TO6BL_ARATH
ID TO6BL_ARATH Reviewed; 473 AA.
AC Q5Q0E6; O22692; Q5Q0E4; Q5Q0E5;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Type 2 DNA topoisomerase 6 subunit B-like {ECO:0000305};
DE AltName: Full=Meiotic topoisomerase VIB-like {ECO:0000303|PubMed:26917763};
DE Short=AtMTOPVIB {ECO:0000303|PubMed:26917764};
GN Name=MTOPVIB {ECO:0000303|PubMed:26917763};
GN OrderedLocusNames=At1g60460 {ECO:0000312|Araport:AT1G60460};
GN ORFNames=F8A5.1 {ECO:0000312|EMBL:AAB71954.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPO11-1 AND SPO11-2,
RP DOMAIN, AND DISRUPTION PHENOTYPE.
RX PubMed=26917763; DOI=10.1126/science.aad5196;
RA Vrielynck N., Chambon A., Vezon D., Pereira L., Chelysheva L., De Muyt A.,
RA Mezard C., Mayer C., Grelon M.;
RT "A DNA topoisomerase VI-like complex initiates meiotic recombination.";
RL Science 351:939-943(2016).
RN [6]
RP IDENTIFICATION.
RX PubMed=26917764; DOI=10.1126/science.aad5309;
RA Robert T., Nore A., Brun C., Maffre C., Crimi B., Bourbon H.M.,
RA de Massy B.;
RT "The TopoVIB-Like protein family is required for meiotic DNA double-strand
RT break formation.";
RL Science 351:943-949(2016).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PRD1; SPO11-1 AND SPO11-2,
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=28855712; DOI=10.1038/s41598-017-10270-9;
RA Tang Y., Yin Z., Zeng Y., Zhang Q., Chen L., He Y., Lu P., Ye D., Zhang X.;
RT "MTOPVIB interacts with AtPRD1 and plays important roles in formation of
RT meiotic DNA double-strand breaks in Arabidopsis.";
RL Sci. Rep. 7:10007-10007(2017).
CC -!- FUNCTION: Component of a topoisomerase 6 complex specifically required
CC for meiotic recombination (PubMed:26917763, PubMed:28855712). Together
CC with SPO11 (SPO11-1 and SPO11-2), mediates DNA cleavage that forms the
CC double-strand breaks (DSB) that initiate meiotic recombination
CC (PubMed:26917763, PubMed:28855712). The complex promotes relaxation of
CC negative and positive supercoiled DNA and DNA decatenation through
CC cleavage and ligation cycles (PubMed:26917763).
CC {ECO:0000269|PubMed:26917763, ECO:0000269|PubMed:28855712}.
CC -!- SUBUNIT: Heterotetramer of 2 SPO11 (SPO11-1 and/or SPO11-2) and 2
CC MTOPVIB chains (Probable). Interacts with SPO11-1 and SPO11-2
CC (PubMed:26917763, PubMed:28855712). Binds to PRD1 (PubMed:28855712).
CC {ECO:0000269|PubMed:26917763, ECO:0000269|PubMed:28855712,
CC ECO:0000305}.
CC -!- INTERACTION:
CC Q5Q0E6; Q9M4A2: SPO11-1; NbExp=6; IntAct=EBI-16200362, EBI-1540725;
CC Q5Q0E6; Q9M4A1: SPO11-2; NbExp=7; IntAct=EBI-16200362, EBI-1772309;
CC Q5Q0E6; Q9LZ03: TOP6A; NbExp=4; IntAct=EBI-16200362, EBI-1772104;
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:26917763}. Nucleus
CC {ECO:0000269|PubMed:28855712}. Cytoplasm {ECO:0000269|PubMed:28855712}.
CC Note=Localizes to meiotic chromosomes from leptotene to pachytene
CC (PubMed:26917763). Mostly present in the nucleus, but also present in
CC the cytoplasm (PubMed:28855712). {ECO:0000269|PubMed:26917763,
CC ECO:0000269|PubMed:28855712}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5Q0E6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5Q0E6-2; Sequence=VSP_058338;
CC Name=3;
CC IsoId=Q5Q0E6-3; Sequence=VSP_058339;
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously in different tissues,
CC including roots, stems, leaves, seedlings, inflorescences, siliques and
CC pollen grains, specially as a higher level in inflorescence.
CC {ECO:0000269|PubMed:28855712}.
CC -!- DOMAIN: Despite a weak sequence similarity, retains most of the
CC structural features of the ancestral archaeal Top6B subunit (AC
CC O05207), including the transducer domain that interacts with the SPO11
CC subunit and the ATP-binding fold, also named GHKL fold.
CC {ECO:0000269|PubMed:26917763}.
CC -!- DISRUPTION PHENOTYPE: Defective in meiosis (PubMed:28855712). Sterility
CC due to male and female gamete developmental defects leading to
CC disturbed embryo sac development and abnormal pollen formation
CC (PubMed:26917763, PubMed:28855712). Strong reduction in bivalent
CC formation at metaphase I caused by defects in meiotic double-strand
CC breaks (DSB) formation (PubMed:26917763, PubMed:28855712). Impaired
CC homologous chromosome synapsis and recombination (PubMed:28855712).
CC {ECO:0000269|PubMed:26917763, ECO:0000269|PubMed:28855712}.
CC -!- SIMILARITY: Belongs to the TOP6B-like family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB71954.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002292; AAB71954.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33689.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33690.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33691.1; -; Genomic_DNA.
DR EMBL; AY800605; AAV68841.1; -; mRNA.
DR EMBL; AY800606; AAV68842.1; -; mRNA.
DR EMBL; AY800607; AAV68843.1; -; mRNA.
DR EMBL; DQ653404; ABK28783.1; -; mRNA.
DR PIR; G96629; G96629.
DR RefSeq; NP_001031210.1; NM_001036133.1. [Q5Q0E6-2]
DR RefSeq; NP_001031211.1; NM_001036134.1. [Q5Q0E6-1]
DR RefSeq; NP_176249.2; NM_104733.4. [Q5Q0E6-3]
DR AlphaFoldDB; Q5Q0E6; -.
DR DIP; DIP-62003N; -.
DR IntAct; Q5Q0E6; 3.
DR STRING; 3702.AT1G60460.1; -.
DR PaxDb; Q5Q0E6; -.
DR PRIDE; Q5Q0E6; -.
DR EnsemblPlants; AT1G60460.1; AT1G60460.1; AT1G60460. [Q5Q0E6-3]
DR EnsemblPlants; AT1G60460.2; AT1G60460.2; AT1G60460. [Q5Q0E6-2]
DR EnsemblPlants; AT1G60460.3; AT1G60460.3; AT1G60460. [Q5Q0E6-1]
DR GeneID; 842341; -.
DR Gramene; AT1G60460.1; AT1G60460.1; AT1G60460. [Q5Q0E6-3]
DR Gramene; AT1G60460.2; AT1G60460.2; AT1G60460. [Q5Q0E6-2]
DR Gramene; AT1G60460.3; AT1G60460.3; AT1G60460. [Q5Q0E6-1]
DR KEGG; ath:AT1G60460; -.
DR Araport; AT1G60460; -.
DR TAIR; locus:2036525; AT1G60460.
DR eggNOG; ENOG502QSZ1; Eukaryota.
DR HOGENOM; CLU_044922_0_0_1; -.
DR OMA; DFIPCPI; -.
DR OrthoDB; 681109at2759; -.
DR PhylomeDB; Q5Q0E6; -.
DR PRO; PR:Q5Q0E6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q5Q0E6; baseline and differential.
DR GO; GO:0000793; C:condensed chromosome; IMP:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; TAS:UniProtKB.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IPI:TAIR.
DR GO; GO:0048314; P:embryo sac morphogenesis; IMP:UniProtKB.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:UniProtKB.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; IDA:TAIR.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR InterPro; IPR034566; MTOPVIB_plant.
DR PANTHER; PTHR36722; PTHR36722; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Cytoplasm; Meiosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..473
FT /note="Type 2 DNA topoisomerase 6 subunit B-like"
FT /id="PRO_0000436278"
FT VAR_SEQ 1..21
FT /note="MENNAPVPKLLLQLISSAFQR -> MLRFRSFFYK (in isoform 2)"
FT /id="VSP_058338"
FT VAR_SEQ 465..473
FT /note="GEYYSSSLE -> DRENSTHEYDATATKAYTQFKCLATMLQE (in
FT isoform 3)"
FT /id="VSP_058339"
SQ SEQUENCE 473 AA; 53344 MW; 509E9B3E2EA77328 CRC64;
MENNAPVPKL LLQLISSAFQ RCRLAEDLCR LSVLLDQSTE RDPPITCISI ADTGIGCNLE
EFQNLRCPRE FNGAKIWDGL LSVKTTCFTD DEVYYYHINL DEYIANKRLK RQPSQAKNGA
KFSGTEVSLS VFGSMDVLVA PIIGFFQKII VLQILNVTLD LMVKQGTSPG NQTQYVFAVN
ADKTPCFTAS NLERLKSGLE DYVLRHANCL DTMCDYCFSD REHLKVGSGT VCQEDKHKRV
GGTMEVVIVI SDLLESTQHC SRSCNGKTEV LYFDNFLPSP VPHLALSALK KIDWKKYGLI
LANVNDQDGH VFLEWDNFPS YVQIQIALHW YHNQYPTRQK NGPGISLLKK GIKNALDNLK
AKHEGFLLSS HSRKICSYVP DLARSIAGLI FSSTDLDFQG DCLSVLGFQT QEVERDTVEN
YIQRKIVTVI GMNERKPQKD QEAAPFLFFD GESETSFFED EEVEGEYYSS SLE
