ID   TNSD_ECOLX              Reviewed;         508 AA.
AC   P13991; Q7AJH7;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Transposon Tn7 transposition protein TnsD;
GN   Name=tnsD;
OS   Escherichia coli.
OG   Plasmid R721.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2156235; DOI=10.1093/nar/18.4.901;
RA   Flores C., Qadri M.I., Lichtenstein C.;
RT   "DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7
RT   transposition.";
RL   Nucleic Acids Res. 18:901-911(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=R721;
RA   Sampei G., Motomura K., Masuda S., Yamaguchi T., Ando K., Oishi T.,
RA   Furuya N., Komano T., Mizobuchi K.;
RT   "Organization and diversification of plasmid genomes: complete nucleotide
RT   sequence of the R721 genome.";
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
RX   PubMed=1655576; DOI=10.1016/0378-1119(91)90478-t;
RA   Orle K.A., Craig N.L.;
RT   "Identification of transposition proteins encoded by the bacterial
RT   transposon Tn7.";
RL   Gene 96:1-7(1990).
RN   [4]
RP   ERRATUM OF PUBMED:1655576.
RA   Orle K.A., Craig N.L.;
RL   Gene 104:125-131(1991).
CC   -!- FUNCTION: Required for Tn7 transposition. It may at least play a role
CC       in DNA sequence recognition. It seems to be required for specific
CC       binding to a region of att-Tn7.
CC   -!- FUNCTION: TnsABC + TnsD promote high-frequency insertion of Tn7 into a
CC       specific target site known as att-Tn7 whereas TnsABC + TnsE promote
CC       low-frequency insertion into many different sites.
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DR   EMBL; X17693; CAA35686.1; -; Genomic_DNA.
DR   EMBL; AP002527; BAB12610.1; -; Genomic_DNA.
DR   EMBL; M37393; AAA24682.1; -; Genomic_DNA.
DR   PIR; S12640; S12640.
DR   RefSeq; NP_065317.1; NC_002525.1.
DR   RefSeq; WP_001243518.1; NZ_WWEV01000116.1.
DR   AlphaFoldDB; P13991; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   InterPro; IPR009492; TniQ.
DR   InterPro; IPR032750; TnsD_C.
DR   Pfam; PF06527; TniQ; 1.
DR   Pfam; PF15978; TnsD; 2.
PE   4: Predicted;
KW   DNA recombination; DNA-binding; Plasmid; Transposable element;
KW   Transposition.
FT   CHAIN           1..508
FT                   /note="Transposon Tn7 transposition protein TnsD"
FT                   /id="PRO_0000072614"
FT   DNA_BIND        222..241
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   508 AA;  59140 MW;  98FBF2D7561A8F70 CRC64;
     MRNFPVPYSN ELIYSTIARA GVYQGIVSPK QLLDEVYGNR KVVATLGLPS HLGVIARHLH
     QTGRYAVQQL IYEHTLFPLY APFVGKERRD EAIRLMEYQA QGAVHLMLGV AASRVKSDNR
     FRYCPDCVAL QLNRYGEAFW QRDWYLPALP YCPKHGALVF FDRAVDDHRH QFWALGHTEL
     LSDYPKDSLS QLTALAAYIA PLLDAPRAQE LSPSLEQWTL FYQRLAQDLG LTKSKHIRHD
     LVAERVRQTF SDEALEKLDL KLAENKDTCW LKSIFRKHRK AFSYLQHSIV WQALLPKLTV
     IEALQQASAL TEHSITTRPV SQSVQPNSED LSVKHKDWQQ LVHKYQGIKA ARQSLEGGVL
     YAWLYRHDRD WLVHWNQQHQ QERLAPAPRV DWNQRDRIAV RQLLRIIKRL DSSLDHPRAT
     SSWLLKQTPN GTSLAKNLQK LPLVALCLKR YSESVEDYQI RRISQAFIKL KQEDVELRRW
     RLLRSATLSK ERITEEAQRF LEMVYGEE