TODB_PSEP1
ID TODB_PSEP1 Reviewed; 107 AA.
AC A5W4F0; P13370;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Toluene 1,2-dioxygenase system ferredoxin subunit;
GN Name=todB; OrderedLocusNames=Pput_2879;
OS Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=351746;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-13.
RX PubMed=2670929; DOI=10.1016/s0021-9258(18)63793-7;
RA Zylstra G.J., Gibson D.T.;
RT "Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the
RT todC1C2BADE genes and their expression in Escherichia coli.";
RL J. Biol. Chem. 264:14940-14946(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT "Complete sequence of Pseudomonas putida F1.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This protein seems to be a 2Fe-2S ferredoxin.
CC -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC -!- SUBUNIT: This dioxygenase system consists of four proteins: the two
CC subunits of the hydroxylase component (todC1 and todC2), a ferredoxin
CC (TodB) and a ferredoxin reductase (TodA).
CC -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC ferredoxin component family. {ECO:0000305}.
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DR EMBL; J04996; AAA26007.1; -; Genomic_DNA.
DR EMBL; CP000712; ABQ79010.1; -; Genomic_DNA.
DR PIR; C36516; C36516.
DR RefSeq; WP_012052599.1; NC_009512.1.
DR PDB; 3DQY; X-ray; 1.20 A; A=2-107.
DR PDB; 4EMJ; X-ray; 2.40 A; B=1-107.
DR PDBsum; 3DQY; -.
DR PDBsum; 4EMJ; -.
DR AlphaFoldDB; A5W4F0; -.
DR SMR; A5W4F0; -.
DR STRING; 351746.Pput_2879; -.
DR EnsemblBacteria; ABQ79010; ABQ79010; Pput_2879.
DR KEGG; ppf:Pput_2879; -.
DR eggNOG; COG2146; Bacteria.
DR HOGENOM; CLU_055690_5_2_6; -.
DR OMA; TLECWLH; -.
DR OrthoDB; 1681886at2; -.
DR BioCyc; MetaCyc:MON-11351; -.
DR UniPathway; UPA00273; -.
DR EvolutionaryTrace; A5W4F0; -.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.102.10.10; -; 1.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Aromatic hydrocarbons catabolism;
KW Direct protein sequencing; Electron transport; Iron; Iron-sulfur;
KW Metal-binding; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2670929"
FT CHAIN 2..107
FT /note="Toluene 1,2-dioxygenase system ferredoxin subunit"
FT /id="PRO_0000314467"
FT DOMAIN 4..99
FT /note="Rieske"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 43
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 45
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 62
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 65
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:3DQY"
FT HELIX 9..11
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 23..25
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 27..32
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 35..42
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:3DQY"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 53..56
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:3DQY"
FT TURN 63..65
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:3DQY"
FT TURN 71..73
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 76..78
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:3DQY"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:3DQY"
FT HELIX 101..103
FT /evidence="ECO:0007829|PDB:3DQY"
SQ SEQUENCE 107 AA; 11890 MW; FDBFC82AD5C9C75C CRC64;
MTWTYILRQG DLPPGEMQRY EGGPEPVMVC NVDGEFFAVQ DTCTHGDWAL SDGYLDGDIV
ECTLHFGKFC VRTGKVKALP ACKPIKVFPI KVEGDEVHVD LDNGELK
