TO6BL_DROME
ID TO6BL_DROME Reviewed; 331 AA.
AC Q9VS36; C9QPF9; Q9U626;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Meiotic recombination protein P22 {ECO:0000303|PubMed:12242237};
GN Name=mei-P22 {ECO:0000303|PubMed:12242237}; ORFNames=CG14827;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP INDUCTION.
RX PubMed=12242237; DOI=10.1093/genetics/162.1.245;
RA Liu H., Jang J.K., Kato N., McKim K.S.;
RT "mei-P22 encodes a chromosome-associated protein required for the
RT initiation of meiotic recombination in Drosophila melanogaster.";
RL Genetics 162:245-258(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.E.;
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION.
RX PubMed=26917764; DOI=10.1126/science.aad5309;
RA Robert T., Nore A., Brun C., Maffre C., Crimi B., Bourbon H.M.,
RA de Massy B.;
RT "The TopoVIB-Like protein family is required for meiotic DNA double-strand
RT break formation.";
RL Science 351:943-949(2016).
CC -!- FUNCTION: Required for formation of the mei-W68-mediated double-strand
CC breaks (DSBs) that initiate meiotic recombination.
CC {ECO:0000269|PubMed:12242237}.
CC -!- INTERACTION:
CC Q9VS36; P29310-2: 14-3-3zeta; NbExp=3; IntAct=EBI-102811, EBI-198120;
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:12242237}.
CC Note=Localizes to foci on meiotic chromosomes.
CC {ECO:0000269|PubMed:12242237}.
CC -!- INDUCTION: Expressed during early meiotic prophase.
CC {ECO:0000269|PubMed:12242237}.
CC -!- MISCELLANEOUS: Despite weak sequence similarities, may correspond to
CC the subunit B of a mei-W68/spo11-containing topoisomerase 6 complex
CC specifically required for meiotic recombination. Retains some of the
CC structural features of the ancestral archaeal Top6B subunit (AC
CC O05207). {ECO:0000305|PubMed:26917764}.
CC -!- SIMILARITY: Belongs to the TOP6B-like family. {ECO:0000305}.
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DR EMBL; AF199369; AAF09580.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF50593.2; -; Genomic_DNA.
DR EMBL; BT100073; ACX61614.3; -; mRNA.
DR RefSeq; NP_523956.1; NM_079232.1.
DR AlphaFoldDB; Q9VS36; -.
DR IntAct; Q9VS36; 10.
DR STRING; 7227.FBpp0076547; -.
DR PaxDb; Q9VS36; -.
DR DNASU; 38797; -.
DR EnsemblMetazoa; FBtr0076836; FBpp0076547; FBgn0016036.
DR GeneID; 38797; -.
DR KEGG; dme:Dmel_CG14827; -.
DR UCSC; CG14827-RA; d. melanogaster.
DR CTD; 38797; -.
DR FlyBase; FBgn0016036; mei-P22.
DR VEuPathDB; VectorBase:FBgn0016036; -.
DR eggNOG; ENOG502T97F; Eukaryota.
DR HOGENOM; CLU_861255_0_0_1; -.
DR InParanoid; Q9VS36; -.
DR OMA; LTMQRPR; -.
DR OrthoDB; 1156229at2759; -.
DR PhylomeDB; Q9VS36; -.
DR SignaLink; Q9VS36; -.
DR BioGRID-ORCS; 38797; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38797; -.
DR PRO; PR:Q9VS36; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0016036; Expressed in egg cell and 1 other tissue.
DR ExpressionAtlas; Q9VS36; baseline and differential.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Chromosome; Reference proteome.
FT CHAIN 1..331
FT /note="Meiotic recombination protein P22"
FT /id="PRO_0000436508"
FT REGION 132..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 148..164
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 4
FT /note="T -> A (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="P -> S (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="I -> T (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="S -> N (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
FT CONFLICT 200..212
FT /note="Missing (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
FT CONFLICT 253
FT /note="F -> I (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
FT CONFLICT 331
FT /note="P -> S (in Ref. 1; AAF09580)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 331 AA; 37365 MW; 89A01C2E2D4E2D69 CRC64;
MDRTTVVPSP ASTYCSFMGG SQHSPLRRSQ RLIDKENRDL QKIPPKSLSP QLFGNTEDDY
SELLLQQGKV VHLKPAAKLV PVRSSDNLVH LGSRDKVVPL KTSNRIVHLK PPYKIIHLKS
LDEVEKTVKK KNNIQKEVHQ RNSQRRSIQC TPKKRGRKPK QPAKKLQSRI STDQLGSTPS
PSKLPAKYPA HFVHQLPPAE DSSCATRRRS LSAPAMQLSD LSLTPDEIQT PERRGEIVNK
SPSDLDSDVS LNFSLSDSIG EIFGTKDISS ILTMQRPRQY ILLEEHLPTL ATMLNVDLER
LRCVLDITQG LSHEQILRFP IKHEEDELEV P
