TODT_PSEP1
ID TODT_PSEP1 Reviewed; 227 AA.
AC A5W4E2; O07832;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Response regulator protein TodT;
GN Name=todT; OrderedLocusNames=Pput_2871;
OS Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=351746;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING, DISRUPTION
RP PHENOTYPE, GENE NAME, AND MUTAGENESIS OF ASP-77.
RC STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RX PubMed=9037074; DOI=10.1073/pnas.94.4.1453;
RA Lau P.C., Wang Y., Patel A., Labbe D., Bergeron H., Brousseau R.,
RA Konishi Y., Rawlings M.;
RT "A bacterial basic region leucine zipper histidine kinase regulating
RT toluene degradation.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:1453-1458(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT "Complete sequence of Pseudomonas putida F1.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Member of the two-component regulatory system TodS/TodT
CC involved in the regulation of toluene degradation. Phosphorylated TodT
CC activates transcription of the tod operon (todXFC1C2BADEGIH). Binds
CC specifically to a 6-bp palindromic DNA structure in the tod promoter
CC region. {ECO:0000269|PubMed:9037074}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Phosphorylated by TodS. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Mutants are unable to use toluene as the sole
CC carbon source, are incapable of converting indole to indigo and do not
CC have catechol dioxygenase activity. {ECO:0000269|PubMed:9037074}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABQ79002.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U72354; AAC45439.1; -; Genomic_DNA.
DR EMBL; CP000712; ABQ79002.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; A5W4E2; -.
DR SMR; A5W4E2; -.
DR STRING; 351746.Pput_2871; -.
DR EnsemblBacteria; ABQ79002; ABQ79002; Pput_2871.
DR KEGG; ppf:Pput_2871; -.
DR eggNOG; COG4566; Bacteria.
DR HOGENOM; CLU_000445_90_4_6; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..227
FT /note="Response regulator protein TodT"
FT /id="PRO_0000423594"
FT DOMAIN 28..142
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 158..223
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 182..201
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 77
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000305"
FT MUTAGEN 77
FT /note="D->N: Lacks regulatory activity but can still bind
FT DNA."
FT /evidence="ECO:0000269|PubMed:9037074"
SQ SEQUENCE 227 AA; 25432 MW; 7A5D506B9213E26D CRC64;
MPARWGCLFP GKYPCQTGLR HMSDRASVIY ILDDDNAVLE ALSSLVRSIG LSVECFSSAS
VFLNDVNRSA CGCLILDVRM PEMSGLDVQR QLKELGEQIP IIFISGHGDI PMAVKAIKAG
AVDFFTKPFR EEELLGAIRA ALKLAPQQRS NAPRVSELKE NYESLSKREQ QVLKFVLRGY
LNKQTALELD ISEATVKVHR HNIMRKMKVS SIQDLVRVTE RLKDSLE