ID   TODT_PSEP1              Reviewed;         227 AA.
AC   A5W4E2; O07832;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 2.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Response regulator protein TodT;
GN   Name=todT; OrderedLocusNames=Pput_2871;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING, DISRUPTION
RP   PHENOTYPE, GENE NAME, AND MUTAGENESIS OF ASP-77.
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RX   PubMed=9037074; DOI=10.1073/pnas.94.4.1453;
RA   Lau P.C., Wang Y., Patel A., Labbe D., Bergeron H., Brousseau R.,
RA   Konishi Y., Rawlings M.;
RT   "A bacterial basic region leucine zipper histidine kinase regulating
RT   toluene degradation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:1453-1458(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Member of the two-component regulatory system TodS/TodT
CC       involved in the regulation of toluene degradation. Phosphorylated TodT
CC       activates transcription of the tod operon (todXFC1C2BADEGIH). Binds
CC       specifically to a 6-bp palindromic DNA structure in the tod promoter
CC       region. {ECO:0000269|PubMed:9037074}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Phosphorylated by TodS. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Mutants are unable to use toluene as the sole
CC       carbon source, are incapable of converting indole to indigo and do not
CC       have catechol dioxygenase activity. {ECO:0000269|PubMed:9037074}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABQ79002.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U72354; AAC45439.1; -; Genomic_DNA.
DR   EMBL; CP000712; ABQ79002.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A5W4E2; -.
DR   SMR; A5W4E2; -.
DR   STRING; 351746.Pput_2871; -.
DR   EnsemblBacteria; ABQ79002; ABQ79002; Pput_2871.
DR   KEGG; ppf:Pput_2871; -.
DR   eggNOG; COG4566; Bacteria.
DR   HOGENOM; CLU_000445_90_4_6; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..227
FT                   /note="Response regulator protein TodT"
FT                   /id="PRO_0000423594"
FT   DOMAIN          28..142
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          158..223
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        182..201
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   MOD_RES         77
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000305"
FT   MUTAGEN         77
FT                   /note="D->N: Lacks regulatory activity but can still bind
FT                   DNA."
FT                   /evidence="ECO:0000269|PubMed:9037074"
SQ   SEQUENCE   227 AA;  25432 MW;  7A5D506B9213E26D CRC64;
     MPARWGCLFP GKYPCQTGLR HMSDRASVIY ILDDDNAVLE ALSSLVRSIG LSVECFSSAS
     VFLNDVNRSA CGCLILDVRM PEMSGLDVQR QLKELGEQIP IIFISGHGDI PMAVKAIKAG
     AVDFFTKPFR EEELLGAIRA ALKLAPQQRS NAPRVSELKE NYESLSKREQ QVLKFVLRGY
     LNKQTALELD ISEATVKVHR HNIMRKMKVS SIQDLVRVTE RLKDSLE