TODT_PSEPT
ID TODT_PSEPT Reviewed; 227 AA.
AC I7CA98; O07832;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=Response regulator protein TodT;
GN Name=todT; Synonyms=tobT; OrderedLocusNames=T1E_4277;
OS Pseudomonas putida (strain DOT-T1E).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1196325;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DOT-T1E;
RX PubMed=10333523; DOI=10.1016/s0378-1119(99)00113-4;
RA Mosqueda G., Ramos-Gonzalez M.I., Ramos J.L.;
RT "Toluene metabolism by the solvent-tolerant Pseudomonas putida DOT-T1
RT strain, and its role in solvent impermeabilization.";
RL Gene 232:69-76(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DOT-T1E;
RX PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT DOT-T1E deduced from its annotated genome.";
RL Microb. Biotechnol. 6:598-611(2013).
RN [3]
RP FUNCTION, DNA-BINDING, ACTIVITY REGULATION, AND PHOSPHORYLATION.
RC STRAIN=DOT-T1E;
RX PubMed=16702539; DOI=10.1073/pnas.0602902103;
RA Lacal J., Busch A., Guazzaroni M.E., Krell T., Ramos J.L.;
RT "The TodS-TodT two-component regulatory system recognizes a wide range of
RT effectors and works with DNA-bending proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:8191-8196(2006).
RN [4]
RP FUNCTION, DNA-BINDING, AND SUBUNIT.
RC STRAIN=DOT-T1E;
RX PubMed=18166197; DOI=10.1016/j.jmb.2007.12.004;
RA Lacal J., Guazzaroni M.E., Busch A., Krell T., Ramos J.L.;
RT "Hierarchical binding of the TodT response regulator to its multiple
RT recognition sites at the tod pathway operon promoter.";
RL J. Mol. Biol. 376:325-337(2008).
RN [5]
RP FUNCTION, AND DNA-BINDING.
RC STRAIN=DOT-T1E;
RX PubMed=18950641; DOI=10.1016/j.jmb.2008.10.011;
RA Lacal J., Guazzaroni M.E., Gutierrez-del-Arroyo P., Busch A., Velez M.,
RA Krell T., Ramos J.L.;
RT "Two levels of cooperativeness in the binding of TodT to the tod operon
RT promoter.";
RL J. Mol. Biol. 384:1037-1047(2008).
RN [6]
RP PHOSPHORYLATION BY TODS, AND MUTAGENESIS OF ASP-77.
RC STRAIN=DOT-T1E;
RX PubMed=19240030; DOI=10.1074/jbc.m900521200;
RA Busch A., Guazzaroni M.E., Lacal J., Ramos J.L., Krell T.;
RT "The sensor kinase TodS operates by a multiple step phosphorelay mechanism
RT involving two autokinase domains.";
RL J. Biol. Chem. 284:10353-10360(2009).
RN [7]
RP INDUCTION.
RC STRAIN=DOT-T1E;
RX PubMed=20543072; DOI=10.1128/jb.00379-10;
RA Busch A., Lacal J., Silva-Jimenez H., Krell T., Ramos J.L.;
RT "Catabolite repression of the TodS/TodT two-component system and effector-
RT dependent transphosphorylation of TodT as the basis for toluene dioxygenase
RT catabolic pathway control.";
RL J. Bacteriol. 192:4246-4250(2010).
RN [8]
RP FUNCTION IN TOLUENE DEGRADATION.
RC STRAIN=DOT-T1E;
RX PubMed=22212183; DOI=10.1111/j.1751-7915.2011.00322.x;
RA Silva-Jimenez H., Garcia-Fontana C., Cadirci B.H., Ramos-Gonzalez M.I.,
RA Ramos J.L., Krell T.;
RT "Study of the TmoS/TmoT two-component system: towards the functional
RT characterization of the family of TodS/TodT like systems.";
RL Microb. Biotechnol. 5:489-500(2012).
CC -!- FUNCTION: Member of the two-component regulatory system TodS/TodT
CC involved in the regulation of toluene degradation. Phosphorylated TodT
CC activates transcription of the tod operon (todXFC1C2BADEGIH). Binds
CC specifically to three boxes in the tod promoter region in a cooperative
CC manner. Boxes-1 and -2 are pseudopalindromes and Box-3 is a half-
CC palindrome. {ECO:0000269|PubMed:16702539, ECO:0000269|PubMed:18166197,
CC ECO:0000269|PubMed:18950641, ECO:0000269|PubMed:22212183}.
CC -!- ACTIVITY REGULATION: Phosphorylation by TodS probably induces
CC conformational changes, which allow or alter the interaction with RNA
CC polymerase in a process assisted by the integration host factor (IHF).
CC {ECO:0000269|PubMed:16702539}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:18166197}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Constitutively expressed. Is under catabolite repression.
CC {ECO:0000269|PubMed:20543072}.
CC -!- PTM: Phosphorylated by TodS. {ECO:0000269|PubMed:16702539,
CC ECO:0000269|PubMed:19240030}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFO50106.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; GQ884177; ADI95407.1; -; Genomic_DNA.
DR EMBL; CP003734; AFO50106.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; I7CA98; -.
DR SMR; I7CA98; -.
DR DIP; DIP-61172N; -.
DR IntAct; I7CA98; 1.
DR EnsemblBacteria; AFO50106; AFO50106; T1E_4277.
DR KEGG; ppx:T1E_4277; -.
DR PATRIC; fig|1196325.3.peg.4234; -.
DR HOGENOM; CLU_000445_90_4_6; -.
DR Proteomes; UP000006503; Chromosome.
DR CollecTF; EXPREG_000005e0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..227
FT /note="Response regulator protein TodT"
FT /id="PRO_0000423593"
FT DOMAIN 28..142
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 158..223
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 182..201
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 77
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000305"
FT MUTAGEN 77
FT /note="D->A: Lack of phosphorylation."
FT /evidence="ECO:0000269|PubMed:19240030"
SQ SEQUENCE 227 AA; 25432 MW; 7A5D506B9213E26D CRC64;
MPARWGCLFP GKYPCQTGLR HMSDRASVIY ILDDDNAVLE ALSSLVRSIG LSVECFSSAS
VFLNDVNRSA CGCLILDVRM PEMSGLDVQR QLKELGEQIP IIFISGHGDI PMAVKAIKAG
AVDFFTKPFR EEELLGAIRA ALKLAPQQRS NAPRVSELKE NYESLSKREQ QVLKFVLRGY
LNKQTALELD ISEATVKVHR HNIMRKMKVS SIQDLVRVTE RLKDSLE
