ID   TO402_ARATH             Reviewed;         310 AA.
AC   Q9SX55;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 3.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Probable mitochondrial import receptor subunit TOM40-2;
DE   AltName: Full=Translocase of outer membrane 40 kDa subunit homolog 2;
GN   Name=TOM40-2; OrderedLocusNames=At1g50400; ORFNames=F14I3.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=14730085; DOI=10.1104/pp.103.033910;
RA   Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA   Millar A.H., Whelan J.;
RT   "A transcriptomic and proteomic characterization of the Arabidopsis
RT   mitochondrial protein import apparatus and its response to mitochondrial
RT   dysfunction.";
RL   Plant Physiol. 134:777-789(2004).
RN   [4]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA   Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA   Giglione C.;
RT   "Comparative large-scale characterisation of plant vs. mammal proteins
RT   reveals similar and idiosyncratic N-alpha acetylation features.";
RL   Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC   -!- FUNCTION: Central component of the receptor complex responsible for the
CC       recognition and translocation of cytosolically synthesized
CC       mitochondrial preproteins. Together with TOM22 functions as the transit
CC       peptide receptor at the surface of the mitochondrion outer membrane and
CC       facilitates the movement of preproteins into the translocation pore.
CC       Directly involved in the pore formation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms part of the preprotein translocase complex of the outer
CC       mitochondrial membrane (TOM complex) which consists of at least 6
CC       different proteins (TOM5, TOM6, TOM7, TOM20, TOM22/TOM9 and TOM40).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, flowers, young cotyledons and
CC       leaves. {ECO:0000269|PubMed:14730085}.
CC   -!- INDUCTION: Up-regulated after antimycin A or rotenone treatments.
CC       {ECO:0000269|PubMed:14730085}.
CC   -!- SIMILARITY: Belongs to the Tom40 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD50049.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC007980; AAD50049.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE32544.1; -; Genomic_DNA.
DR   PIR; C96540; C96540.
DR   RefSeq; NP_175457.1; NM_103923.3.
DR   AlphaFoldDB; Q9SX55; -.
DR   SMR; Q9SX55; -.
DR   STRING; 3702.AT1G50400.1; -.
DR   TCDB; 1.B.8.2.3; the mitochondrial and plastid porin (mpp) family.
DR   iPTMnet; Q9SX55; -.
DR   PaxDb; Q9SX55; -.
DR   PRIDE; Q9SX55; -.
DR   ProteomicsDB; 234620; -.
DR   DNASU; 841462; -.
DR   EnsemblPlants; AT1G50400.1; AT1G50400.1; AT1G50400.
DR   GeneID; 841462; -.
DR   Gramene; AT1G50400.1; AT1G50400.1; AT1G50400.
DR   KEGG; ath:AT1G50400; -.
DR   Araport; AT1G50400; -.
DR   TAIR; locus:2011887; AT1G50400.
DR   eggNOG; KOG3296; Eukaryota.
DR   HOGENOM; CLU_042174_1_0_1; -.
DR   InParanoid; Q9SX55; -.
DR   OMA; MNLPCPV; -.
DR   OrthoDB; 1346842at2759; -.
DR   PhylomeDB; Q9SX55; -.
DR   PRO; PR:Q9SX55; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SX55; baseline and differential.
DR   Genevisible; Q9SX55; AT.
DR   GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   CDD; cd07305; Porin3_Tom40; 1.
DR   Gene3D; 2.40.160.10; -; 1.
DR   InterPro; IPR023614; Porin_dom_sf.
DR   InterPro; IPR027246; Porin_Euk/Tom40.
DR   InterPro; IPR037930; Tom40.
DR   PANTHER; PTHR10802; PTHR10802; 1.
DR   Pfam; PF01459; Porin_3; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Ion transport; Membrane; Mitochondrion;
KW   Mitochondrion outer membrane; Porin; Protein transport; Reference proteome;
KW   Transmembrane; Transmembrane beta strand; Transport.
FT   CHAIN           1..310
FT                   /note="Probable mitochondrial import receptor subunit
FT                   TOM40-2"
FT                   /id="PRO_0000051532"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0007744|PubMed:22223895"
SQ   SEQUENCE   310 AA;  34473 MW;  3164DB4098094AC8 CRC64;
     MEGFSPPINT AQVDAKTKLD EKVDYSNLPC PVLYEELNRE ATMALKPELF EGFRLDYNKS
     LNQKFFLSHS ILMGPTEVPN PTPSSEIIKI PTANYDFGAG FIDPKLYLIG RITTDGRLNA
     RAKFDLTDNF SVKANALLTD EEDKSQGHLV IDYKGSDYRT QLQLGNNSVY AANYIQHVTP
     HLSLGGEAFW LGQQLMSGVG YAARYETDKT VASGQIASTG VAVMNYVHKV SEKLSFATDF
     IYNYLSRDVT ASVGYDLITR QSRLRGKVDS NGVVAAYLEE QLPIGLRFLL SAEVDHVKKD
     YKFGFGVNAF