TO6BL_YEAST
ID TO6BL_YEAST Reviewed; 264 AA.
AC Q02721; D6VYX1;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Meiotic recombination protein REC102;
GN Name=REC102 {ECO:0000303|PubMed:2060778};
GN OrderedLocusNames=YLR329W {ECO:0000312|SGD:S000004321}; ORFNames=L8543.17;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1545806; DOI=10.1128/mcb.12.3.1248-1256.1992;
RA Cool M., Malone R.E.;
RT "Molecular and genetic analysis of the yeast early meiotic recombination
RT genes REC102 and REC107/MER2.";
RL Mol. Cell. Biol. 12:1248-1256(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 65-264.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=2060778; DOI=10.1093/genetics/128.1.79;
RA Malone R.E., Bullard S., Hermiston M., Rieger R., Cool M., Galbraith A.;
RT "Isolation of mutants defective in early steps of meiotic recombination in
RT the yeast Saccharomyces cerevisiae.";
RL Genetics 128:79-88(1991).
RN [6]
RP FUNCTION.
RX PubMed=1732169; DOI=10.1093/genetics/130.1.59;
RA Bhargava J., Engebrecht J., Roeder G.S.;
RT "The rec102 mutant of yeast is defective in meiotic recombination and
RT chromosome synapsis.";
RL Genetics 130:59-69(1992).
RN [7]
RP FUNCTION.
RX PubMed=10224247; DOI=10.1093/genetics/152.1.117;
RA Jiao K., Bullard S.A., Salem L., Malone R.E.;
RT "Coordination of the initiation of recombination and the reductional
RT division in meiosis in Saccharomyces cerevisiae.";
RL Genetics 152:117-128(1999).
RN [8]
RP FUNCTION, INTERACTION WITH SPO11, AND SUBCELLULAR LOCATION.
RX PubMed=11805049; DOI=10.1093/genetics/160.1.111;
RA Kee K., Keeney S.;
RT "Functional interactions between SPO11 and REC102 during initiation of
RT meiotic recombination in Saccharomyces cerevisiae.";
RL Genetics 160:111-122(2002).
RN [9]
RP INTERACTION WITH REC104 AND SPO11.
RX PubMed=12897161; DOI=10.1128/mcb.23.16.5928-5938.2003;
RA Jiao K., Salem L., Malone R.E.;
RT "Support for a meiotic recombination initiation complex: interactions among
RT Rec102p, Rec104p, and Spo11p.";
RL Mol. Cell. Biol. 23:5928-5938(2003).
RN [10]
RP INTERACTION WITH REC104, AND SUBCELLULAR LOCATION.
RX PubMed=15044957; DOI=10.1038/sj.emboj.7600184;
RA Kee K., Protacio R.U., Arora C., Keeney S.;
RT "Spatial organization and dynamics of the association of Rec102 and Rec104
RT with meiotic chromosomes.";
RL EMBO J. 23:1815-1824(2004).
RN [11]
RP FUNCTION.
RX PubMed=15655113; DOI=10.1101/gad.321105;
RA Prieler S., Penkner A., Borde V., Klein F.;
RT "The control of Spo11's interaction with meiotic recombination hotspots.";
RL Genes Dev. 19:255-269(2005).
RN [12]
RP IDENTIFICATION OF INTRON, AND INTERACTION WITH MEI4; REC104; REC114 AND
RP SPO11.
RX PubMed=17558514; DOI=10.1007/s00412-007-0111-y;
RA Maleki S., Neale M.J., Arora C., Henderson K.A., Keeney S.;
RT "Interactions between Mei4, Rec114, and other proteins required for meiotic
RT DNA double-strand break formation in Saccharomyces cerevisiae.";
RL Chromosoma 116:471-486(2007).
RN [13]
RP FUNCTION.
RX PubMed=17264124; DOI=10.1093/nar/gkl1162;
RA Sasanuma H., Murakami H., Fukuda T., Shibata T., Nicolas A., Ohta K.;
RT "Meiotic association between Spo11 regulated by Rec102, Rec104 and
RT Rec114.";
RL Nucleic Acids Res. 35:1119-1133(2007).
RN [14]
RP DEVELOPMENTAL STAGE.
RX PubMed=17244705; DOI=10.1073/pnas.0610354104;
RA Juneau K., Palm C., Miranda M., Davis R.W.;
RT "High-density yeast-tiling array reveals previously undiscovered introns
RT and extensive regulation of meiotic splicing.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007).
RN [15]
RP IDENTIFICATION.
RX PubMed=26917764; DOI=10.1126/science.aad5309;
RA Robert T., Nore A., Brun C., Maffre C., Crimi B., Bourbon H.M.,
RA de Massy B.;
RT "The TopoVIB-Like protein family is required for meiotic DNA double-strand
RT break formation.";
RL Science 351:943-949(2016).
CC -!- FUNCTION: Required for formation of the SPO11-mediated double-strand
CC breaks (DSBs) that initiate meiotic recombination. May mediate the
CC interaction between SPO11 subunits during meiosis. Also needed for
CC homolog chromosome pairing, synaptonemal complex formation, and for the
CC proper timing of the first meiotic division. Not required for mitosis
CC and mitotic DNA repair mechanisms. {ECO:0000269|PubMed:10224247,
CC ECO:0000269|PubMed:11805049, ECO:0000269|PubMed:15655113,
CC ECO:0000269|PubMed:17264124, ECO:0000269|PubMed:1732169}.
CC -!- SUBUNIT: Interacts with REC104; seems to form a functional unit with
CC REC104. REC102-REC104 interacts with SKI8-SPO11 and this interaction is
CC required for proper subcellular location of the proteins during the
CC initiation of recombination. Interacts with MEI4, REC114 and SPO11.
CC {ECO:0000269|PubMed:11805049, ECO:0000269|PubMed:12897161,
CC ECO:0000269|PubMed:15044957, ECO:0000269|PubMed:17558514}.
CC -!- INTERACTION:
CC Q02721; P33323: REC104; NbExp=6; IntAct=EBI-14432, EBI-16412992;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11805049,
CC ECO:0000269|PubMed:15044957}. Note=Associates with chromatin during
CC prophase. Recruitment to chromatin depends on REC104, SPO11 and SKI8.
CC -!- DEVELOPMENTAL STAGE: Meiosis-specific. mRNA is spliced only during
CC meiosis. {ECO:0000269|PubMed:17244705}.
CC -!- DISRUPTION PHENOTYPE: Reduces meiotic recombination several hundred
CC fold. Specifically affects meiosis, and does not have any detectable
CC mitotic phenotype. {ECO:0000269|PubMed:2060778}.
CC -!- MISCELLANEOUS: Despite weak sequence similarities, may correspond to
CC the subunit B of a SPO11-containing topoisomerase 6 complex
CC specifically required for meiotic recombination. Retains some of the
CC structural features of the ancestral archaeal Top6B subunit (AC
CC O05207). {ECO:0000305|PubMed:26917764}.
CC -!- SIMILARITY: Belongs to the TOP6B-like family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA34964.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAB64525.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; M74045; AAA34964.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U20618; AAB64525.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY558220; AAS56546.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09637.1; -; Genomic_DNA.
DR PIR; S20118; S20118.
DR RefSeq; NP_013433.2; NM_001182218.1.
DR AlphaFoldDB; Q02721; -.
DR BioGRID; 31593; 76.
DR ComplexPortal; CPX-1292; REC102-REC104 meiotic recombination initiation complex.
DR DIP; DIP-4675N; -.
DR IntAct; Q02721; 7.
DR STRING; 4932.YLR329W; -.
DR PaxDb; Q02721; -.
DR PRIDE; Q02721; -.
DR EnsemblFungi; YLR329W_mRNA; YLR329W; YLR329W.
DR GeneID; 851040; -.
DR KEGG; sce:YLR329W; -.
DR SGD; S000004321; REC102.
DR VEuPathDB; FungiDB:YLR329W; -.
DR eggNOG; ENOG502S4ZG; Eukaryota.
DR HOGENOM; CLU_094693_0_0_1; -.
DR InParanoid; Q02721; -.
DR OMA; HTHAKGY; -.
DR BioCyc; YEAST:G3O-32411-MON; -.
DR PRO; PR:Q02721; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q02721; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IDA:ComplexPortal.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
PE 1: Evidence at protein level;
KW Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..264
FT /note="Meiotic recombination protein REC102"
FT /id="PRO_0000097213"
SQ SEQUENCE 264 AA; 30237 MW; 0C332BFD12881126 CRC64;
MARDITFLTV FLESCGAVNN DEAGKLLSAW TSTVRIEGPE STDSNSLYIP LLPPGMLKIK
LNFKMNDRLV TEEQELFTKL REIVGSSIRF WEEQLFYQVQ DVSTIENHVI LSLKCTILTD
AQISTFISKP RELHTHAKGY PEIYYLSELS TTVNFFSKEG NYVEISQVIP HFNEYFSSLI
VSQLEFEYPM VFSMISRLRL KWQQSSLAPI SYALTSNSVL LPIMLNMIAQ DKSSTTAYQI
LCRRRGPPIQ NFQIFSLPAV TYNK
