TO6BL_MOUSE
ID TO6BL_MOUSE Reviewed; 579 AA.
AC J3QMY9; Q3UY62;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 11-MAY-2016, sequence version 2.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=Type 2 DNA topoisomerase 6 subunit B-like {ECO:0000303|PubMed:26917764};
DE AltName: Full=Type 2 DNA topoisomerase VI subunit B-like {ECO:0000303|PubMed:26917764};
DE Short=TOPOVIBL {ECO:0000303|PubMed:26917764};
GN Name=Top6bl {ECO:0000303|PubMed:26917764};
GN Synonyms=Gm960 {ECO:0000312|MGI:MGI:2685806};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DOMAIN, INTERACTION WITH SPO11, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=26917764; DOI=10.1126/science.aad5309;
RA Robert T., Nore A., Brun C., Maffre C., Crimi B., Bourbon H.M.,
RA de Massy B.;
RT "The TopoVIB-Like protein family is required for meiotic DNA double-strand
RT break formation.";
RL Science 351:943-949(2016).
CC -!- FUNCTION: Component of a topoisomerase 6 complex specifically required
CC for meiotic recombination. Together with SPO11, mediates DNA cleavage
CC that forms the double-strand breaks (DSB) that initiate meiotic
CC recombination. The complex promotes relaxation of negative and positive
CC supercoiled DNA and DNA decatenation through cleavage and ligation
CC cycles. {ECO:0000269|PubMed:26917764}.
CC -!- SUBUNIT: Heterotetramer of SPO11 and 2 TOP6BL chains (Probable).
CC Interacts with SPO11 (PubMed:26917764). {ECO:0000269|PubMed:26917764,
CC ECO:0000305}.
CC -!- INTERACTION:
CC J3QMY9; Q9WTK8-1: Spo11; NbExp=6; IntAct=EBI-16200997, EBI-16201014;
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000305}. Note=Localizes to
CC meiotic chromosomes. {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=J3QMY9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=J3QMY9-2; Sequence=VSP_058336, VSP_058337;
CC -!- TISSUE SPECIFICITY: In males, expressed in testis but not in other
CC tissues. Expressed in testis from 8 days postpartum (dpp) onward, with
CC a peak of expression around 12 to 14 dpp, at the beginning of meiotic
CC prophase. In females, expressed in embryonic ovaries when oocytes
CC proceed through meiotic prophase (PubMed:26917764).
CC {ECO:0000269|PubMed:26917764}.
CC -!- DOMAIN: Despite a weak sequence similarity, retains most of the
CC structural features of the ancestral archaeal Top6B subunit (AC
CC O05207), including the transducer domain that interacts with the SPO11
CC subunit and the ATP-binding fold, also named GHKL fold.
CC {ECO:0000269|PubMed:26917764}.
CC -!- DISRUPTION PHENOTYPE: Male mice develop normally and reach adulthood
CC but have smaller testes. Spermatogonia are present at the periphery of
CC tubules but are strongly depleted and spermatids are absent, due to
CC defects during meiotic prophase. {ECO:0000269|PubMed:26917764}.
CC -!- SIMILARITY: Belongs to the TOP6B-like family. {ECO:0000305}.
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DR EMBL; AK134945; BAE22351.1; -; mRNA.
DR EMBL; AC128739; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC147618; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; J3QMY9; -.
DR DIP; DIP-62005N; -.
DR IntAct; J3QMY9; 1.
DR STRING; 10090.ENSMUSP00000136515; -.
DR PhosphoSitePlus; J3QMY9; -.
DR PaxDb; J3QMY9; -.
DR PRIDE; J3QMY9; -.
DR ProteomicsDB; 258948; -. [J3QMY9-2]
DR Antibodypedia; 52536; 16 antibodies from 8 providers.
DR Ensembl; ENSMUST00000096325; ENSMUSP00000094049; ENSMUSG00000071691. [J3QMY9-2]
DR Ensembl; ENSMUST00000225896; ENSMUSP00000153439; ENSMUSG00000071691. [J3QMY9-1]
DR UCSC; uc033hik.1; mouse. [J3QMY9-1]
DR MGI; MGI:2685806; Gm960.
DR VEuPathDB; HostDB:ENSMUSG00000071691; -.
DR eggNOG; ENOG502S5QP; Eukaryota.
DR GeneTree; ENSGT00390000009327; -.
DR HOGENOM; CLU_369584_0_0_1; -.
DR InParanoid; J3QMY9; -.
DR OMA; YVSRPKF; -.
DR TreeFam; TF337039; -.
DR ChiTaRS; Gm960; mouse.
DR PRO; PR:J3QMY9; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; J3QMY9; protein.
DR Bgee; ENSMUSG00000071691; Expressed in white adipose tissue and 58 other tissues.
DR ExpressionAtlas; J3QMY9; baseline and differential.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; TAS:UniProtKB.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR InterPro; IPR028040; TopoVIB-like.
DR PANTHER; PTHR14652; PTHR14652; 1.
DR Pfam; PF15091; DUF4554; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Meiosis; Reference proteome.
FT CHAIN 1..579
FT /note="Type 2 DNA topoisomerase 6 subunit B-like"
FT /id="PRO_0000436277"
FT REGION 480..554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..530
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 159..162
FT /note="AENE -> SGRE (in isoform 2)"
FT /id="VSP_058336"
FT VAR_SEQ 163..579
FT /note="Missing (in isoform 2)"
FT /id="VSP_058337"
SQ SEQUENCE 579 AA; 63797 MW; 825591FDF32E0A8D CRC64;
MERTALAVCE ILRYLIIHWK CEAGTAKGTL LDGQLVISIE ALRSKHLPDS LHCIITIAST
RSVYGGLNFK KFLQEIQPAL PRLSAKLALA SEEGGRSQDA SGIAPCQVTF EVDENSQSLM
TDCLVIKHFL RKIIIVHHKL KFSFSVAVNG TLSAETFGAE NEPTLRLDNG VTLVVGFQRY
VSKPKLNWSE AHCSRIHPVL GHPAPLFIPD AKADTGLLGE LTLTPAAALC PSPKGFSSQL
CRISSVSIFL YGPLGLPLLS SDQDQPSTAV FRDTSYFIDW KKYNLFMVPN LDLNLDTQSV
LPDVNYKAES PEGNQSQNMN AQGPALLLFL FVDFQSDVPV QQAKIWGLHT LLTAHLSAIL
SESRSTVQQS IQSAVDQVWQ LYHHDAKTQQ RLQASLSVAV NSIMSVLTGS TRSSFRKTCL
QALEAADTQE FGVKLHRIFY DITQHQFLKH CSCDTEQHLT PEKNISAQNT KDQHKNIAQE
FPEESIGQAE NKRPKRGSPN HGREESRVLG SARDRSPPKS ATRDRELTEV SLTARGSQTQ
AAHGRAQAAE AASPAGGLED LWLQEVSNLS EWLNPGHRS
