ID   TO1F_HADVE              Reviewed;          37 AA.
AC   P81599;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Omega-hexatoxin-Hv1f {ECO:0000305};
DE            Short=Omega-HXTX-Hv1f {ECO:0000305};
DE   AltName: Full=Omega-atracotoxin-Hv1f {ECO:0000303|PubMed:10491095};
DE            Short=Omega-AcTx-Hv1f {ECO:0000303|PubMed:10491095};
OS   Hadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Hexathelidae; Hadronyche.
OX   NCBI_TaxID=6904;
RN   [1]
RP   PROTEIN SEQUENCE, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=10491095; DOI=10.1046/j.1432-1327.1999.00646.x;
RA   Wang X.-H., Smith R., Fletcher J.I., Wilson H., Wood C.J., Merlin E.H.,
RA   King G.F.;
RT   "Structure-function studies of omega-atracotoxin, a potent antagonist of
RT   insect voltage-gated calcium channels.";
RL   Eur. J. Biochem. 264:488-494(1999).
CC   -!- FUNCTION: Inhibits insect, but not mammalian, voltage-gated calcium
CC       channels (Cav). {ECO:0000250|UniProtKB:P56207}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10491095}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:10491095}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P56207}.
CC   -!- TOXIC DOSE: LD(50) is 1384 pmol/g when injected into house crickets
CC       (Acheta domestica). {ECO:0000269|PubMed:10491095}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 08 (Shiva) family. 01 (omega
CC       toxin) subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P81599; -.
DR   SMR; P81599; -.
DR   ArachnoServer; AS000201; omega-hexatoxin-Hv1f.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019855; F:calcium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   InterPro; IPR009415; Omega-atracotox.
DR   InterPro; IPR018071; Omega-atracotox_CS.
DR   Pfam; PF06357; Omega-toxin; 1.
DR   PROSITE; PS60016; OMEGA_ACTX_1; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   PEPTIDE         1..37
FT                   /note="Omega-hexatoxin-Hv1f"
FT                   /evidence="ECO:0000269|PubMed:10491095"
FT                   /id="PRO_0000044993"
FT   SITE            10
FT                   /note="Critical for insecticidal activity"
FT                   /evidence="ECO:0000250|UniProtKB:P56207"
FT   SITE            27
FT                   /note="Critical for insecticidal activity"
FT                   /evidence="ECO:0000250|UniProtKB:P56207"
FT   SITE            35
FT                   /note="Critical for insecticidal activity"
FT                   /evidence="ECO:0000250|UniProtKB:P56207"
FT   DISULFID        4..18
FT                   /evidence="ECO:0000250|UniProtKB:P56207"
FT   DISULFID        11..22
FT                   /evidence="ECO:0000250|UniProtKB:P56207"
FT   DISULFID        17..36
FT                   /evidence="ECO:0000250|UniProtKB:P56207"
SQ   SEQUENCE   37 AA;  3951 MW;  EEC16AE67EE3F36A CRC64;
     SAVCIPSGQP CPYSKYCCSG SCTYKTNENG NSVQRCD