQVVWMQI SADKGVPQAR AELLLMQYLD EGRLVRTDDP
     YVHLDLEEPS VDSVSFQKRE EDYRKILPII NSKDRFDPKV RSELVEHVVQ EHKVTKATVY
     KLLRRYWQRG QTPNALIPDY KNSGAPGERR SATGTAKIGR AREYGKGEGT KVTPEIERLF
     RLTIEKHLLN QKGTKTTVAY RRFVDLFAQY FPRIPQEDYP TLRQFRYFYD REYPKAQRLK
     SRVKAGVYKK DVRPLSSTAT SQALGPGSRY EIDATIADIY LVDHHDRQKI IGRPTLYIVI
     DVFSRMITGF YIGFENPSYV VAMQAFVNAC SDKTAICAQH DIEISSSDWP CVGLPDVLLA
     DRGELMSHQV EALVSSFNVR VESAPPRRGD AKGIVESTFR TLQAEFKSFA PGIVEGSRIK
     SHGETDYRLD ASLSVFEFTQ IILRTILFRN NHLVMDKYDR DADFPTDLPS IPVQLWQWGM
     QHRTGSLRAV EQEQLRVALL PRRKVSISSF GVNLWGLYYS GSEILREGWL QRSTDIARPQ
     HLEAAYDPVL VDTIYLFPQV GSRVFWRCNL TERSRQFKGL SFWEVWDIQA QEKHNKANAK
     QDELTKRREL EAFIQQTIQK ANKLTPSTTE PKSTRIKQIK TNKKEAVTSE RKKRAEHLKP
     SSSGDEAKVI PFNAVEADDQ EDYSLPTYVP ELFQDPPEKD ES