ID   TOF1_ASHGO              Reviewed;        1265 AA.
AC   Q752A3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Topoisomerase 1-associated factor 1;
GN   Name=TOF1; OrderedLocusNames=AFR672C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 408 AND 410.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Forms a fork protection complex (FPC) with CSM3 and which is
CC       required for chromosome segregation during meiosis and DNA damage
CC       repair. FPC coordinates leading and lagging strand synthesis and moves
CC       with the replication fork. FPC stabilizes replication forks in a
CC       configuration that is recognized by replication checkpoint sensors (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC       TOF1 and CSM3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS54044.2; -; Genomic_DNA.
DR   RefSeq; NP_986220.2; NM_212356.2.
DR   AlphaFoldDB; Q752A3; -.
DR   SMR; Q752A3; -.
DR   STRING; 33169.AAS54044; -.
DR   EnsemblFungi; AAS54044; AAS54044; AGOS_AFR672C.
DR   GeneID; 4622509; -.
DR   KEGG; ago:AGOS_AFR672C; -.
DR   eggNOG; KOG1974; Eukaryota.
DR   HOGENOM; CLU_008440_0_0_1; -.
DR   InParanoid; Q752A3; -.
DR   OMA; WLKLYDE; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0031298; C:replication fork protection complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR   GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0043570; P:maintenance of DNA repeat elements; IEA:EnsemblFungi.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0007064; P:mitotic sister chromatid cohesion; IEA:EnsemblFungi.
DR   GO; GO:0043111; P:replication fork arrest; IBA:GO_Central.
DR   GO; GO:0048478; P:replication fork protection; IBA:GO_Central.
DR   InterPro; IPR044998; Timeless.
DR   InterPro; IPR006906; Timeless_N.
DR   PANTHER; PTHR22940; PTHR22940; 1.
DR   Pfam; PF04821; TIMELESS; 1.
PE   3: Inferred from homology;
KW   Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..1265
FT                   /note="Topoisomerase 1-associated factor 1"
FT                   /id="PRO_0000301727"
FT   REGION          650..670
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1019..1052
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1167..1226
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1265 AA;  144220 MW;  29939D41962BE089 CRC64;
     MEEMVSQNTE GDKSIDDHWQ TEGAETQPGL HETFPLGVLK ARVALLATAI GGPDHTSEQE
     AAPYKLGDDC LACLKDLKRW FVLVDQKQKR WDVASAAAEF RILEEDLIPI LVQWEQKMSQ
     GIKQSKVSGL PLSECIKNKQ YHGKIALNAF QLMVWMTWPM TLNEESSKNQ VHHYSGLKKH
     QLAYKKAILQ AHGGKVVKAA VRIATEVIKI DRLDRSVQDN SLIRLVLNFL RNILAIEPGE
     VTVSAKQLSK SRNINTSDML PPNISVDDIC LNSVVSVFKK FKVFPFLLTI SSSMGHEFEA
     EYVCLPLLEV AFYLTKDIDP HLLSLKADNP PQSLQNCGNK VSNGQGVVSG QLGMELIHLL
     DKERHHKMSQ IRSSSTRHSR FGGFFSIQTP NNGRLTISNG HSALDHDLAL NNIDKKKKWN
     KTTRNTAEAV QGIPVGVLNG EFNLTFLSHD NRSCLKWFLA NFVDSSFNIL LKNLIDYFTA
     DEFNQQILQK LQFLLFYAWF LKFERDRCQK GETDGIFVSE ALQDTSHILI IKFLREAHEL
     KKWPLVHAAM LAITELLNYL DVLGEDWEED VVGIYTKIFS NERLKLFSTI PRTASRHSLH
     YVKACINMNH AIMTTLERFN KNNKDLIIKG FRKRMITRFI VKEGKTTSGD VNGNKNGQDT
     TRDADEDAIS SDEETNMISA RLDFKKVLDA YFNGTVIDTY IFYLKHFTEL TEEDIKRVLY
     FLNHILCSNE ESFLFRVDFM IVLKEMLAPG GLSVTGHTRI RINEFSDHFM AKLKRKLEKS
     PFWYINILFP NLHDRELGYY LRYGQQRPNT QSIHKVVAPT MFKHIEGEEN MSEQQLKDLK
     IGVLVSTLLD DGKNHFVEEI NRIFKLVVEG YEKHYQNNER SATLHYPRVD FRSDMFDMKR
     ALLFDSDLRA LMTTLGYHVA DTEVEPCYIG GIFYFPDLSS ALNSLEQYMA IPFHTPNGLP
     SSSYLLRSID YKSGVDADAK VGEAMRIDQF SDEMADMALS ENANRYFEDL NRMEERLEGK
     QIPRGTAKKR SAIKPKSRKS QPTGIGGIDD DTPVINKSKR KKQLFLSNEF IMDSDDEEEL
     VASPIFYENE IYLRFILNKY QGNLPSHLFS VFSRFANERG RMAGAVVGDY TDLFNGPVPS
     IEELKSMENS TTHHGLKEIL QLQPRDHLSL SPNNENNSAH SSENLSSDSE NDNITNDIPL
     SDSEYNSSNS SQYANTAKTS IIDPPSSKTS LLKLALTHEA DVESEDDFIP RKRARKVRLE
     DGEEY