TO201_ARATH
ID TO201_ARATH Reviewed; 188 AA.
AC P82872; Q9LSC9;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Mitochondrial import receptor subunit TOM20-1;
DE AltName: Full=Translocase of outer membrane 20 kDa subunit 1;
GN Name=TOM20-1; OrderedLocusNames=At3g27070; ORFNames=MOJ10.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11161051; DOI=10.1104/pp.125.2.943;
RA Werhahn W., Niemeyer A., Jaensch L., Kruft V., Schmitz U.K., Braun H.-P.;
RT "Purification and characterization of the preprotein translocase of the
RT outer mitochondrial membrane from Arabidopsis. Identification of multiple
RT forms of TOM20.";
RL Plant Physiol. 125:943-954(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP SUBUNIT.
RA Werhahn W., Jaensch L., Braun H.-P.;
RT "Identification of novel subunits of the TOM complex from Arabidopsis
RT thaliana.";
RL Plant Physiol. Biochem. 41:407-416(2003).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=17981999; DOI=10.1105/tpc.107.050534;
RA Lister R., Carrie C., Duncan O., Ho L.H., Howell K.A., Murcha M.W.,
RA Whelan J.;
RT "Functional definition of outer membrane proteins involved in preprotein
RT import into mitochondria.";
RL Plant Cell 19:3739-3759(2007).
CC -!- FUNCTION: Central component of the receptor complex responsible for the
CC recognition and translocation of cytosolically synthesized
CC mitochondrial preproteins. Together with TOM22 functions as the transit
CC peptide receptor at the surface of the mitochondrion outer membrane and
CC facilitates the movement of preproteins into the translocation pore.
CC {ECO:0000269|PubMed:17981999}.
CC -!- SUBUNIT: Forms part of the preprotein translocase complex of the outer
CC mitochondrial membrane (TOM complex) which consists of at least 6
CC different proteins (TOM5, TOM6, TOM7, TOM20, TOM22/TOM9 and TOM40).
CC {ECO:0000269|PubMed:17981999, ECO:0000269|Ref.4}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P82872-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Barely detected in roots.
CC {ECO:0000269|PubMed:17981999}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:17981999}.
CC -!- MISCELLANEOUS: There are four genes (TOM20-1, TOM20-2, TOM20-3 and
CC TOM20-4) which encode mitochondrial import receptor subunits TOM20, but
CC TOM20-1 is the only one that has not been directly identified in
CC isolated mitochondria from cv. Columbia.
CC -!- MISCELLANEOUS: In mammals and fungi, the transmembrane domain is
CC located at the N-terminus while it is located at the C-terminus in
CC plants. The overall orientation of the protein in the membrane is
CC therefore inverted.
CC -!- SIMILARITY: Belongs to the Tom20 family. {ECO:0000305}.
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DR EMBL; AJ296023; CAC17150.1; -; mRNA.
DR EMBL; AB026649; BAB01088.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77262.1; -; Genomic_DNA.
DR RefSeq; NP_189343.1; NM_113621.3. [P82872-1]
DR AlphaFoldDB; P82872; -.
DR SMR; P82872; -.
DR PaxDb; P82872; -.
DR PRIDE; P82872; -.
DR ProteomicsDB; 232404; -. [P82872-1]
DR EnsemblPlants; AT3G27070.1; AT3G27070.1; AT3G27070. [P82872-1]
DR GeneID; 822325; -.
DR Gramene; AT3G27070.1; AT3G27070.1; AT3G27070. [P82872-1]
DR KEGG; ath:AT3G27070; -.
DR Araport; AT3G27070; -.
DR eggNOG; ENOG502QT42; Eukaryota.
DR HOGENOM; CLU_117357_0_0_1; -.
DR InParanoid; P82872; -.
DR OMA; AEATYTK; -.
DR PhylomeDB; P82872; -.
DR PRO; PR:P82872; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P82872; baseline and differential.
DR Genevisible; P82872; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IEA:InterPro.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IEA:InterPro.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR010547; TOM20_imprt_rcpt.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR32409; PTHR32409; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..188
FT /note="Mitochondrial import receptor subunit TOM20-1"
FT /id="PRO_0000051543"
FT TOPO_DOM 1..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..188
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
SQ SEQUENCE 188 AA; 21305 MW; BE6EA6C0CB7C39BC CRC64;
MDKLNFFEEI RKDAEETYKL NPEDADNLMR WGEALLELSQ FQNVIDSLKM IQDAISKLED
AILIDPMKHD AVWCLGNAYT SYARLTPDDT QARLNFGLAY LFFGIAVAQQ PDNQVYHKSL
EMADKAPQLH TGFHKNRLLS LLGGVETLAI PSPKVVKNKK SSDEKYIVMG WVILAIGVVA
CISFRKLR
