TO6BL_SCHPO
ID TO6BL_SCHPO Reviewed; 260 AA.
AC P40385; Q9USW7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 08-JUN-2016, sequence version 3.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Meiotic recombination protein rec6 {ECO:0000303|PubMed:8005432};
GN Name=rec6 {ECO:0000303|PubMed:8005432};
GN ORFNames=SPBC21B10.12 {ECO:0000312|PomBase:SPBC21B10.12};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=8005432; DOI=10.1093/genetics/136.3.769;
RA Lin Y., Smith G.R.;
RT "Transient, meiosis-induced expression of the rec6 and rec12 genes of
RT Schizosaccharomyces pombe.";
RL Genetics 136:769-779(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION.
RX PubMed=10882124; DOI=10.1016/s1097-2765(00)80328-7;
RA Cervantes M.D., Farah J.A., Smith G.R.;
RT "Meiotic DNA breaks associated with recombination in S. pombe.";
RL Mol. Cell 5:883-888(2000).
RN [4]
RP IDENTIFICATION IN DSBC COMPLEX.
RX PubMed=22841486; DOI=10.1016/j.molcel.2012.06.023;
RA Miyoshi T., Ito M., Kugou K., Yamada S., Furuichi M., Oda A., Yamada T.,
RA Hirota K., Masai H., Ohta K.;
RT "A central coupler for recombination initiation linking chromosome
RT architecture to S phase checkpoint.";
RL Mol. Cell 47:722-733(2012).
RN [5]
RP REVISION OF GENE MODEL.
RX PubMed=26917764; DOI=10.1126/science.aad5309;
RA Robert T., Nore A., Brun C., Maffre C., Crimi B., Bourbon H.M.,
RA de Massy B.;
RT "The TopoVIB-Like protein family is required for meiotic DNA double-strand
RT break formation.";
RL Science 351:943-949(2016).
CC -!- FUNCTION: Required for formation of the rec12-mediated double-strand
CC breaks (DSBs) that initiate meiotic recombination (PubMed:10882124).
CC May be involved primarily in the early steps of meiotic recombination
CC (Probable). {ECO:0000269|PubMed:10882124, ECO:0000305|PubMed:8005432}.
CC -!- SUBUNIT: Component of the DSB catalytic core (DSBC) complex, composed
CC of at least rec12, rec6 and rec14. The complex interacts with mde2.
CC {ECO:0000269|PubMed:22841486}.
CC -!- DEVELOPMENTAL STAGE: Most abundant at 2 hours after induction of
CC meiosis, at the time of premeiotic DNA synthesis. Found at much lower
CC levels before and after this time. {ECO:0000269|PubMed:8005432}.
CC -!- MISCELLANEOUS: Despite weak sequence similarities, may correspond to
CC the subunit B of a rec12-containing topoisomerase 6 complex
CC specifically required for meiotic recombination. Retains some of the
CC structural features of the ancestral archaeal Top6B subunit (AC
CC O05207). {ECO:0000305|PubMed:26917764}.
CC -!- SIMILARITY: Belongs to the TOP6B-like family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA53084.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB57918.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; L14773; AAA53084.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CU329671; CAB57918.1; ALT_SEQ; Genomic_DNA.
DR PIR; T39915; T39915.
DR RefSeq; NP_595675.1; NM_001021570.2.
DR AlphaFoldDB; P40385; -.
DR BioGRID; 277114; 9.
DR STRING; 4896.SPBC21B10.12.1; -.
DR PaxDb; P40385; -.
DR EnsemblFungi; SPBC21B10.12.1; SPBC21B10.12.1:pep; SPBC21B10.12.
DR GeneID; 2540588; -.
DR KEGG; spo:SPBC21B10.12; -.
DR PomBase; SPBC21B10.12; rec6.
DR HOGENOM; CLU_1422175_0_0_1; -.
DR PRO; PR:P40385; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; NAS:PomBase.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:PomBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:PomBase.
PE 1: Evidence at protein level;
KW Meiosis; Reference proteome.
FT CHAIN 1..260
FT /note="Meiotic recombination protein rec6"
FT /id="PRO_0000097225"
FT REGION 197..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 260 AA; 29953 MW; 260B48875824CE87 CRC64;
MHRVRSARDG IVLKYHFKED WDETQNSPID CIASSRLKFW KRLNFNIYLD PEVSNQNLKL
LKTLTLHCDF QSPKKNLTMS KFKAMAVELI HEAFLKHIVS ILSKECPLSF QPSYYSKMFN
AMSNANIIAG SISRILTRES LDKSFEQSHF QLLMYKKCKL IYTRTLRKNE FDGIHKFPDL
EDSKQKLQVA DDEENKQYSE SSLLDDSQLL CSSPPVDSTE EARTCNVEED QDEILSVALV
TTDTILSSDN FVNDMLSAAD
