TOC75_ORYSJ
ID TOC75_ORYSJ Reviewed; 817 AA.
AC Q84Q83; Q10NF2;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Protein TOC75, chloroplastic;
DE AltName: Full=75 kDa translocon at the outer-envelope-membrane of chloroplasts;
DE Flags: Precursor;
GN Name=TOC75; OrderedLocusNames=Os03g0271200, LOC_Os03g16440;
GN ORFNames=OJ1261C08.8;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Mediates the insertion of proteins targeted to the outer
CC membrane of chloroplasts. Required for the import of protein precursors
CC into chloroplasts. Forms the voltage-dependent preprotein translocation
CC channels (hydrophilic beta barrel) of the TOC complex in the
CC chloroplastic outer membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the TOC core complex that includes a protein for the
CC specific recognition of transit peptides surrounded by a ring composed
CC of four proteins forming translocation channels, and four to five GTP-
CC binding proteins providing energy. This core complex can interact with
CC components of the TIC complex to form a larger import complex.
CC Chloroplastic protein precursors also interact with these complexes (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- DOMAIN: Transmembrane regions consist mainly of membrane-spanning sided
CC beta-sheets, which are not predicted by sequence analysis tools.
CC -!- SIMILARITY: Belongs to the TOC75 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP06869.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AK120241; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC135207; AAP06869.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF95211.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11603.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83476.1; -; Genomic_DNA.
DR EMBL; AK120241; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015630560.1; XM_015775074.1.
DR AlphaFoldDB; Q84Q83; -.
DR SMR; Q84Q83; -.
DR STRING; 4530.OS03T0271200-01; -.
DR PaxDb; Q84Q83; -.
DR PRIDE; Q84Q83; -.
DR EnsemblPlants; Os03t0271200-01; Os03t0271200-01; Os03g0271200.
DR GeneID; 4332389; -.
DR Gramene; Os03t0271200-01; Os03t0271200-01; Os03g0271200.
DR KEGG; osa:4332389; -.
DR eggNOG; ENOG502QTZ3; Eukaryota.
DR InParanoid; Q84Q83; -.
DR OMA; DERSHIC; -.
DR OrthoDB; 455003at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q84Q83; baseline and differential.
DR Genevisible; Q84Q83; OS.
DR GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0031359; C:integral component of chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0001401; C:SAM complex; IBA:GO_Central.
DR GO; GO:0009658; P:chloroplast organization; IBA:GO_Central.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IBA:GO_Central.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IBA:GO_Central.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR039910; D15-like.
DR PANTHER; PTHR12815; PTHR12815; 1.
DR Pfam; PF01103; Omp85; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Plastid outer membrane; Protein transport;
KW Reference proteome; Transit peptide; Transmembrane;
KW Transmembrane beta strand; Transport.
FT TRANSIT 1..31
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 32..135
FT /note="Chloroplast; outer membrane"
FT /evidence="ECO:0000250"
FT CHAIN 136..817
FT /note="Protein TOC75, chloroplastic"
FT /id="PRO_0000042822"
FT TOPO_DOM 136..147
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..156
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..173
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..232
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..241
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..261
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..364
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..372
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..417
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..425
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 426..434
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..443
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..449
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..459
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..471
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..480
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 481..507
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 508..516
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 517..561
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 562..569
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 570..577
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 578..585
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 586..692
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 693..701
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 702..713
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 714..722
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 723..784
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 785..791
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 792..805
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 806..813
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 814..817
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT REGION 14..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 817 AA; 87687 MW; 97D4076C0A9F35DD CRC64;
MALTSQSLFF SPLAAGPSRR VRGRGRSTSV SAAASASSHN SQPHGHPQQP LAVASSSSKS
ESKGSKTFAL ASAITAAASG AFLLASSGGG FGGGAGGPLG GGGGGWGAGG GGGGGGGGGG
GGFWSRIFSG GAAHADEKSS GDWDPHGLPA NINVPMTKLS GLKRYKISEL KFFDRAAGGG
GAFTGPEDSF FEMVTLQPGG VYTKSQLLKE LETLVSCGMF ERVDLEGKAK PDGTLGLTVS
FVESVWSAAK QFKCINVGLM SQSGQVDFDQ DMTEREKMDY LRKQERDYQQ RVRGAKPCIL
PDNVRGEVLG MMKKQEKVSA RLLQRIRDHV QKWYHNEGFV CAQVVNFGNL NTSEVVCEVV
EGDITKVEYQ FQDKLGNFVE GNTQIPIIDR ELPQQLRPGH IFNIGAGKQA LKNINSLALF
SNIEVNPRPD ETKEGGIVVE IKLKELEPKS AEVSTEWSIV PGREGRPTLA SIQPGGTVSF
EHRNIYGLNR SIVGSVTSSN LLNPQDDLSF KLEYVHPYLD GVDDRNKNRT FKTSCFNTRK
LSPVFVAGPN MDEAPPVWVD RVGFKANITE SFTRQSKFTY GLVVEEITTR DETNSICTHG
SRAMPSGGLS MDGPPTTLSG TGIDRMAFLQ ANITRDNTEF VNGAVIGDRC IFQLDQGLGI
GSKNPFFNRH QLTLTKFVNL NKQEKGAGKP LPAVLVLHGH YAGCVGDLPS YDAFTLGGPY
SVRGYGMGEL GASRNVLEVA SELRIPVRNT YVYGFVEHGT DLGSSKDVKG NPTEFFRRVG
HGSSYGLGVK LGLVRGEYIV DHNAGTGTVF FRFGERF
