ID   TODF_PSEP1              Reviewed;         276 AA.
AC   P23133; A5W4F3;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 2.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=2-hydroxy-6-oxo-2,4-heptadienoate hydrolase;
DE            Short=HOHH;
DE            EC=3.7.1.25 {ECO:0000305|PubMed:7944378};
GN   Name=todF; OrderedLocusNames=Pput_2882;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=1916282; DOI=10.1016/0378-1119(91)90470-v;
RA   Menn F.M., Zylstra G.J., Gibson D.T.;
RT   "Location and sequence of the todF gene encoding 2-hydroxy-6-oxohepta-2,4-
RT   dienoate hydrolase in Pseudomonas putida F1.";
RL   Gene 104:91-94(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-276.
RX   PubMed=2670929; DOI=10.1016/s0021-9258(18)63793-7;
RA   Zylstra G.J., Gibson D.T.;
RT   "Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the
RT   todC1C2BADE genes and their expression in Escherichia coli.";
RL   J. Biol. Chem. 264:14940-14946(1989).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=7944378; DOI=10.1128/aem.60.9.3466-3469.1994;
RA   Haigler B.E., Wallace W.H., Spain J.C.;
RT   "Biodegradation of 2-nitrotoluene by Pseudomonas sp. strain JS42.";
RL   Appl. Environ. Microbiol. 60:3466-3469(1994).
CC   -!- FUNCTION: Catalyzes the hydrolysis of 2-hydroxy-6-oxohepta-2,4-dienoate
CC       into 2-hydroxypenta-2,4-dienoate and acetate.
CC       {ECO:0000269|PubMed:1916282, ECO:0000269|PubMed:7944378}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O = (2Z)-2-
CC         hydroxypenta-2,4-dienoate + acetate + H(+); Xref=Rhea:RHEA:59220,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:144490; EC=3.7.1.25;
CC         Evidence={ECO:0000305|PubMed:7944378};
CC   -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC   -!- SIMILARITY: Belongs to the DmpD/TodF/XylF esterase family.
CC       {ECO:0000305}.
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DR   EMBL; M64080; AAA25817.1; -; Genomic_DNA.
DR   EMBL; CP000712; ABQ79013.1; -; Genomic_DNA.
DR   EMBL; J04996; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; JH0245; JH0245.
DR   RefSeq; WP_012052602.1; NC_009512.1.
DR   AlphaFoldDB; P23133; -.
DR   SMR; P23133; -.
DR   STRING; 351746.Pput_2882; -.
DR   ESTHER; psepu-todf; Carbon-carbon_bond_hydrolase.
DR   PRIDE; P23133; -.
DR   EnsemblBacteria; ABQ79013; ABQ79013; Pput_2882.
DR   KEGG; ppf:Pput_2882; -.
DR   eggNOG; COG2267; Bacteria.
DR   HOGENOM; CLU_020336_13_2_6; -.
DR   OMA; HVPQIEC; -.
DR   OrthoDB; 1119700at2; -.
DR   BioCyc; MetaCyc:MON-11393; -.
DR   SABIO-RK; P23133; -.
DR   UniPathway; UPA00273; -.
DR   GO; GO:0018765; F:2-hydroxy-6-oxohepta-2,4-dienoate hydrolase activity; IEA:RHEA.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Hydrolase; Serine esterase.
FT   CHAIN           1..276
FT                   /note="2-hydroxy-6-oxo-2,4-heptadienoate hydrolase"
FT                   /id="PRO_0000207057"
FT   DOMAIN          28..259
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        105
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        226
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        254
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   276 AA;  30788 MW;  45EEEB2BAAA59107 CRC64;
     MTNVNAEIGR MVLAGGIETN LHDVGAGNPV VLVHGSGPGV TAWANWRTVM PELSRHRRVI
     APDMVGFGFT QRPHGIHYGV ESWVAHLAGI LDALELDRVD LVGNSFGGAL SLAFAIRFPH
     RVRRLVLMGA VGVSFELTDG LDAVWGYEPS VPNMRKVMDY FAYDRSLVSD ELAELRYKAS
     TRPGFQEAFA SMFPAPRQRW VDALASSDQD IRDIRHETLI LHGRDDRVIP LETSLRLNQL
     IEPSQLHVFG RCGHWVQIEQ NRGFIRLVND FLAAED