TOB1_MOUSE
ID TOB1_MOUSE Reviewed; 362 AA.
AC Q61471; Q640M3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Protein Tob1;
DE AltName: Full=Transducer of erbB-2 1;
GN Name=Tob1; Synonyms=Tob, Trob;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ; TISSUE=Liver;
RX PubMed=9210596; DOI=10.1016/s0378-1119(97)00049-8;
RA Yoshida Y., Matuda S., Yamamoto T.;
RT "Cloning and characterization of the mouse tob gene.";
RL Gene 191:109-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, NUCLEAR EXPORT SIGNAL,
RP AND MUTAGENESIS OF 22-ARG--ARG-24; 37-LYS--LYS-39; LEU-234 AND LEU-236.
RX PubMed=15235587; DOI=10.1038/sj.onc.1207890;
RA Kawamura-Tsuzuku J., Suzuki T., Yoshida Y., Yamamoto T.;
RT "Nuclear localization of Tob is important for regulation of its
RT antiproliferative activity.";
RL Oncogene 23:6630-6638(2004).
CC -!- FUNCTION: Anti-proliferative protein; the function is mediated by
CC association with deadenylase subunits of the CCR4-NOT complex. Mediates
CC CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting
CC CNOT7 which leads to target mRNA deadenylation and decay.
CC {ECO:0000250|UniProtKB:P50616}.
CC -!- SUBUNIT: Interacts with ERBB2. Interacts with CNOT7. Interacts with
CC CPEB3 (via C-terminal RNA-binding region); recruits CNOT7 to CPEB3 to
CC form a ternary complex required for mRNA deadenylation and decay.
CC Interacts with CNOT8. Interacts with CPEB4.
CC {ECO:0000250|UniProtKB:P50616, ECO:0000250|UniProtKB:Q8R5K6}.
CC -!- INTERACTION:
CC Q61471; P57737: CORO7; Xeno; NbExp=3; IntAct=EBI-8527498, EBI-6916167;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15235587}. Nucleus
CC {ECO:0000269|PubMed:15235587}. Note=Only a small fraction localizes to
CC the cytoplasm except in late S-phase where more than half of proteins
CC become cytoplasmic.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: Phosphorylated on Ser and Thr residues. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BTG family. {ECO:0000305}.
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DR EMBL; D78382; BAA11384.1; -; Genomic_DNA.
DR EMBL; AL645846; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC082588; AAH82588.1; -; mRNA.
DR CCDS; CCDS25250.1; -.
DR RefSeq; NP_033453.2; NM_009427.2.
DR AlphaFoldDB; Q61471; -.
DR SMR; Q61471; -.
DR BioGRID; 204322; 2.
DR IntAct; Q61471; 1.
DR MINT; Q61471; -.
DR STRING; 10090.ENSMUSP00000036039; -.
DR iPTMnet; Q61471; -.
DR PhosphoSitePlus; Q61471; -.
DR PaxDb; Q61471; -.
DR PRIDE; Q61471; -.
DR ProteomicsDB; 259485; -.
DR Antibodypedia; 4398; 295 antibodies from 32 providers.
DR DNASU; 22057; -.
DR Ensembl; ENSMUST00000041589; ENSMUSP00000036039; ENSMUSG00000037573.
DR GeneID; 22057; -.
DR KEGG; mmu:22057; -.
DR UCSC; uc007kyf.1; mouse.
DR CTD; 10140; -.
DR MGI; MGI:1349721; Tob1.
DR VEuPathDB; HostDB:ENSMUSG00000037573; -.
DR eggNOG; KOG4006; Eukaryota.
DR GeneTree; ENSGT00940000154208; -.
DR HOGENOM; CLU_034687_0_0_1; -.
DR InParanoid; Q61471; -.
DR OMA; MKNSGRG; -.
DR OrthoDB; 1439942at2759; -.
DR PhylomeDB; Q61471; -.
DR TreeFam; TF105274; -.
DR BioGRID-ORCS; 22057; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Tob1; mouse.
DR PRO; PR:Q61471; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q61471; protein.
DR Bgee; ENSMUSG00000037573; Expressed in extensor digitorum longus and 259 other tissues.
DR Genevisible; Q61471; MM.
DR GO; GO:0030014; C:CCR4-NOT complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0030971; F:receptor tyrosine kinase binding; ISS:UniProtKB.
DR GO; GO:0046332; F:SMAD binding; IDA:MGI.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:MGI.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IDA:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0060212; P:negative regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISS:UniProtKB.
DR GO; GO:0045668; P:negative regulation of osteoblast differentiation; IMP:MGI.
DR GO; GO:0017148; P:negative regulation of translation; ISS:UniProtKB.
DR GO; GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; ISS:UniProtKB.
DR GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISS:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0060390; P:regulation of SMAD protein signal transduction; IDA:MGI.
DR Gene3D; 3.90.640.90; -; 1.
DR InterPro; IPR002087; Anti_prolifrtn.
DR InterPro; IPR036054; BTG-like_sf.
DR InterPro; IPR015677; Tob1.
DR InterPro; IPR015676; Tob1/2.
DR PANTHER; PTHR17537; PTHR17537; 1.
DR PANTHER; PTHR17537:SF6; PTHR17537:SF6; 1.
DR Pfam; PF07742; BTG; 1.
DR PRINTS; PR00310; ANTIPRLFBTG1.
DR SMART; SM00099; btg1; 1.
DR SUPFAM; SSF160696; SSF160696; 1.
DR PROSITE; PS00960; BTG_1; 1.
DR PROSITE; PS01203; BTG_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..362
FT /note="Protein Tob1"
FT /id="PRO_0000143813"
FT REGION 82..92
FT /note="Important for nuclear localization"
FT REGION 144..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 161..220
FT /note="Required for interaction with CPEB3"
FT /evidence="ECO:0000250|UniProtKB:P50616"
FT REGION 234..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 22..39
FT /note="Bipartite nuclear localization signal"
FT MOTIF 228..236
FT /note="Nuclear export signal"
FT COMPBIAS 234..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 204
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P50616"
FT MUTAGEN 22..24
FT /note="RRR->QQQ: Localizes to cytoplasm; when associated
FT with 37-NNN-39."
FT /evidence="ECO:0000269|PubMed:15235587"
FT MUTAGEN 37..39
FT /note="KKK->NNN: Localizes to cytoplasm; when associated
FT with 22-QQQ-24."
FT /evidence="ECO:0000269|PubMed:15235587"
FT MUTAGEN 234
FT /note="L->A: Not exported to the cytoplasm; when associated
FT with A-236."
FT /evidence="ECO:0000269|PubMed:15235587"
FT MUTAGEN 236
FT /note="L->A: Not exported to the cytoplasm; when associated
FT with A-234."
FT /evidence="ECO:0000269|PubMed:15235587"
FT CONFLICT 136
FT /note="A -> P (in Ref. 1; BAA11384)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="D -> H (in Ref. 1; BAA11384)"
FT /evidence="ECO:0000305"
FT CONFLICT 271
FT /note="P -> PQ (in Ref. 1; BAA11384)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 362 AA; 40056 MW; 4D4F1F492A7CA6FC CRC64;
MQLEIQVALN FIISYLYNKL PRRRVNIFGE ELERLLKKKY EGHWYPEKPY KGSGFRCIHV
GEKVDPVIEQ ASKESGLDID DVRGNLPQDL SVWIDPFEVS YQIGEKGPVK VLYVDDSSET
GCELDKEIKN SFNPEAQVFM PISDPASSVS SSPSPPFGHS AAVSPTFMPR STQPLTFTTA
TFAATKFGST KMKNSGRSSK VARTSPINLG LTVNVNDLLK QKAISSSVHS LYGLGLGSQQ
QPQPQPQQQQ QQQPSSSQPP PPLPQQQQQQ PQQQQQQQQQ TSALSPNAKE FIFPNMQGQG
SSTNGMFPGD SPLNLSPLQY SNAFDVFAAY GGLNEKSFVD GLNFSLNNMQ YSNQQFQPVM
AN
