ID   TNSE_ECOLX              Reviewed;         538 AA.
AC   P05845; Q7AJH8;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Transposon Tn7 transposition protein TnsE;
DE   AltName: Full=Protein D;
GN   Name=tnsE;
OS   Escherichia coli.
OG   Plasmid R721.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2156235; DOI=10.1093/nar/18.4.901;
RA   Flores C., Qadri M.I., Lichtenstein C.;
RT   "DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7
RT   transposition.";
RL   Nucleic Acids Res. 18:901-911(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3022239; DOI=10.1093/nar/14.20.7915;
RA   Smith G.M., Jones P.;
RT   "Tn7 transposition: a multigene process. Identification of a regulatory
RT   gene product.";
RL   Nucleic Acids Res. 14:7915-7927(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=R721;
RA   Sampei G., Motomura K., Masuda S., Yamaguchi T., Ando K., Oishi T.,
RA   Furuya N., Komano T., Mizobuchi K.;
RT   "Organization and diversification of plasmid genomes: complete nucleotide
RT   sequence of the R721 genome.";
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: TnsABC + TnsD promote high-frequency insertion of Tn7 into a
CC       specific target site known as att-Tn7 whereas TnsABC + TnsE promote
CC       low-frequency insertion into many different sites.
CC   -!- INTERACTION:
CC       P05845; P0A988: dnaN; Xeno; NbExp=4; IntAct=EBI-2434514, EBI-542385;
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DR   EMBL; X17693; CAA35687.1; -; Genomic_DNA.
DR   EMBL; X04534; CAB56509.1; -; Genomic_DNA.
DR   EMBL; AP002527; BAB12609.1; -; Genomic_DNA.
DR   PIR; A25543; QQECD7.
DR   RefSeq; NP_065316.1; NC_002525.1.
DR   RefSeq; WP_000251879.1; NZ_WWEV01000116.1.
DR   PDB; 5D16; X-ray; 1.76 A; A/B=342-538.
DR   PDB; 5D17; X-ray; 2.85 A; A/B/C/D/E/F/G/H/I/K/L/M=342-538.
DR   PDBsum; 5D16; -.
DR   PDBsum; 5D17; -.
DR   AlphaFoldDB; P05845; -.
DR   SMR; P05845; -.
DR   IntAct; P05845; 1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   InterPro; IPR041419; TnsE_C.
DR   InterPro; IPR016421; Transposition_TnsE.
DR   Pfam; PF18623; TnsE_C; 1.
DR   PIRSF; PIRSF004567; Transposition_TnsE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA recombination; DNA-binding; Plasmid;
KW   Transposable element; Transposition.
FT   CHAIN           1..538
FT                   /note="Transposon Tn7 transposition protein TnsE"
FT                   /id="PRO_0000072615"
FT   DNA_BIND        311..330
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          330..360
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        343..359
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           383..389
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   HELIX           390..402
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   STRAND          406..414
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   STRAND          429..431
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   STRAND          433..441
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   STRAND          444..451
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   STRAND          462..467
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   TURN            470..472
FT                   /evidence="ECO:0007829|PDB:5D17"
FT   HELIX           473..486
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   HELIX           493..500
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   HELIX           502..504
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   STRAND          505..508
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   TURN            512..515
FT                   /evidence="ECO:0007829|PDB:5D16"
FT   HELIX           521..532
FT                   /evidence="ECO:0007829|PDB:5D16"
SQ   SEQUENCE   538 AA;  61212 MW;  DE34A3F141A1885B CRC64;
     MVRLATFNDN VQVVHIGHLF RNSGHKEWRI FVWFNPMQER KWTRFTHLPL LSRAKVVNST
     TKQINKADRV IEFEASDLQR AKIIDFPNLS SFASVRNKDG AQSSFIYEAE TPYSKTRYHI
     PQLELARSLF LINSYFCRSC LSSTALQQEF DVQYEVERDH LEIRILPSSS FPKGALEQSA
     VVQLLVWLFS DQDVMDSYES IFRHYQQNRE IKNGVESWCF SFDPPPMQGW KLHVKGRSSN
     EDKDYLVEEI VGLEINAMLP STTAISHASF QEKEAGDGST QHIAVSTESV VDDEHLQLDD
     EETANIDTDT RVIEAEPTWI SFSRPSRIEK SRRARKSSQT ILEKEEATTS ENSNLVSTDE
     PHLGGVLAAA DVGGKQDATN YNSIFANRFA AFDELLSILK TKFACRVLFE ETLVLPKVGR
     SRLHLCKDGS PRVIKAVGVQ RNGSEFVLLE VDASDGVKML STKVLSGVDS ETWRNDFEKI
     RRGVVKSSLN WPNSLFDQLY GQDGHRGVNH PKGLGELQVS REDMEGWAER VVREQFTH