TOF1_ASPFU
ID TOF1_ASPFU Reviewed; 1176 AA.
AC Q4W9M7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Topoisomerase 1-associated factor 1;
GN Name=tof1; ORFNames=AFUA_4G04390;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
CC -!- FUNCTION: Forms a fork protection complex (FPC) with csm3 and which is
CC required for chromosome segregation during meiosis and DNA damage
CC repair. FPC coordinates leading and lagging strand synthesis and moves
CC with the replication fork. FPC stabilizes replication forks in a
CC configuration that is recognized by replication checkpoint sensors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC tof1 and csm3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR EMBL; AAHF01000016; EAL84586.2; -; Genomic_DNA.
DR RefSeq; XP_746624.2; XM_741531.2.
DR AlphaFoldDB; Q4W9M7; -.
DR SMR; Q4W9M7; -.
DR STRING; 746128.CADAFUBP00009583; -.
DR PRIDE; Q4W9M7; -.
DR EnsemblFungi; EAL84586; EAL84586; AFUA_4G04390.
DR GeneID; 3503983; -.
DR KEGG; afm:AFUA_4G04390; -.
DR VEuPathDB; FungiDB:Afu4g04390; -.
DR eggNOG; KOG1974; Eukaryota.
DR HOGENOM; CLU_004390_0_0_1; -.
DR InParanoid; Q4W9M7; -.
DR OMA; WLKLYDE; -.
DR OrthoDB; 839367at2759; -.
DR Proteomes; UP000002530; Chromosome 4.
DR GO; GO:0031298; C:replication fork protection complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0043111; P:replication fork arrest; IBA:GO_Central.
DR GO; GO:0048478; P:replication fork protection; IBA:GO_Central.
DR InterPro; IPR044998; Timeless.
DR InterPro; IPR007725; TIMELESS_C.
DR InterPro; IPR006906; Timeless_N.
DR PANTHER; PTHR22940; PTHR22940; 1.
DR Pfam; PF04821; TIMELESS; 1.
DR Pfam; PF05029; TIMELESS_C; 1.
PE 3: Inferred from homology;
KW Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..1176
FT /note="Topoisomerase 1-associated factor 1"
FT /id="PRO_0000301729"
FT REGION 569..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 791..815
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 879..965
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 977..1000
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1021..1159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 885..909
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1021..1042
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1043..1058
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1116..1135
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1138..1152
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1176 AA; 134897 MW; 236EADF236015816 CRC64;
MDEEGAPLSE GVQVVDPDVR AHVYSLVTAL GGFNGESADR YVLGDDALAC LRDIKRWLKL
YDEKHNRMDV ARCLGEANLV NGDLLPILTL WSTSGQKSKH MSRIALACLE LLVPLTWPLE
VHSEMTVNHH RHTPYLQQAQ VLYKRGILSH GSGSILRTII RIGLPSMAVP RSERTTRDEG
ILKLMLYFLR NIAVISPNAR LAAEGDEEET SRSATINAFQ NQDAFALLLT MCSNVGEDFS
LQDVVLLEIL FHIVKGVNVE KLFMNDAQRK AKRTDELGEL LQKESSLRRE YAKNAPTRHG
RFGTMIWVKR DDAKVSTVSG QDVLKDSQTT LHKMDQSKKW NKPQIRRRAA EVTANNDFNT
PVNINSTATK NLRMFVEEFL DSGFNPLFTH VRKAIEREAD RVMDINTRQF FYTVAWFLEA
ERVRRAHQRE KRHLGDTPLK EIEPDSFALV ASVLNQETFV FLNRSMQYSY DNKDVEDLTA
EMRCFTQILL TVQEMAQSPL EEDQEIADNI QNRIFYEETT HDRIVAIVRG YKDQGFGYLD
ACTELAHVFL RMLEHYSKEN VDMHVRSRRR AKRKAKQAKQ ADIEGDDEEE ASEEEDLMDV
ERISKERKFD FRRFAAKFCN QSCVDTFIAF TKYYRELNVD QLKRAHRYFY RIAFKQEMSV
LLFRLDIINL FYRMIKGPGA LDSNKPIFKE WEELVRQIIR RMIKKIDQRP ALITELLFSK
INSTVFYLEY GHEKQTISAS KRPPAELEVE PREAKTIDEK IRIVVHVMVK DEHTDLVKWV
SDVLNSAADE REAWESQEQH SGGQKAPNPM IPVKSDNESC QKAMFSNAKL RLLMSLVRFE
RLGMEDVPGA SWVVPSSLNS QELRHTRSII EQCLTEPVTE NSDRDLSQLI RRKSGNNTRR
DRDDQMANVD FGSDSEGDDN VPDGPLFPPN PRSRANALEQ LKKQRKKRRK QAGEEEEPDE
EDLEARRRAR LENALARQAK IKSDLYIHAS DEETDEEADQ EFFRLEEQRR KEQAERIRKA
LLHGVVEEVS ENSRKKSSGR KRQSDQYTAS TADSQSKRQR RLQQTEGLDG NDDLVVAGTE
ARSPDSLGQG SPSLKGANDV EDTLVTSEEN ELDFDDDLAF SRNRTRDKVL SAENDDSDTE
PPAPDTIDED GEEAAAVAAP PRRRVRAGFV IESDSE
