TOD6_YEAST
ID TOD6_YEAST Reviewed; 525 AA.
AC P34219; D6VPU6;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Transcriptional regulatory protein TOD6;
DE AltName: Full=PAC-binding factor 1;
DE AltName: Full=Twin of DOT6;
GN Name=TOD6; Synonyms=PBF1; OrderedLocusNames=YBL054W;
GN ORFNames=YBL0509, YBL0513;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8154187; DOI=10.1002/yea.320091210;
RA Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.;
RT "Sequencing and functional analysis of a 32,560 bp segment on the left arm
RT of yeast chromosome II. Identification of 26 open reading frames, including
RT the KIP1 and SEC17 genes.";
RL Yeast 9:1355-1371(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=16544271; DOI=10.1002/yea.1353;
RA Wade C.H., Umbarger M.A., McAlear M.A.;
RT "The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains
RT over 200 genes.";
RL Yeast 23:293-306(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [9]
RP IDENTIFICATION IN THE RPD3(L) COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=19040720; DOI=10.1186/gb-2008-9-11-r167;
RA Shevchenko A., Roguev A., Schaft D., Buchanan L., Habermann B., Sakalar C.,
RA Thomas H., Krogan N.J., Shevchenko A., Stewart A.F.;
RT "Chromatin Central: towards the comparative proteome by accurate mapping of
RT the yeast proteomic environment.";
RL Genome Biol. 9:R167.1-R167.22(2008).
RN [10]
RP DNA-BINDING.
RX PubMed=19111667; DOI=10.1016/j.molcel.2008.11.020;
RA Badis G., Chan E.T., van Bakel H., Pena-Castillo L., Tillo D., Tsui K.,
RA Carlson C.D., Gossett A.J., Hasinoff M.J., Warren C.L., Gebbia M.,
RA Talukder S., Yang A., Mnaimneh S., Terterov D., Coburn D., Li Yeo A.,
RA Yeo Z.X., Clarke N.D., Lieb J.D., Ansari A.Z., Nislow C., Hughes T.R.;
RT "A library of yeast transcription factor motifs reveals a widespread
RT function for Rsc3 in targeting nucleosome exclusion at promoters.";
RL Mol. Cell 32:878-887(2008).
RN [11]
RP FUNCTION.
RX PubMed=19158363; DOI=10.1101/gr.090233.108;
RA Zhu C., Byers K.J., McCord R.P., Shi Z., Berger M.F., Newburger D.E.,
RA Saulrieta K., Smith Z., Shah M.V., Radhakrishnan M., Philippakis A.A.,
RA Hu Y., De Masi F., Pacek M., Rolfs A., Murthy T.V.S., Labaer J.,
RA Bulyk M.L.;
RT "High-resolution DNA-binding specificity analysis of yeast transcription
RT factors.";
RL Genome Res. 19:556-566(2009).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280; SER-333 AND SER-341, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Component of the RPD3 histone deacetylase complex RPD3C(L)
CC responsible for the deacetylation of lysine residues on the N-terminal
CC part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation
CC gives a tag for epigenetic repression and plays an important role in
CC transcriptional regulation, cell cycle progression and developmental
CC events. TOD6 binds to sequences containing the core CGATG, which
CC resembles the PAC (Polymerase A and C) motif.
CC {ECO:0000269|PubMed:16544271, ECO:0000269|PubMed:19158363}.
CC -!- SUBUNIT: Component of the RPD3C(L) complex composed of at least ASH1,
CC CTI6, DEP1, DOT6, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, TOD6;
CC UME1 and UME6. {ECO:0000269|PubMed:19040720}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00625, ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 830 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DOT6 family. {ECO:0000305}.
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DR EMBL; Z23261; CAA80793.1; -; Genomic_DNA.
DR EMBL; Z35815; CAA84874.1; -; Genomic_DNA.
DR EMBL; AY692625; AAT92644.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07066.1; -; Genomic_DNA.
DR PIR; S39834; S39834.
DR RefSeq; NP_009499.1; NM_001178294.1.
DR AlphaFoldDB; P34219; -.
DR SMR; P34219; -.
DR BioGRID; 32645; 77.
DR DIP; DIP-2636N; -.
DR IntAct; P34219; 3.
DR MINT; P34219; -.
DR STRING; 4932.YBL054W; -.
DR iPTMnet; P34219; -.
DR MaxQB; P34219; -.
DR PaxDb; P34219; -.
DR PRIDE; P34219; -.
DR TopDownProteomics; P34219; -.
DR EnsemblFungi; YBL054W_mRNA; YBL054W; YBL054W.
DR GeneID; 852226; -.
DR KEGG; sce:YBL054W; -.
DR SGD; S000000150; TOD6.
DR VEuPathDB; FungiDB:YBL054W; -.
DR eggNOG; ENOG502RXV1; Eukaryota.
DR GeneTree; ENSGT00940000176456; -.
DR HOGENOM; CLU_018984_1_0_1; -.
DR InParanoid; P34219; -.
DR OMA; GWKEIAH; -.
DR BioCyc; YEAST:G3O-28952-MON; -.
DR PRO; PR:P34219; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P34219; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0070210; C:Rpd3L-Expanded complex; HDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:SGD.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..525
FT /note="Transcriptional regulatory protein TOD6"
FT /id="PRO_0000197146"
FT DOMAIN 67..124
FT /note="HTH myb-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 97..120
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 22..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 451..510
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 471..486
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 495..509
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 280
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 333
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 341
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 366
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
SQ SEQUENCE 525 AA; 59226 MW; 8663DFE2641AA72E CRC64;
MTLPKLSSVS VSSGHVSANS HGFSILSKHP HPNNLVHSHS LSHTNAKSHL PISSTSTKEN
STNKEEAESL KKNNPSSWDP SDDIKLRHLK EIKNLGWKEI AHHFPNRTPN ACQFRWRRLK
SGNLKSNKTA VIDINKLFGV YATGDATPSA GTPSAEEAVK EEAVEDEDIT AGSSAIEDSP
PDFKPLVKPK YMDRKLITQR STSTFSDHEP QHTKPRKLFV KPRSFSHSIT TNTPNVKTAQ
QTNLSLYNTT SAKTNKAVNS NDYENIGLVP KIIIRSRRNS FIPSTQIPHS TTKTRKNSHS
VISSRRSSFN MMHSRRSSFN SHAPTEPISR RASLVVSPYM SPRRLSTSQS VHYHPQHQYY
LNPIASPNCK TDHANDKITH TRTFLDMQKF ANKHPWSRED DEVLLNNTKD KQNHLSPLEI
SIVLPNNRSE LEIQQRMDYL KRKGRVSGFH TNEGCKDEEE EDDIDPLHKE NGINTPSQQS
QNYGMLEAKH DNPKSSELSS MTSANDIRNE QDELPGINSI FKNIF
