TOA1_YEAST
ID TOA1_YEAST Reviewed; 286 AA.
AC P32773; D6W2Q3; E9P936;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Transcription initiation factor IIA large subunit;
DE Short=TFIIA large subunit;
DE AltName: Full=TFIIA 32 kDa subunit;
GN Name=TOA1; OrderedLocusNames=YOR194C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 9-21 AND 23-39.
RX PubMed=1546313; DOI=10.1126/science.1546313;
RA Ranish J.A., Lane W.S., Hahn S.;
RT "Isolation of two genes that encode subunits of the yeast transcription
RT factor IIA.";
RL Science 255:1127-1129(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP INTERACTION WITH KAP122 AND TOA2, AND SUBCELLULAR LOCATION.
RX PubMed=10525531; DOI=10.1083/jcb.147.2.235;
RA Titov A.A., Blobel G.;
RT "The karyopherin Kap122p/Pdr6p imports both subunits of the transcription
RT factor IIA into the nucleus.";
RL J. Cell Biol. 147:235-246(1999).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 2-54 AND 210-286.
RX PubMed=8610010; DOI=10.1038/381127a0;
RA Tan S., Hunziker Y., Sargent D.F., Richmond T.J.;
RT "Crystal structure of a yeast TFIIA/TBP/DNA complex.";
RL Nature 381:127-134(1996).
CC -!- FUNCTION: TFIIA is a component of the transcription machinery of RNA
CC polymerase II and implicated in the regulation of basal transcription.
CC Interacts with TBP (the TATA-binding protein).
CC -!- SUBUNIT: TFIIA is a heterodimer composed of the large TOA1 and a small
CC TOA2 subunits. Interacts with KAP122. {ECO:0000269|PubMed:10525531}.
CC -!- INTERACTION:
CC P32773; P32774: TOA2; NbExp=2; IntAct=EBI-19316, EBI-19323;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10525531}. Nucleus
CC {ECO:0000269|PubMed:10525531}. Note=Imported to nucleus via interaction
CC with KAP122.
CC -!- SIMILARITY: Belongs to the TFIIA subunit 1 family. {ECO:0000305}.
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DR EMBL; M85248; AAA19654.1; -; Unassigned_DNA.
DR EMBL; Z75102; CAA99407.1; -; Genomic_DNA.
DR EMBL; AY693223; AAT93242.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10969.1; -; Genomic_DNA.
DR PIR; B41810; B41810.
DR RefSeq; NP_014837.1; NM_001183613.1.
DR PDB; 1NH2; X-ray; 1.90 A; B=2-54, C=210-286.
DR PDB; 1RM1; X-ray; 2.50 A; C=1-286.
DR PDB; 1YTF; X-ray; 2.50 A; B=2-54, C=210-286.
DR PDB; 5FMF; EM; 6.00 A; M=2-286.
DR PDB; 5FYW; EM; 4.35 A; U=1-286.
DR PDB; 5FZ5; EM; 8.80 A; U=1-286.
DR PDB; 5OQJ; EM; 4.70 A; U=1-286.
DR PDB; 5OQM; EM; 5.80 A; U=1-286.
DR PDB; 5SVA; EM; 15.30 A; d=1-286.
DR PDB; 6GYK; EM; 5.10 A; U=1-286.
DR PDB; 6GYL; EM; 4.80 A; U=1-286.
DR PDB; 6GYM; EM; 6.70 A; U=1-286.
DR PDB; 7O4I; EM; 3.20 A; U=1-286.
DR PDB; 7O4J; EM; 2.90 A; U=1-286.
DR PDB; 7O72; EM; 3.40 A; U=1-286.
DR PDB; 7O73; EM; 3.40 A; U=1-286.
DR PDB; 7O75; EM; 3.20 A; U=1-286.
DR PDB; 7OH9; EM; 3.00 A; L=1-286.
DR PDB; 7OHA; EM; 2.90 A; L=1-286.
DR PDBsum; 1NH2; -.
DR PDBsum; 1RM1; -.
DR PDBsum; 1YTF; -.
DR PDBsum; 5FMF; -.
DR PDBsum; 5FYW; -.
DR PDBsum; 5FZ5; -.
DR PDBsum; 5OQJ; -.
DR PDBsum; 5OQM; -.
DR PDBsum; 5SVA; -.
DR PDBsum; 6GYK; -.
DR PDBsum; 6GYL; -.
DR PDBsum; 6GYM; -.
DR PDBsum; 7O4I; -.
DR PDBsum; 7O4J; -.
DR PDBsum; 7O72; -.
DR PDBsum; 7O73; -.
DR PDBsum; 7O75; -.
DR PDBsum; 7OH9; -.
DR PDBsum; 7OHA; -.
DR AlphaFoldDB; P32773; -.
DR SMR; P32773; -.
DR BioGRID; 34592; 276.
DR ComplexPortal; CPX-1633; Transcription factor TFIIA complex.
DR DIP; DIP-2347N; -.
DR IntAct; P32773; 2.
DR MINT; P32773; -.
DR STRING; 4932.YOR194C; -.
DR iPTMnet; P32773; -.
DR MaxQB; P32773; -.
DR PaxDb; P32773; -.
DR PRIDE; P32773; -.
DR TopDownProteomics; P32773; -.
DR EnsemblFungi; YOR194C_mRNA; YOR194C; YOR194C.
DR GeneID; 854369; -.
DR KEGG; sce:YOR194C; -.
DR SGD; S000005720; TOA1.
DR VEuPathDB; FungiDB:YOR194C; -.
DR eggNOG; KOG2652; Eukaryota.
DR GeneTree; ENSGT00940000169791; -.
DR HOGENOM; CLU_030027_2_1_1; -.
DR InParanoid; P32773; -.
DR OMA; ARWKCSL; -.
DR BioCyc; YEAST:G3O-33703-MON; -.
DR Reactome; R-SCE-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SCE-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SCE-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SCE-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SCE-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SCE-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SCE-9018519; Estrogen-dependent gene expression.
DR EvolutionaryTrace; P32773; -.
DR PRO; PR:P32773; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; P32773; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:ComplexPortal.
DR GO; GO:0005672; C:transcription factor TFIIA complex; IDA:SGD.
DR GO; GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IMP:CAFA.
DR GO; GO:0017025; F:TBP-class protein binding; IPI:CAFA.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IDA:ComplexPortal.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IDA:SGD.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:SGD.
DR DisProt; DP00104; -.
DR Gene3D; 2.30.18.10; -; 1.
DR InterPro; IPR004855; TFIIA_asu/bsu.
DR InterPro; IPR009088; TFIIA_b-brl.
DR PANTHER; PTHR12694; PTHR12694; 1.
DR Pfam; PF03153; TFIIA; 2.
DR SMART; SM01371; TFIIA; 1.
DR SUPFAM; SSF50784; SSF50784; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; Nucleus;
KW Reference proteome; Transcription.
FT CHAIN 1..286
FT /note="Transcription initiation factor IIA large subunit"
FT /id="PRO_0000072617"
FT REGION 120..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..236
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 15
FT /note="E -> Q (in Ref. 4; AAT93242)"
FT /evidence="ECO:0000305"
FT HELIX 4..20
FT /evidence="ECO:0007829|PDB:1NH2"
FT HELIX 22..27
FT /evidence="ECO:0007829|PDB:1NH2"
FT HELIX 32..47
FT /evidence="ECO:0007829|PDB:1NH2"
FT HELIX 57..60
FT /evidence="ECO:0007829|PDB:1RM1"
FT STRAND 243..254
FT /evidence="ECO:0007829|PDB:1NH2"
FT STRAND 257..269
FT /evidence="ECO:0007829|PDB:1NH2"
FT STRAND 272..284
FT /evidence="ECO:0007829|PDB:1NH2"
SQ SEQUENCE 286 AA; 32201 MW; 5BF0C39581866C98 CRC64;
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF
NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL PNINSNNNIP HSGETNINTN
TVEATNNSGA TLNTNTSGNT NADVTSQPKI EVKPEIELTI NNANITTVEN IDDESEKKDD
EEKEEDVEKT RKEKEQIEQV KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD
ENLMLCLYDK VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV
